Overview

  • Product name

  • Description

    Rabbit polyclonal to PCYT2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Cow, Chimpanzee, Macaque monkey, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human PCYT2 aa 150-250 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab157389)

  • Positive control

    • This antibody gave a positive signal in Human Liver tissue lysates as well as HepG2 whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab135290 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).

Target

  • Function

    Plays an important role in the biosynthesis of the phospholipid phosphatidylethanolamine. Catalyzes the formation of CDP-ethanolamine.
  • Tissue specificity

    Strongest expression in liver, heart, and skeletal muscle.
  • Pathway

    Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from ethanolamine: step 2/3.
  • Sequence similarities

    Belongs to the cytidylyltransferase family.
  • Information by UniProt
  • Database links

  • Alternative names

    • CTP:phosphoethanolamine cytidylyltransferase antibody
    • ET antibody
    • Ethanolamine-phosphate cytidylyltransferase antibody
    • Ethanolaminephosphate Cytidyltransferase antibody
    • PCY2 antibody
    • PCY2_HUMAN antibody
    • Pcyt2 antibody
    • Phosphate cytidylyltransferase 2 ethanolamine antibody
    • Phosphorylethanolamine transferase antibody
    see all

Images

  • All lanes : Anti-PCYT2 antibody (ab135290) at 1/250 dilution

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 44 kDa
    Observed band size: 44 kDa
    Additional bands at: 16 kDa, 47 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 90 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab135290 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References

ab135290 has not yet been referenced specifically in any publications.

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