Product nameAnti-PCSK9 antibody
See all PCSK9 primary antibodies
DescriptionGoat polyclonal to PCSK9
Tested applicationsSuitable for: WBmore details
Predicted to work with: Human, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
- WB: HEK-293 cell lysate overexpressing recombinant human PCSK9.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
Our Abpromise guarantee covers the use of ab223669 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 80-100 kDa (predicted molecular weight: 74 kDa).|
FunctionMay be implicated in the differentiation of cortical neurons and may play a role in cholesterol homeostasis.
Tissue specificityExpressed in neuro-epithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells.
Involvement in diseaseDefects in PCSK9 are the cause of familial hypercholesterolemia 3 (FH3) [MIM:603776]. FH3 inheritance is autosomal dominant.
Sequence similaritiesBelongs to the peptidase S8 family.
Contains 1 peptidase S8 domain.
modificationsThe soluble zymogen undergoes autocatalytic intramolecular processing in the endoplasmic reticulum, resulting in the cleavage of its propeptide that remains associated with the secreted enzyme.
- Information by UniProt
- Convertase subtilisin/kexin type 9 preproprotein antibody
- FH3 antibody
- HCHOLA3 antibody
Lane 1 : Anti-PCSK9 antibody (ab223669) at 0.5 µg/ml
Lane 2 : Anti-PCSK9 antibody (ab223669) at 1 µg/ml
Lane 3 : Anti-Myc tag at 1/1000 dilution
Lanes 1 & 3 : HEK-293 (human epithelial cell line from embryonic kidney) cells overexpressing recombinant human PCSK9 with C-terminal Myc tag, whole cell lysate
Lane 2 : Mock transfected HEK-293 cell lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Predicted band size: 74 kDa
Observed band size: 80-100 kDa why is the actual band size different from the predicted?
Primary incubations were for 1 hour
ab223669 has not yet been referenced specifically in any publications.