Anti-PCQAP / MED15 antibody (ab176593)
Key features and details
- Rabbit polyclonal to PCQAP / MED15
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PCQAP / MED15 antibody
See all PCQAP / MED15 primary antibodies -
Description
Rabbit polyclonal to PCQAP / MED15 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig -
Immunogen
Synthetic peptide within Human PCQAP/ MED15 aa 450-500. The exact sequence is proprietary.
Sequence:P PPQPSPQPGQ PSSQPNSNVS SGPAPSPSSF LPSPSPQPSQ SPVTARTPQN
Database link: Q96RN5 -
Positive control
- Human Breast Carcinoma, Prostate Carcinoma and Testicular Seminoma tissues.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab176593 was affinity purified using an epitope specific to PCQAP / MED15 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab176593 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P | 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Relevance
PCQAP / MED15 is a member of the mediator complex that can act as both a positive and negative regulator of transcription. The mediator complex interacts with RNA pol II and its associated proteins, the general transcription factors. Mediator proteins serve as a bridge connecting the activator and basal transcription machinery. -
Cellular localization
Cytoplasmic and Nuclear -
Database links
- Entrez Gene: 51586 Human
- Entrez Gene: 94112 Mouse
- Entrez Gene: 360743 Rat
- Omim: 607372 Human
- SwissProt: Q96RN5 Human
- SwissProt: Q924H2 Mouse
- Unigene: 517421 Human
- Unigene: 208970 Mouse
see all -
Alternative names
- Activator recruited cofactor 105 kD antibody
- Activator-recruited cofactor 105 kDa component antibody
- ARC105 antibody
see all
Images
Datasheets and documents
References (0)
ab176593 has not yet been referenced specifically in any publications.