Product nameAnti-PD-L1 antibody [28-8] (FITC)
See all PD-L1 primary antibodies
DescriptionRabbit monoclonal [28-8] to PD-L1 (FITC)
ConjugationFITC. Ex: 493nm, Em: 528nm
Tested applicationsSuitable for: ICC/IF, Flow Cytmore details
Species reactivityReacts with: Human
Recombinant full length protein corresponding to Human PD-L1 (extracellular). The immunogen contains the specific extracellular domain of huPD-L1 (Phe19-Thr239). See reference for more info - www.ncbi.nlm.nih.gov/pmc/articles/PMC4561627/
Database link: Q9NZQ7
- Flow Cyt: CHO-PDL1 cells. ICC/IF: CHO-PDL1 cells
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.
Storage bufferPreservative: 0.02% Sodium azide
Constituents: 30% Glycerol, 1% BSA, PBS
Concentration information loading...
PurityImmunogen affinity purified
- Anti-PD-L1 antibody [28-8] (ab205921)
- Anti-PD-L1 antibody [28-8] (Allophycocyanin) (ab206967)
- Anti-PD-L1 antibody [28-8] - Low endotoxin, Azide free (ab209889)
- Anti-PD-L1 antibody [28-8] (Alexa Fluor® 488) (ab209959)
- Anti-PD-L1 antibody [28-8] (Alexa Fluor® 647) (ab209960)
- Anti-PD-L1 antibody [28-8] (HRP) (ab209961)
- Anti-PD-L1 antibody [28-8] (Phycoerythrin) (ab209962)
- Anti-PD-L1 antibody [28-8] (Alexa Fluor® 555) (ab213358)
- Anti-PD-L1 antibody [28-8] (Alexa Fluor® 568) (ab213359)
- Anti-PD-L1 antibody [28-8] (Alexa Fluor® 594) (ab213360)
- Human PD-L1 ELISA Kit [28-8] (ab214565)
Our Abpromise guarantee covers the use of ab224027 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This product gave a positive signal in CHO-PDL1 cells fixed with 4% formaldehyde (10 min)
FunctionInvolved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production.
Tissue specificityHighly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes.
Sequence similaritiesBelongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Cellular localizationCell membrane and Endomembrane system.
- Information by UniProt
- B7 H antibody
- B7 H1 antibody
- B7 homolog 1 antibody
ab224027 staining PDL1 in CHO-PDL1 cells. The lower panels demonstrate that ab224027 does not cross react with un-transfected CHO-S cells.
The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab224027 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Overlay histogram showing CHO-S (blue line) and CHO-PD-L1 transfected (red line) cells stained with ab224027.
The cells were incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab224027, 1/500 dilution) for 30 min at 22ºC.
Acquisition of >5,000 events were collected using a 20 mW solid-state laser (488nm) and 530/30 bandpass filter.
ab224027 has not yet been referenced specifically in any publications.