Overview

  • Product name

    Anti-PD-L1 antibody [BLR020E]
    See all PD-L1 primary antibodies
  • Description

    Rabbit monoclonal [BLR020E] to PD-L1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-P, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human PD-L1 aa 250-290 (C terminal). The exact sequence is proprietary. NP_054862.1 and Gene ID 29126.
    Database link: Q9NZQ7

  • General notes

    This product is sold under License from Bethyl Laboratories, Inc.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab243877 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000.
ICC/IF 1/100 - 1/500.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at an assay dependent concentration.

Use 20µl/mg lysate.

Target

  • Function

    Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production.
  • Tissue specificity

    Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes.
  • Sequence similarities

    Belongs to the immunoglobulin superfamily. BTN/MOG family.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization

    Cell membrane and Endomembrane system.
  • Information by UniProt
  • Database links

  • Alternative names

    • B7 H antibody
    • B7 H1 antibody
    • B7 homolog 1 antibody
    • B7-H1 antibody
    • B7H antibody
    • B7H1 antibody
    • CD 274 antibody
    • CD274 antibody
    • CD274 antigen antibody
    • CD274 molecule antibody
    • MGC142294 antibody
    • MGC142296 antibody
    • OTTHUMP00000021029 antibody
    • PD L1 antibody
    • PD-L1 antibody
    • PD1L1_HUMAN antibody
    • PDCD1 ligand 1 antibody
    • PDCD1L1 antibody
    • PDCD1LG1 antibody
    • PDL 1 antibody
    • PDL1 antibody
    • Programmed cell death 1 ligand 1 antibody
    • Programmed death ligand 1 antibody
    • RGD1566211 antibody
    see all

Images

  • PDL-1 was immunoprecipitated from SUP-M2 whole cell lysate with ab243877. Western blot was performed on the immunoprecipitate using ab243877 at 1/1000 dilution.

    Lane 1: ab243877 IP in SUP-M2 whole cell lysate.

    Lane 2: Contol IgG in SUP-M2 whole cell lysate.

    Detection: Chemiluminescence.

  • Western blot analysis using ab243877 at 1/1000 dilution.

    Lane 1: SUP-M2 whole cell lysate (50 µg).

    Lane 2: HDLM-2 whole cell lysate (50 µg).

    Lane 3: HEK-293T whole cell lysate (50 µg).

    Lane 4: Ramos whole cell lysate (50 µg).

    Lane 5: Jurkat whole cell lysate (50 µg).

    Lane 6: PD-L2 whole cell lysate (50 µg).

    Lane 7: PD-L1 whole cell lysate (50 µg).

    Lane 8: HEK-293 whole cell lysate (50 µg).

    A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence.

  • Formaldehyde-fixed HDML-2 cells labeling PDL-1 using ab243876 at 1/250 dilution in ICC/IF analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary antibody. DAB staining
  • Formalin-fixed, paraffin-embedded human tonsil tissue stained for PD-L1 using ab243877 at 1/100 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.
  • Formalin-fixed, paraffin-embedded human lung carcinoma tissue stained for PD-L1 using ab243877 at 1/100 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.
  • Formalin-fixed, paraffin-embedded human lung carcinoma tissue stained for PD-L1 using ab243877 at 1/100 dilution in immunohistochemical analysis. A DyLight𪨂-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.

References

ab243877 has not yet been referenced specifically in any publications.

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