Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BLR020E] to PD-L1
- Suitable for: WB, ICC/IF, IHC-P, IP
- Reacts with: Human
Product nameAnti-PD-L1 antibody [BLR020E]
See all PD-L1 primary antibodies
DescriptionRabbit monoclonal [BLR020E] to PD-L1
Tested applicationsSuitable for: WB, ICC/IF, IHC-P, IPmore details
Species reactivityReacts with: Human
Synthetic peptide within Human PD-L1 aa 250-290 (C terminal). The exact sequence is proprietary. NP_054862.1 and Gene ID 29126.
Database link: Q9NZQ7
This product is sold under License from Bethyl Laboratories, Inc.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA
Concentration information loading...
Purification notesRecombinant antibody was purified from cell culture supernatant.
Our Abpromise guarantee covers the use of ab243877 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Treat samples with PNGase F to confirm the specificity of bands if necessary. PMID: 31327656
The observed band size is not exactly as predicted MWs in WB due to the different forms and modifications of PD-L1.
Hu Isoform I-3: 20-33 kDa (predicted)
Actual band size in WB is between 40-60 kDa.
Positive Control: B-CPAP, MD-MB-231, U87-MG
Negative Control: A549, SW480
|ICC/IF||1/100 - 1/500.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Treating samples with PNGase F may increase signal intensity.
Positive Control: Hu tonsil, placenta tissues, head and neck squamous cell carcinoma
Negative Control: MCF-7
|IP||Use at an assay dependent concentration.
Use 20µl/mg lysate.
FunctionInvolved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production.
Tissue specificityHighly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes.
Sequence similaritiesBelongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Cellular localizationCell membrane and Endomembrane system.
- Information by UniProt
- B7 H antibody
- B7 H1 antibody
- B7 homolog 1 antibody
PDL-1 was immunoprecipitated from SUP-M2 whole cell lysate with ab243877. Western blot was performed on the immunoprecipitate using ab243877 at 1/1000 dilution.
Lane 1: ab243877 IP in SUP-M2 whole cell lysate.
Lane 2: Contol IgG in SUP-M2 whole cell lysate.
Western blot analysis using ab243877 at 1/1000 dilution.
Lane 1: SUP-M2 whole cell lysate (50 µg).
Lane 2: HDLM-2 whole cell lysate (50 µg).
Lane 3: HEK-293T whole cell lysate (50 µg).
Lane 4: Ramos whole cell lysate (50 µg).
Lane 5: Jurkat whole cell lysate (50 µg).
Lane 6: PD-L2 whole cell lysate (50 µg).
Lane 7: PD-L1 whole cell lysate (50 µg).
Lane 8: HEK-293 whole cell lysate (50 µg).
A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence.
Formaldehyde-fixed HDML-2 cells labeling PDL-1 using ab243876 at 1/250 dilution in ICC/IF analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary antibody. DAB staining
Formalin-fixed, paraffin-embedded human tonsil tissue stained for PD-L1 using ab243877 at 1/100 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.
Formalin-fixed, paraffin-embedded human lung carcinoma tissue stained for PD-L1 using ab243877 at 1/100 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.
Formalin-fixed, paraffin-embedded human lung carcinoma tissue stained for PD-L1 using ab243877 at 1/100 dilution in immunohistochemical analysis. A DyLight𪨂-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab243877 has not yet been referenced specifically in any publications.