Anti-PCQAP / MED15 antibody (ab176593)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCQAP / MED15 antibody (ab176593)

    Key features and details

    • Rabbit polyclonal to PCQAP / MED15
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG

    Overview

    • Product name

      Anti-PCQAP / MED15 antibody
      See all PCQAP / MED15 primary antibodies

    • Description

      Rabbit polyclonal to PCQAP / MED15

    • Host species

      Rabbit

    • Tested applications

      Suitable for: IHC-Pmore details

    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig

    • Immunogen

      Synthetic peptide within Human PCQAP/ MED15 aa 450-500. The exact sequence is proprietary.
      Sequence:

      P PPQPSPQPGQ PSSQPNSNVS SGPAPSPSSF LPSPSPQPSQ SPVTARTPQN


      Database link: Q96RN5
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • Positive control

      • Human Breast Carcinoma, Prostate Carcinoma and Testicular Seminoma tissues.

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

    • Storage buffer

      pH: 6.8
      Preservative: 0.09% Sodium azide
      Constituents: 99% Tris buffered saline, 0.1% BSA
    • Concentration information loading...

    • Purity

      Immunogen affinity purified

    • Purification notes

      ab176593 was affinity purified using an epitope specific to PCQAP / MED15 immobilized on solid support.

    • Clonality

      Polyclonal

    • Isotype

      IgG

    • Research areas

    Applications

    Our Abpromise guarantee covers the use of ab176593 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    Target

    • Relevance

      PCQAP / MED15 is a member of the mediator complex that can act as both a positive and negative regulator of transcription. The mediator complex interacts with RNA pol II and its associated proteins, the general transcription factors. Mediator proteins serve as a bridge connecting the activator and basal transcription machinery.

    • Cellular localization

      Cytoplasmic and Nuclear

    • Database links

      see all

    • Alternative names

      • Activator recruited cofactor 105 kD antibody
      • Activator-recruited cofactor 105 kDa component antibody
      • ARC105 antibody
      • CAG7A antibody
      • CTG repeat protein 7a antibody
      • CTG7A antibody
      • Mediator complex subunit 15 antibody
      • Mediator of RNA polymerase II transcription subunit 15 antibody
      • PC2 glutamine rich associated protein antibody
      • PC2 glutamine/Q-rich-associated protein antibody
      • Positive cofactor 2 glutamine/Q-rich-associated protein antibody
      • TIG-1 antibody
      • TIG1 antibody
      • TNRC7 antibody
      • TPA inducible gene 1 antibody
      • TPA inducible protein antibody
      • TPA-inducible gene 1 protein antibody
      • Trinucleotide repeat containing 7 antibody
      • Trinucleotide repeat containing gene 7 protein antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCQAP / MED15 antibody (ab176593)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCQAP / MED15 antibody (ab176593)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast carcinoma tissue labeling PCQAP / MED15 with ab176593 at 1/250 dilution. Detection: DAB staining.

    References (0)

    Publishing research using ab176593? Please let us know so that we can cite the reference in this datasheet.

    ab176593 has not yet been referenced specifically in any publications.

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