Overview

  • Product name

    Anti-PCPTP1/PTPRQ antibody
    See all PCPTP1/PTPRQ primary antibodies
  • Description

    Rabbit polyclonal to PCPTP1/PTPRQ
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Dog, Chimpanzee
  • Immunogen

    Synthetic peptide corresponding to Human PCPTP1/PTPRQ aa 400-500 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab24482)

  • General notes

     This product was previously labelled as PCPTP1

     

Properties

Applications

Our Abpromise guarantee covers the use of ab12153 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/40. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 0.4 µg/ml. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa).

Target

  • Function

    Sequesters mitogen-activated protein kinases (MAPKs) such as MAPK1, MAPK3 and MAPK14 in the cytoplasm in an inactive form. The MAPKs bind to a dephosphorylated kinase interacting motif, phosphorylation of which by the protein kinase A complex releases the MAPKs for activation and translocation into the nucleus.
  • Tissue specificity

    Expressed in brain, placenta, small intestine, stomach, uterus and weakly in the prostate. Isoform alpha has been observed only in the brain. Isoform gamma is expressed in brain, placenta and uterus. Isoform delta is expressed in brain, kidney, placenta, prostate, small intestine and uterus.
  • Sequence similarities

    Belongs to the protein-tyrosine phosphatase family. Receptor class 7 subfamily.
    Contains 1 tyrosine-protein phosphatase domain.
  • Cellular localization

    Cell membrane and Cytoplasm > perinuclear region. Locates to the perinuclear areas within the cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Ch-1PTPase antibody
    • Ch1 PTPase antibody
    • DKFZp781C1038 antibody
    • EC PTP antibody
    • ECPTP antibody
    • FLJ34328 antibody
    • MGC131968 antibody
    • MGC148170 antibody
    • NC PTPCOM1 antibody
    • NC-PTPCOM1 antibody
    • Protein tyrosine phosphatase Cr1PTPase precursor antibody
    • Protein tyrosine phosphatase NC PTPCOM1 antibody
    • Protein tyrosine phosphatase PCPTP1 antibody
    • Protein tyrosine phosphatase receptor type R antibody
    • Protein-tyrosine phosphatase PCPTP1 antibody
    • PTP SL antibody
    • PTPBR7 antibody
    • PTPRQ antibody
    • PTPRR antibody
    • PTPRR_HUMAN antibody
    • PTPSL antibody
    • R-PTP-R antibody
    • Receptor type tyrosine protein phosphatase R antibody
    • Receptor-type tyrosine-protein phosphatase R antibody
    see all

Images

  • All lanes : Anti-PCPTP1/PTPRQ antibody (ab12153) at 0.4 µg/ml

    Lane 1 : 0.01µg PTP beta (PTP RB)
    Lane 2 : 0.01µg PTP epsilon (PTP RE)
    Lane 3 : 0.01µg LAR (PTP RF)
    Lane 4 : 0.01µg PTP gamma (PTP RG)
    Lane 5 : 0.01µg SAP1 (PTP RH)
    Lane 6 : 0.01µg DEP1 (PTP RJ)
    Lane 7 : 0.01µg PTP kappa (PTP RK)
    Lane 8 : 0.01µg PTPIA2 (PTP RN)
    Lane 9 : 0.01µg GLEPP1 (PTP RO)
    Lane 10 : 0.01µg PCPTP1 (PTP RR)
    Lane 11 : 0.01µg CD45 (PTP RC)

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 0.2 µg/ml

    Performed under reducing conditions.

    Predicted band size: 73 kDa
    Observed band size: 73 kDa



    Recombinant PTP phosphatase domains (0.01µg per lane) were probed with ab12153 at 1/2500 followed by secondary goat anti-rabbit IgG antibody (ab6721 at 1/5000):

    Exposure time: 30s

    Lane 1: PTP beta  (PTP RB)
    Lane 2: PTP epsilon  (PTP RE)
    Lane 3: LAR  (PTP RF)
    Lane 4: PTP gamma (PTP RG)
    Lane 5: SAP1  (PTP RH)
    Lane 6: DEP1  (PTP RJ)
    Lane 7: PTP kappa   (PTP RK)
    Lane 8: PTPIA2  (PTP RN)
    Lane 9: GLEPP1  (PTP RO)
    Lane 10: PCPTP1/PTPRQ  (PTP RR)
    Lane 11: CD45  (PTP RC)

    Recombinant PCPTP1/PTPRQ phosphatase domains (0.01µg per lane) were probed with ab12153 at 0.4µg/ml

  • Image courtesy of Human Protein Atlas    

    ab12153 staining PCPTP1/PTPRQ in Human cerebellum. The paraffin embedded tissue was incubated with ab12153 (1/40 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. ab12153 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines.

    Further results for this antibody can be found at

References

This product has been referenced in:

  • Su PH  et al. Epigenetic silencing of PTPRR activates MAPK signaling, promotes metastasis and serves as a biomarker of invasive cervical cancer. Oncogene 32:15-26 (2013). Read more (PubMed: 22330137) »
See 1 Publication for this product

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