Product nameAnti-PD-L1 antibody [EPR19759] (HRP)
See all PD-L1 primary antibodies
DescriptionRabbit monoclonal [EPR19759] to PD-L1 (HRP)
Tested applicationsSuitable for: WBmore details
Predicted to work with: Human
Synthetic peptide. within Human PD-L1 aa 250 to the C-terminus. The exact sequence is proprietary.
Database link: Q9NZQ7
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.1% CMIT/MIT 3:1
Constituents: 1% BSA, 30% Glycerol, PBS
Concentration information loading...
PurityProtein A purified
- Anti-PD-L1 antibody [EPR19759] (ab213524)
- Anti-PD-L1 antibody [EPR19759] (Alexa Fluor® 488) (ab214958)
- Anti-PD-L1 antibody [EPR19759] (Alexa Fluor® 647) (ab215251)
- Anti-PD-L1 antibody [EPR19759] (Alexa Fluor® 594) (ab216742)
- Anti-PD-L1 antibody [EPR19759] (Alexa Fluor® 568) (ab220314)
- Anti-PD-L1 antibody [EPR19759] - BSA and Azide free (ab221612)
- Anti-PD-L1 antibody [EPR19759] - Low endotoxin, Azide free (ab246695)
Our Abpromise guarantee covers the use of ab215349 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Detects a band of approximately 40-52 kDa (predicted molecular weight: 33 kDa).|
FunctionInvolved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production.
Tissue specificityHighly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes.
Sequence similaritiesBelongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Cellular localizationCell membrane and Endomembrane system.
- Information by UniProt
- B7 H antibody
- B7 H1 antibody
- B7 homolog 1 antibody
All lanes : Anti-PD-L1 antibody [EPR19759] (HRP) (ab215349) at 1/5000 dilution
Lane 1 : CHO-S
Lane 2 : CHO-PDL1
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 40-52 kDa why is the actual band size different from the predicted?
Exposure time: 8 minutes
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab215349 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
ab215349 has not yet been referenced specifically in any publications.