RecombinantRabMAb

Recombinant Anti-PCQAP / MED15 antibody [EPR13309] - BSA and Azide free (ab250386)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCQAP / MED15 antibody [EPR13309] - BSA and Azide free (ab250386)
  • Flow Cytometry (Intracellular) - Anti-PCQAP / MED15 antibody [EPR13309] - BSA and Azide free (ab250386)
  • Western blot - Anti-PCQAP / MED15 antibody [EPR13309] - BSA and Azide free (ab250386)
  • Western blot - Anti-PCQAP / MED15 antibody [EPR13309] - BSA and Azide free (ab250386)
  • Immunocytochemistry - Anti-PCQAP / MED15 antibody [EPR13309] - BSA and Azide free (ab250386)
  • Flow Cytometry (Intracellular) - Anti-PCQAP / MED15 antibody [EPR13309] - BSA and Azide free (ab250386)
  • Immunocytochemistry - Anti-PCQAP / MED15 antibody [EPR13309] - BSA and Azide free (ab250386)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCQAP / MED15 antibody [EPR13309] - BSA and Azide free (ab250386)
  • Western blot - Anti-PCQAP / MED15 antibody [EPR13309] - BSA and Azide free (ab250386)
  • Anti-PCQAP / MED15 antibody [EPR13309] - BSA and Azide free (ab250386)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13309] to PCQAP / MED15 - BSA and Azide free
  • Suitable for: ICC, Flow Cyt (Intra), WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

Overview

  • Product name

    Anti-PCQAP / MED15 antibody [EPR13309] - BSA and Azide free
    See all PCQAP / MED15 primary antibodies

  • Description

    Rabbit monoclonal [EPR13309] to PCQAP / MED15 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, Flow Cyt (Intra), WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab250386 is the carrier-free version of ab181158.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR13309

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab250386 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC
Use at an assay dependent concentration.
Flow Cyt (Intra)
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 97 kDa (predicted molecular weight: 87 kDa).
IHC-P
Use at an assay dependent concentration.

The mouse and rat recommendation is based on the WB results. This antibody may not be suitable for IHC with mouse or rat samples.

 
Notes
ICC
Use at an assay dependent concentration.
Flow Cyt (Intra)
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 97 kDa (predicted molecular weight: 87 kDa).
IHC-P
Use at an assay dependent concentration.

The mouse and rat recommendation is based on the WB results. This antibody may not be suitable for IHC with mouse or rat samples.

 

Target

  • Relevance

    PCQAP / MED15 is a member of the mediator complex that can act as both a positive and negative regulator of transcription. The mediator complex interacts with RNA pol II and its associated proteins, the general transcription factors. Mediator proteins serve as a bridge connecting the activator and basal transcription machinery.

  • Cellular localization

    Cytoplasmic and Nuclear

  • Database links

    • Alternative names

      • Activator recruited cofactor 105 kD antibody
      • Activator-recruited cofactor 105 kDa component antibody
      • ARC105 antibody
      • CAG7A antibody
      • CTG repeat protein 7a antibody
      • CTG7A antibody
      • Mediator complex subunit 15 antibody
      • Mediator of RNA polymerase II transcription subunit 15 antibody
      • PC2 glutamine rich associated protein antibody
      • PC2 glutamine/Q-rich-associated protein antibody
      • Positive cofactor 2 glutamine/Q-rich-associated protein antibody
      • TIG-1 antibody
      • TIG1 antibody
      • TNRC7 antibody
      • TPA inducible gene 1 antibody
      • TPA inducible protein antibody
      • TPA-inducible gene 1 protein antibody
      • Trinucleotide repeat containing 7 antibody
      • Trinucleotide repeat containing gene 7 protein antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCQAP / MED15 antibody [EPR13309] - BSA and Azide free (ab250386)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCQAP / MED15 antibody [EPR13309] - BSA and Azide free (ab250386)

      This data was developed using ab181158, the same antibody clone in a different buffer formulation.

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung carcinoma tissue sections labeling PCQAP/MED15 with purified ab181158 at 1:250 dilution (1.28 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
    • Flow Cytometry (Intracellular) - Anti-PCQAP / MED15 antibody [EPR13309] - BSA and Azide free (ab250386)
      Flow Cytometry (Intracellular) - Anti-PCQAP / MED15 antibody [EPR13309] - BSA and Azide free (ab250386)

      This data was developed using ab181158, the same antibody clone in a different buffer formulation. 

      Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling PCQAP/MED15 with purified ab181158 at 1/30 dilution (10 ug/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1/2000 dilution. Isotype control - 90% methanol. Unlabeled control - Rabbit monoclonal IgG (Black). Cell without incubation with primary antibody and secondary antibody (Blue). 

       

       

    • Western blot - Anti-PCQAP / MED15 antibody [EPR13309] - BSA and Azide free (ab250386)
      Western blot - Anti-PCQAP / MED15 antibody [EPR13309] - BSA and Azide free (ab250386)
      All lanes : Anti-PCQAP / MED15 antibody [EPR13309] (ab181158) at 1/1000 dilution (purified)

      Lane 1 : 293 (Human embryonic kidney epithelial cell) whole cell lysates
      Lane 2 : K562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates
      Lane 3 : C6 (Rat glial tumor glial cell) whole cell lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 87 kDa
      Observed band size: 97 kDa why is the actual band size different from the predicted?



      This data was developed using ab181158, the same antibody clone in a different buffer formulation.

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-PCQAP / MED15 antibody [EPR13309] - BSA and Azide free (ab250386)
      Western blot - Anti-PCQAP / MED15 antibody [EPR13309] - BSA and Azide free (ab250386)
      All lanes : Anti-PCQAP / MED15 antibody [EPR13309] (ab181158) at 1/1000 dilution (purified)

      Lane 1 : Mouse placenta lysates
      Lane 2 : Mouse spleen lysates

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 87 kDa



      This data was developed using ab181158, the same antibody clone in a different buffer formulation.

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Immunocytochemistry - Anti-PCQAP / MED15 antibody [EPR13309] - BSA and Azide free (ab250386)
      Immunocytochemistry - Anti-PCQAP / MED15 antibody [EPR13309] - BSA and Azide free (ab250386)
      This data was developed using ab181158, the same antibody clone in a different buffer formulation.Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling PCQAP/MED15 with Purified ab181158 at 1:100 dilution (3.2μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    • Flow Cytometry (Intracellular) - Anti-PCQAP / MED15 antibody [EPR13309] - BSA and Azide free (ab250386)
      Flow Cytometry (Intracellular) - Anti-PCQAP / MED15 antibody [EPR13309] - BSA and Azide free (ab250386)

      This data was developed using ab181158, the same antibody clone in a different buffer formulation. 

      Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed 293 cells labeling PCQAP / MED15 with unpurified ab181158 at 1/50 dilution (red), compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution. 

       

       

    • Immunocytochemistry - Anti-PCQAP / MED15 antibody [EPR13309] - BSA and Azide free (ab250386)
      Immunocytochemistry - Anti-PCQAP / MED15 antibody [EPR13309] - BSA and Azide free (ab250386)
      This data was developed using ab181158, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of acetone-fixed HeLa cells labeling PCQAP / MED15 with unpurified ab181158 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 diution. Counter stained with Dapi (blue).
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCQAP / MED15 antibody [EPR13309] - BSA and Azide free (ab250386)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCQAP / MED15 antibody [EPR13309] - BSA and Azide free (ab250386)
      This data was developed using ab181158, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling PCQAP / MED15 with unpurified ab181158 at 1/250 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counterstained with Hematoxylin.
    • Western blot - Anti-PCQAP / MED15 antibody [EPR13309] - BSA and Azide free (ab250386)
      Western blot - Anti-PCQAP / MED15 antibody [EPR13309] - BSA and Azide free (ab250386)
      All lanes : Anti-PCQAP / MED15 antibody [EPR13309] (ab181158) at 1/1000 dilution (unpurified)

      Lane 1 : 293 cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : HeLa cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

      Predicted band size: 87 kDa
      Observed band size: 97 kDa why is the actual band size different from the predicted?



      This data was developed using ab181158, the same antibody clone in a different buffer formulation.

       

    • Anti-PCQAP / MED15 antibody [EPR13309] - BSA and Azide free (ab250386)
      Anti-PCQAP / MED15 antibody [EPR13309] - BSA and Azide free (ab250386)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab250386? Please let us know so that we can cite the reference in this datasheet.

    ab250386 has not yet been referenced specifically in any publications.

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