Overview

  • Product name
  • Description
    Rabbit polyclonal to PCSK2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Pig
  • Immunogen

    Synthetic peptide corresponding to Mouse PCSK2 aa 622-638.
    Sequence:

    ELDEAVERSLQSILRKN


    (Peptide available as ab5012)

  • Positive control
    • WB: TC 6 cell extract.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Primary antibody notes
    The subtilisin-like Prohormone Convertase (PC) family is a group of cellular enzymes that cleave most prohormones and neuropeptide precursors. Numerous other cellular proteins, some viral proteins, and bacterial toxins that are transported by the constitutive secretory pathway are also targeted for maturation by PCs. PC family members share structural similarities, which include a heterogeneous ~10 kDa amino-terminal proregion, a highly conserved ~55 kDa subtilisin-like catalytic domain, and carboxyl-terminal domain that is heterogeneous in length and sequence. These enzymes become catalytically active following proregion cleavage within the appropriate cellular compartment. The subcellular localization of PC family members varies. Immunolocalization studies show that PC1 is found in the perinuclear region as well as the trans-Golgi network, whereas PC2 can be found in the trans-Golgi network as well as diffusely distributed in the peripheral cytoplasm.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3533 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/20 - 1/200.
ICC/IF 1/20 - 1/200.
WB Use a concentration of 2 µg/ml. Can be blocked with PCSK2 peptide (ab5012).

This antibody detects an ~65 kDa protein representing Proprotein 2 from alpha TC 6 cell extract.

Target

Images

  • Anti-PCSK2 antibody (ab3533) at 1/500 dilution + Mouse cerebellum cell lysate at 25 µg

    Observed band size: 65 kDa
    why is the actual band size different from the predicted?

  • Immunocytochemistry/Immunofluorescent analysis of PCSK2 (green) showing staining in the cytoplasm of HEK293 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab3533 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 �C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

  • Immunocytochemistry/Immunofluorescent analysis of PCSK2 (green) showing staining in the cytoplasm of C2C12 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab3533 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 �C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

References

This product has been referenced in:
  • Du W  et al. HID-1 is required for homotypic fusion of immature secretory granules during maturation. Elife 5:N/A (2016). WB, IF ; Mouse . Read more (PubMed: 27751232) »
  • Gagnon J  et al. Expression of PCSK1 (PC1/3), PCSK2 (PC2) and PCSK3 (furin) in mouse small intestine. Regul Pept 152:54-60 (2009). Read more (PubMed: 18706454) »
See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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