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    pctaire3-antibody-ab168442.pdf

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Signal Transduction Protein Phosphorylation Ser / Thr Kinases Other Kinases
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Anti-PCTAIRE3 antibody (ab168442)

  • Datasheet
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Abpromise

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Western blot - Anti-PCTAIRE3 antibody (ab168442)

    Key features and details

    • Mouse polyclonal to PCTAIRE3
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Secondary
    Product image
    Goat Anti-Mouse IgG H&L (HRP) (ab205719)

    View more associated products

    Overview

    • Product name

      Anti-PCTAIRE3 antibody
      See all PCTAIRE3 primary antibodies
    • Description

      Mouse polyclonal to PCTAIRE3
    • Host species

      Mouse
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse, Rat, Cow, Rhesus monkey, Orangutan
    • Immunogen

      Full length protein, corresponding to amino acids 1-474 of Human PCTAIRE3 (NP_002587.2).

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • Positive control

      • PCTAIRE3 transfected 293T cell line lysate
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
    • Storage buffer

      pH: 7.4
      Constituent: 99% PBS
    • Concentration information loading...
    • Purity

      Protein A purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Signal Transduction
      • Protein Phosphorylation
      • Ser / Thr Kinases
      • Other Kinases

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG - Isotype Control (ab37355)
    • Recombinant Protein

      • Recombinant Human PCTAIRE3 protein (ab179959)

    Applications

    Our Abpromise guarantee covers the use of ab168442 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB Use a concentration of 1 µg/ml. Predicted molecular weight: 54 kDa.

    Target

    • Function

      May play a role in signal transduction cascades in terminally differentiated cells.
    • Tissue specificity

      Isoform 3 expression is limited to several subcortical nuclei of the basal gangli and the spinal cord. Isoform 2 is widely expressed.
    • Sequence similarities

      Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
      Contains 1 protein kinase domain.
    • Target information above from: UniProt accession Q07002 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 5129 Human
      • Entrez Gene: 18557 Mouse
      • Entrez Gene: 289019 Rat
      • Omim: 169190 Human
      • SwissProt: Q07002 Human
      • SwissProt: Q04899 Mouse
      • SwissProt: O35832 Rat
      • Unigene: 445402 Human
      • Unigene: 28130 Mouse
      • Unigene: 219420 Rat
      see all
    • Alternative names

      • Cdk18 antibody
      • CDK18_HUMAN antibody
      • Cell division protein kinase 18 antibody
      • Cyclin-dependent kinase 18 antibody
      • PCTAIRE 3 antibody
      • PCTAIRE antibody
      • PCTAIRE motif protein kinase 3 antibody
      • PCTAIRE protein kinase 3 antibody
      • PCTAIRE-motif protein kinase 3 antibody
      • PCTK 3 antibody
      • Serine/threonine-protein kinase PCTAIRE-3 antibody
      see all

    Images

    • Western blot - Anti-PCTAIRE3 antibody (ab168442)
      Western blot - Anti-PCTAIRE3 antibody (ab168442)
      All lanes : Anti-PCTAIRE3 antibody (ab168442) at 1 µg/ml

      Lane 1 : PCTAIRE3 transfected 293T cell line lysate
      Lane 2 : Non-transfected 293T cell line lysate


      Lysates/proteins at 15 µl per lane.

      Secondary
      All lanes : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

      Developed using the ECL technique.

      Predicted band size: 54 kDa

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab168442? Please let us know so that we can cite the reference in this datasheet.

    ab168442 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Western blot abreview for Anti-PCTAIRE3 antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (brain)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    brain
    Blocking step
    Milk as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 10 2017

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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