Anti-PCQAP / MED15 antibody (ab176593)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-PCQAP / MED15 antibody
See all PCQAP / MED15 primary antibodies -
Description
Rabbit polyclonal to PCQAP / MED15 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig
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Immunogen
Synthetic peptide within Human PCQAP/ MED15 aa 450-500. The exact sequence is proprietary.
Sequence:P PPQPSPQPGQ PSSQPNSNVS SGPAPSPSSF LPSPSPQPSQ SPVTARTPQN
Database link: Q96RN5 -
Positive control
- Human Breast Carcinoma, Prostate Carcinoma and Testicular Seminoma tissues.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab176593 was affinity purified using an epitope specific to PCQAP / MED15 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab176593 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/50 - 1/500. | |
| IHC-P | 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Relevance
PCQAP / MED15 is a member of the mediator complex that can act as both a positive and negative regulator of transcription. The mediator complex interacts with RNA pol II and its associated proteins, the general transcription factors. Mediator proteins serve as a bridge connecting the activator and basal transcription machinery. -
Cellular localization
Cytoplasmic and Nuclear -
Database links
- Entrez Gene: 51586 Human
- Entrez Gene: 94112 Mouse
- Entrez Gene: 360743 Rat
- Omim: 607372 Human
- SwissProt: Q96RN5 Human
- SwissProt: Q924H2 Mouse
- Unigene: 517421 Human
- Unigene: 208970 Mouse
see all -
Alternative names
- Activator recruited cofactor 105 kD antibody
- Activator-recruited cofactor 105 kDa component antibody
- ARC105 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCQAP / MED15 antibody (ab176593)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast carcinoma tissue labeling PCQAP / MED15 with ab176593 at 1/250 dilution. Detection: DAB staining.
Datasheets and documents
References
ab176593 has not yet been referenced specifically in any publications.
