Key features and details
- Mouse monoclonal [29E.2A3] to PD-L1 (PerCP/Cy5.5®)
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PerCP/Cy5.5®. Ex: 482nm, Em: 690nm
- Isotype: IgG2a
Product nameAnti-PD-L1 antibody [29E.2A3] (PerCP/Cy5.5®)
See all PD-L1 primary antibodies
DescriptionMouse monoclonal [29E.2A3] to PD-L1 (PerCP/Cy5.5®)
ConjugationPerCP/Cy5.5®. Ex: 482nm, Em: 690nm
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Human
Full length native protein (purified) corresponding to Human PD-L1.
Database link: Q9NZQ7
- Flow cyt: peripheral blood mononuclear cells.
This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at +4°C. Do Not Freeze.
Storage bufferpH: 7.4
Preservative: 0.1% Sodium azide
Concentration information loading...
Purification notesPurified from TCS.
Light chain typekappa
Our Abpromise guarantee covers the use of ab272349 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 4µg for 10 cells.|
FunctionInvolved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production.
Tissue specificityHighly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes.
Sequence similaritiesBelongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Cellular localizationCell membrane and Endomembrane system.
- Information by UniProt
- B7 H antibody
- B7 H1 antibody
- B7 homolog 1 antibody
Separation of human monocytes (Red) from cellular debris (black-dashed) in flow cytometric analysis labeling CD274 using ab272349. (4 μl reagent per milion cells in 100 μl of cell suspension).
Flow cytometric analysis of peripheral blood mononuclear cells labeling CD274 with ab272349 at 4μl reagent per milion cells in 100 μl of cell suspension. (4 μl reagent per milion cells in 100 μl of cell suspension).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab272349 has not yet been referenced specifically in any publications.