Overview

  • Product name

  • Description

    Rabbit polyclonal to PCSK9
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Monkey, Gorilla
  • Immunogen

    Synthetic peptide within Human PCSK9 aa 450-500. The exact sequence is proprietary.
    Database link: Q8NBP7

  • Positive control

    • IP: HEK-293T whole cell lysate.
  • General notes

    ab245361 has not performed satisfactorily when used for WB of PCSK9 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of PCSK9.

Properties

Applications

Our Abpromise guarantee covers the use of ab245361 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function

      May be implicated in the differentiation of cortical neurons and may play a role in cholesterol homeostasis.
    • Tissue specificity

      Expressed in neuro-epithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells.
    • Involvement in disease

      Defects in PCSK9 are the cause of familial hypercholesterolemia 3 (FH3) [MIM:603776]. FH3 inheritance is autosomal dominant.
    • Sequence similarities

      Belongs to the peptidase S8 family.
      Contains 1 peptidase S8 domain.
    • Post-translational
      modifications

      The soluble zymogen undergoes autocatalytic intramolecular processing in the endoplasmic reticulum, resulting in the cleavage of its propeptide that remains associated with the secreted enzyme.
    • Cellular localization

      Secreted.
    • Information by UniProt
    • Database links

    • Alternative names

      • Convertase subtilisin/kexin type 9 preproprotein antibody
      • FH3 antibody
      • HCHOLA3 antibody
      • Hypercholesterolemia autosomal dominant 3 antibody
      • LDLCQ1 antibody
      • NARC 1 antibody
      • NARC-1 antibody
      • NARC1 antibody
      • Neural apoptosis regulated convertase 1 antibody
      • Neural apoptosis-regulated convertase 1 antibody
      • PC 9 antibody
      • PC9 antibody
      • PCSK 9 antibody
      • PCSK9 antibody
      • PCSK9_HUMAN antibody
      • Proprotein convertase 9 antibody
      • Proprotein convertase PC9 antibody
      • Proprotein convertase subtilisin/kexin type 9 antibody
      • PSEC0052 antibody
      • Subtilisin/kexin like protease PC9 antibody
      • Subtilisin/kexin-like protease PC9 antibody
      see all

    Images

    • PCSK9 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245361 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab245361 at 1 µg/ml.

      Lane 1: ab245361 IP in HEK-293T whole cell lysate.
      Lane 2: Control IgG IP in HEK-293T whole cell lysate.

      Detection: Chemiluminescence with exposure time of 3 minutes.

      Lysates prepared in NETN buffer.

    References

    ab245361 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab245361.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up