Key features and details
- Rabbit polyclonal to PD-L1
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human, Pig
- Isotype: IgG
Product nameAnti-PD-L1 antibody
See all PD-L1 primary antibodies
DescriptionRabbit polyclonal to PD-L1
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human, Pig
Recombinant fragment (His-tag) corresponding to Human PD-L1 aa 24-234. (Expressed in E.coli).
PKDLYVVEYGSNMTIECKFPVEKQLDLAALIVYWEMEDKNIIQFVHGEED LKVQHSSYRQRARLLKDQLSLGNAALQITDVKLQDAGVYRCMISYGGADY KRITVKVNAPYNKINQRILVVDPVTSEHELTCQAEGYPKAEVIWTSSDHQ VLSGKTTTTNSKREEKLFNVTSTLRINTTTNEIFYCTFRRLDPEENHTAE LVIPELPLAHP
Database link: Q9NZQ7
- WB: Mouse heart lysate; Pig heart and skeletal muscle lysate; Recombinant human PD-L1 protein. IHC-P: Human tonsil tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab233482 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 20 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Treating samples with PNGase F may increase signal intensity.
Positive Control: Hu tonsil, placenta tissues, head and neck squamous cell carcinoma
Negative Control: MCF-7
|WB||Use a concentration of 0.5 - 2 µg/ml.
Treat samples with PNGase F to confirm the specificity of bands if necessary. PMID: 31327656
The observed band size is not exactly as predicted MWs in WB due to the different forms and modifications of PD-L1.
Hu Isoform I-3: 20-33 kDa (predicted)
Ms: 32.8 kDa (predicted)
Actual band size in WB is between 40-60 kDa.
Positive Control: B-CPAP, MD-MB-231, U87-MG
Negative Control: SW480
FunctionInvolved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production.
Tissue specificityHighly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes.
Sequence similaritiesBelongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Cellular localizationCell membrane and Endomembrane system.
- Information by UniProt
- B7 H antibody
- B7 H1 antibody
- B7 homolog 1 antibody
Formalin-fixed, paraffin-embedded human tonsil tissue stained for PD-L1 using ab233482 at 10 µg/ml in immunohistochemical analysis. DAB staining.
All lanes : Anti-PD-L1 antibody (ab233482) at 2 µg/ml
Lane 1 : Mouse heart lysate
Lane 2 : Rat heart lysate
Anti-PD-L1 antibody (ab233482) at 2 µg/ml + Pig skeletal muscle lysate
Anti-PD-L1 antibody (ab233482) at 2 µg/ml + Pig heart lysate
Anti-PD-L1 antibody (ab233482) at 2 µg/ml + Recombinant human PD-L1 protein
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab233482 has been referenced in 1 publication.
- Nambiar DK et al. Galectin-1-driven T cell exclusion in the tumor endothelium promotes immunotherapy resistance. J Clin Invest 129:5553-5567 (2019). PubMed: 31710313