Overview

  • Product name
  • Description
    Goat polyclonal to PCSK9
  • Host species
    Goat
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    CRSRHLAQASQELQ

    , corresponding to C terminal amino acids 679-692 of Human PCSK9

  • Positive control
    • Human liver, pancreas and adipose lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab28770 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 74 kDa.
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 2 - 6 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function
    May be implicated in the differentiation of cortical neurons and may play a role in cholesterol homeostasis.
  • Tissue specificity
    Expressed in neuro-epithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells.
  • Involvement in disease
    Defects in PCSK9 are the cause of familial hypercholesterolemia 3 (FH3) [MIM:603776]. FH3 inheritance is autosomal dominant.
  • Sequence similarities
    Belongs to the peptidase S8 family.
    Contains 1 peptidase S8 domain.
  • Post-translational
    modifications
    The soluble zymogen undergoes autocatalytic intramolecular processing in the endoplasmic reticulum, resulting in the cleavage of its propeptide that remains associated with the secreted enzyme.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Convertase subtilisin/kexin type 9 preproprotein antibody
    • FH3 antibody
    • HCHOLA3 antibody
    • Hypercholesterolemia autosomal dominant 3 antibody
    • LDLCQ1 antibody
    • NARC 1 antibody
    • NARC-1 antibody
    • NARC1 antibody
    • Neural apoptosis regulated convertase 1 antibody
    • Neural apoptosis-regulated convertase 1 antibody
    • PC 9 antibody
    • PC9 antibody
    • PCSK 9 antibody
    • PCSK9 antibody
    • PCSK9_HUMAN antibody
    • Proprotein convertase 9 antibody
    • Proprotein convertase PC9 antibody
    • Proprotein convertase subtilisin/kexin type 9 antibody
    • PSEC0052 antibody
    • Subtilisin/kexin like protease PC9 antibody
    • Subtilisin/kexin-like protease PC9 antibody
    see all

Images

  • All lanes : Anti-PCSK9 antibody (ab28770) at 0.2 µg/ml

    Lane 1 : untransfected McA cell lysate.
    Lane 2 : McA cell lysate transfected with wild type human PCSK9
    Lane 3 : McA cell lysate transfected with S127R human PCSK9

    Predicted band size: 74 kDa
    Observed band size: 60,73 kDa
    why is the actual band size different from the predicted?

  • ICC/IF image of ab28770 stained SHSY5Y cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28770, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemical analysis of paraffin-embedded Human kidney tissue, staining PCSK9 with ab28770 at 4 µg/ml. Staining was detected using HRP. Antigen retrieval was by heat mediation in TRIS/EDTA buffer (pH 9).
  • Anti-PCSK9 antibody (ab28770) at 0.3 µg/ml + Human Adipose lysate in RIPA buffer at 35 µg

    Developed using the ECL technique.

    Predicted band size: 74 kDa

References

This product has been referenced in:
  • Wang L  et al. Meganuclease targeting of PCSK9 in macaque liver leads to stable reduction in serum cholesterol. Nat Biotechnol 36:717-725 (2018). Read more (PubMed: 29985478) »
  • Liu M  et al. PCSK9 is not involved in the degradation of LDL receptors and BACE1 in the adult mouse brain. J Lipid Res 51:2611-8 (2010). WB . Read more (PubMed: 20453200) »
See all 5 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Transfected HepG2 cells)
Loading amount
40 µg
Specification
Transfected HepG2 cells
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jul 08 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Liver)
Loading amount
20000 cells
Specification
Liver
Gel Running Conditions
Reduced Denaturing
Blocking step
Blocking one as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jun 21 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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