PD-L1 antibody (28-8) IHC antigen retrieval and detection panel (ab236676)

Overview

  • Product name
    PD-L1 antibody (28-8) IHC antigen retrieval and detection panel
    See all PD-L1 kits
  • Species reactivity
    Reacts with: Human
  • Product overview

    PD-L1 antibody (28-8) IHC antigen retrieval and detection kit ab236676 contains the 28-8 clone PD-L1 antibody (ab205921), and the critical reagents we recommend using with it for immunohistochemistry on paraffin-embedded tissue sections.


    Kit contains:


    ab205921 PD-L1 antibody (28-8)


    ab208572 HIER antigen retrieval reagent


    ab209101 Rabbit specific IHC polymer detection kit HRP


    ab209101 contains amplifier secondary antibody, and HRP polymer tertiary antibody. 


    For instructions for use please consult the protocol books found on the online datasheets for ab205921, ab208572, and ab209101.

  • Notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

  • Tested applications
    Suitable for: IHC-P, IHC-Pmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab236676 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human non-small cell lung cancer tissue labelling PD-L1 with ab205921. Tumor cells and immuno cells localized within the stroma show PD-L1 plasma membrane staining.

    For antigen retrival buffer, Universal HIER antigen retrieval reagent (ab208572) was used.
    For IHC detection kit, Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) is recommended.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human melanoma tissue labelling PD-L1 with ab205921 on Ventana Ultra. Tumor cells and immune cells show PD-L1 positive plasma membrane staining.

    For antigen retrival buffer, Universal HIER antigen retrieval reagent (ab208572) was used.
    For IHC detection kit, Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) is recommended.

Protocols

References

ab236676 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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