Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PD-L1 antibody [28-8]

Inconclusive
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (lymph node)
Antigen retrieval step
Heat mediated
Permeabilization
No
Specification
lymph node
Blocking step
ultratek kit, ScyTek Laboratories, Logan, Utah, USA as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 20°C
Fixative
Formaldehyde

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: emerald diluent antibody, Cellmarque, Si
Dilution
1/100

Secondary antibody

Name
Non-Abcam antibody was used: Ultratek HRP kit, ScyTek Laboratories, Logan, Utah
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin

Additional data

Additional Notes
The antibody has been tested at the preconized dilution : 1 :500 with an heating antigen retrieval for 20 minutes at pH6 and pH9 and no labelling was observed. Antoher tes was performed, at a dilution of 1:100, without antigen retrieval and with an heating antigen retrieval for 40 minutes at pH 4, pH6 and pH9: no labelling was observed. With the tested conditions the antibody does not seem to work on canine tissue.
Abcam response
We would like to thank the reviewer for submitting this AbReview for ab205921. As the product had not yet been tested in this species we were unsure what the result might be and we appreciate the time and effort that the reviewer has put in to testing this product in this species. We encourage all our customers to contact us should they wish to test any of our products in untested species or applications, as these may qualify for our AbTrial program.

Sara Belluco

Verified customer

Submitted Sep 12 2017

Sign up