Overview

  • Product name
    Anti-PCSK9 antibody [EPR7627(2)]
    See all PCSK9 primary antibodies
  • Description
    Rabbit monoclonal [EPR7627(2)] to PCSK9
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IP, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human PCSK9 aa 550-650. The exact sequence is proprietary.
    Database link: Q8NBP7

  • Positive control
    • WB: A431, BxPC-3 and HepG2 cell lysates and Human fetal liver tissue lysate. IP: HepG2 cell lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab181142 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/30 - 1/50.
WB 1/1000 - 1/10000. Detects a band of approximately 62, 78 kDa (predicted molecular weight: 74 kDa).

Target

  • Function
    May be implicated in the differentiation of cortical neurons and may play a role in cholesterol homeostasis.
  • Tissue specificity
    Expressed in neuro-epithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells.
  • Involvement in disease
    Defects in PCSK9 are the cause of familial hypercholesterolemia 3 (FH3) [MIM:603776]. FH3 inheritance is autosomal dominant.
  • Sequence similarities
    Belongs to the peptidase S8 family.
    Contains 1 peptidase S8 domain.
  • Post-translational
    modifications
    The soluble zymogen undergoes autocatalytic intramolecular processing in the endoplasmic reticulum, resulting in the cleavage of its propeptide that remains associated with the secreted enzyme.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Convertase subtilisin/kexin type 9 preproprotein antibody
    • FH3 antibody
    • HCHOLA3 antibody
    • Hypercholesterolemia autosomal dominant 3 antibody
    • LDLCQ1 antibody
    • NARC 1 antibody
    • NARC-1 antibody
    • NARC1 antibody
    • Neural apoptosis regulated convertase 1 antibody
    • Neural apoptosis-regulated convertase 1 antibody
    • PC 9 antibody
    • PC9 antibody
    • PCSK 9 antibody
    • PCSK9 antibody
    • PCSK9_HUMAN antibody
    • Proprotein convertase 9 antibody
    • Proprotein convertase PC9 antibody
    • Proprotein convertase subtilisin/kexin type 9 antibody
    • PSEC0052 antibody
    • Subtilisin/kexin like protease PC9 antibody
    • Subtilisin/kexin-like protease PC9 antibody
    see all

Images

  • Anti-PCSK9 antibody [EPR7627(2)] (ab181142) at 1/5000 dilution (purified) + BxPC-3 whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/5000 dilution

    Predicted band size: 74 kDa
    Observed band size: 62,78 kDa (why is the actual band size different from the predicted?)



    Blocking and dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-PCSK9 antibody [EPR7627(2)] (ab181142) at 1/20000 dilution (purified)

    Lane 1 : A431 whole cell lysate
    Lane 2 : HepG2 whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 74 kDa
    Observed band size: 62,78 kDa (why is the actual band size different from the predicted?)



    Blocking and dilution buffer: 5% NFDM/TBST.

  • ab181142 (purified) at 1/50 immunoprecipitating PCSK9 in HepG2 whole cell lysate.

    Lane 1 (input): HepG2 whole cell lysate (10µg)

    Lane 2 (+): ab181142 + HepG2 whole cell lysate.

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab181142 in HepG2 whole cell lysate.

    For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • All lanes : Anti-PCSK9 antibody [EPR7627(2)] (ab181142) at 1/10000 dilution (unpurified)

    Lane 1 : A431 (Human epidermoid carcinoma cell line) cell lysate
    Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 74 kDa
    Observed band size: 62,78 kDa (why is the actual band size different from the predicted?)

  • Anti-PCSK9 antibody [EPR7627(2)] (ab181142) at 1/2000 dilution (unpurified) + Human fetal liver lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 74 kDa
    Observed band size: 62 kDa (why is the actual band size different from the predicted?)

  • Western blot analysis of PCSK9 in HepG2 cell lysate immunoprecipitated using unpurified ab181142 at a dilution of 1/50.

    Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.

References

This product has been referenced in:
  • Xu X  et al. PCSK9 regulates apoptosis in human lung adenocarcinoma A549 cells via endoplasmic reticulum stress and mitochondrial signaling pathways. Exp Ther Med 13:1993-1999 (2017). Read more (PubMed: 28565798) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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