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Rabbit monoclonal [EPR15390] to PCTP
Synthetic peptide within Human PCTP aa 50-150. The exact sequence is proprietary.
Database link: Q9UKL6
Human fetal liver and K562 cell lysates, K562 cells.
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Protein A purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/30 - 1/50.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
Catalyzes the transfer of phosphatidylcholine between membranes. Binds a single lipid molecule.
Highest expression in liver, placenta, testis, kidney and heart. Low levels in brain and lung. No expression detected in thymus.
Contains 1 START domain.
Information by UniProt
PC TP antibody
Western blot - Anti-PCTP antibody [EPR15390] (ab184944)
All lanes : Anti-PCTP antibody [EPR15390] (ab184944) at 1/1000 dilution Lane 1 : Human fetal liver lysate Lane 2 : K562 cell lysate Lysates/proteins at 20 µg per lane. Secondary All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution Developed using the ECL technique. Predicted band size: 25 kDa Observed band size: 25 kDa
Flow Cytometry - Anti-PCTP antibody [EPR15390] (ab184944)
Flow cytometry analysis of paraformaldehyde-fixed K562 cells labeling PCTP with ab184944 at a 1/200 dilution (Red), with a negative control rabbit IgG (Green). Secondary antibody goat anti-rabbit IgG (FITC) at a 1/150 dilution.
Immunoprecipitation - Anti-PCTP antibody [EPR15390] (ab184944)
Western blot analysis on immunoprecipitation from Human fetal liver lysate (Lane 1), labeling PCTP using ab184944 at 1/50 dilution Lane 2 shows PBS negative control. Secondary antibody, HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG at a 1/1500 dilution.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"