Recombinant
RabMAb

Recombinant Anti-PCSK9 antibody [EPR17827-117] (ab185194)

Overview

  • Product name

    Anti-PCSK9 antibody [EPR17827-117]
    See all PCSK9 primary antibodies
  • Description

    Rabbit monoclonal [EPR17827-117] to PCSK9
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cyt, IPmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Recombinant fragment within Mouse PCSK9 aa 450 to the C-terminus. The exact sequence is proprietary.
    Database link: Q80W65

  • Positive control

    • WB: Mouse kidney, liver and placenta tissue lysates; Hepa1-6 whole cell lysate. ICC/IF: Hepa1-6 cells. Flow: Hepa1-6 cells. IP: Hepa1-6 whole cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab185194 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 78, 60 kDa (predicted molecular weight: 74 kDa).
ICC/IF 1/100.
Flow Cyt 1/500.
IP 1/30.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function

      May be implicated in the differentiation of cortical neurons and may play a role in cholesterol homeostasis.
    • Tissue specificity

      Expressed in neuro-epithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells.
    • Involvement in disease

      Defects in PCSK9 are the cause of familial hypercholesterolemia 3 (FH3) [MIM:603776]. FH3 inheritance is autosomal dominant.
    • Sequence similarities

      Belongs to the peptidase S8 family.
      Contains 1 peptidase S8 domain.
    • Post-translational
      modifications

      The soluble zymogen undergoes autocatalytic intramolecular processing in the endoplasmic reticulum, resulting in the cleavage of its propeptide that remains associated with the secreted enzyme.
    • Cellular localization

      Secreted.
    • Information by UniProt
    • Database links

    • Alternative names

      • Convertase subtilisin/kexin type 9 preproprotein antibody
      • FH3 antibody
      • HCHOLA3 antibody
      • Hypercholesterolemia autosomal dominant 3 antibody
      • LDLCQ1 antibody
      • NARC 1 antibody
      • NARC-1 antibody
      • NARC1 antibody
      • Neural apoptosis regulated convertase 1 antibody
      • Neural apoptosis-regulated convertase 1 antibody
      • PC 9 antibody
      • PC9 antibody
      • PCSK 9 antibody
      • PCSK9 antibody
      • PCSK9_HUMAN antibody
      • Proprotein convertase 9 antibody
      • Proprotein convertase PC9 antibody
      • Proprotein convertase subtilisin/kexin type 9 antibody
      • PSEC0052 antibody
      • Subtilisin/kexin like protease PC9 antibody
      • Subtilisin/kexin-like protease PC9 antibody
      see all

    Images

    • All lanes : Anti-PCSK9 antibody [EPR17827-117] (ab185194) at 1/1000 dilution

      Lane 1 : Mouse kidney tissue lysate
      Lane 2 : Mouse liver tissue lysate
      Lane 3 : Mouse placenta tissue lysate
      Lane 4 : Hepa1-6 (mouse hepatoma epithelial cell line) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 74 kDa
      Observed band size: 60 kDa
      why is the actual band size different from the predicted?



      Exposure time : Lanes 1-3:3 minutes; Lane 4:6 seconds.

      Blocking/diluting buffer and concentration: 5% NFDM/TBST

      The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 26092101).

    • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Hepa1-6 (mouse hepatoma epithelial cell line) cells labeling PCSK9 with ab185194 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in Hepa1-6 cell line is observed.

      The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is an Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    • Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized Hepa1-6 (mouse hepatoma epithelial cell line) cell line labeling PCSK9 with ab185194 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

    • PCSK9 was immunoprecipitated from 0.35 mg Hepa1-6 (mouse hepatoma epithelial cell line) whole cell lysate with ab185194 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab185194 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.

      Lane 1: Hepa1-6 whole cell lysate 10 µg (input).
      Lane 2: ab185194 IP in Hepa1-6 whole cell lysate (+).
      Lane 3: Rabbit monoclonal IgG in Hepa1-6 whole cell lsyate (-).

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.
      Exposure time: 5 seconds.

      78-kDa band represents proPCSK9 (PMID: 25210046).

       

    References

    ab185194 has not yet been referenced specifically in any publications.

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