Recombinant Anti-PTPRR/PCPTP1 antibody [EPR13188] - BSA and Azide free (ab250146)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13188] to PTPRR/PCPTP1 - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
-
Product name
Anti-PTPRR/PCPTP1 antibody [EPR13188] - BSA and Azide free
See all PTPRR/PCPTP1 primary antibodies -
Description
Rabbit monoclonal [EPR13188] to PTPRR/PCPTP1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
General notes
ab250146 is the carrier-free version of ab180134.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13188 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Conjugation kits
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab250146 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use at an assay dependent concentration. Predicted molecular weight: 74 kDa.
|
Notes |
---|
WB
Use at an assay dependent concentration. Predicted molecular weight: 74 kDa. |
Target
-
Function
Sequesters mitogen-activated protein kinases (MAPKs) such as MAPK1, MAPK3 and MAPK14 in the cytoplasm in an inactive form. The MAPKs bind to a dephosphorylated kinase interacting motif, phosphorylation of which by the protein kinase A complex releases the MAPKs for activation and translocation into the nucleus. -
Tissue specificity
Expressed in brain, placenta, small intestine, stomach, uterus and weakly in the prostate. Isoform alpha has been observed only in the brain. Isoform gamma is expressed in brain, placenta and uterus. Isoform delta is expressed in brain, kidney, placenta, prostate, small intestine and uterus. -
Sequence similarities
Belongs to the protein-tyrosine phosphatase family. Receptor class 7 subfamily.
Contains 1 tyrosine-protein phosphatase domain. -
Cellular localization
Cell membrane and Cytoplasm > perinuclear region. Locates to the perinuclear areas within the cytoplasm. - Information by UniProt
-
Database links
- Entrez Gene: 5801 Human
- Entrez Gene: 19279 Mouse
- Entrez Gene: 94202 Rat
- Omim: 602853 Human
- SwissProt: Q15256 Human
- SwissProt: Q62132 Mouse
- SwissProt: O08617 Rat
- Unigene: 506076 Human
see all -
Alternative names
- Ch-1PTPase antibody
- Ch1 PTPase antibody
- DKFZp781C1038 antibody
see all
Images
-
All lanes : Anti-PTPRR/PCPTP1 antibody [EPR13188] (ab180134) at 1/5000 dilution
Lane 1 : Human cerebellum lysate at 20 µg
Lane 2 : Mouse brain lysate at 20 µg
Lane 3 : Rat brain lysate at 10 µg
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 74 kDaThis data was developed using ab180134, the same antibody clone in a different buffer formulation.
-
All lanes : Anti-PTPRR/PCPTP1 antibody [EPR13188] (ab180134) at 1/1000 dilution
Lane 1 : HEK293 (Human embryonic kidney epithelial cell) whole cell lysate
Lane 2 : SK-OV-3 (Human ovarian cancer epithelial cell
) whole cell lysate
Lane 3 : HCT 116 (Human colorectal carcinoma epithelial cell) whole cell lysate
Lane 4 : Human cerebellum tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/20000 dilution
Predicted band size: 74 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Exposure time: 20 secondsBlocking buffer and concentration : 5% NFDM/TBST
Diluting buffer and concentration : 5% NFDM /TBSTThis data was developed using ab180134, the same antibody clone in a different buffer formulation.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
Certificate of Compliance
References (0)
ab250146 has not yet been referenced specifically in any publications.