Overview

  • Product name

    Anti-PD-L1 antibody [PDL1/2746]
    See all PD-L1 primary antibodies
  • Description

    Mouse monoclonal [PDL1/2746] to PD-L1
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, WB, Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to Human PD-L1 aa 39-191.
    Database link: Q9NZQ7

  • Positive control

    • IHC-P: Human lung SCC, cervical carcinoma, breast carcinoma and basal cell carcinoma tissue. FC: Jurkat cells. ICC/IF: Jurkat cells. WB: HEK-293, RAW 264.7, A549, THP-1, MOLT-4, Raji, MDA-MB-231 and HepG2 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab238697 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 - 2 µg/ml.

Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Incubate with primary antibody for 30 minutes at RT.

WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 33 kDa.
Flow Cyt Use 1-2µg for 106 cells.
ICC/IF Use a concentration of 1 - 2 µg/ml.

Target

  • Function

    Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production.
  • Tissue specificity

    Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes.
  • Sequence similarities

    Belongs to the immunoglobulin superfamily. BTN/MOG family.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization

    Cell membrane and Endomembrane system.
  • Information by UniProt
  • Database links

  • Alternative names

    • B7 H antibody
    • B7 H1 antibody
    • B7 homolog 1 antibody
    • B7-H1 antibody
    • B7H antibody
    • B7H1 antibody
    • CD 274 antibody
    • CD274 antibody
    • CD274 antigen antibody
    • CD274 molecule antibody
    • MGC142294 antibody
    • MGC142296 antibody
    • OTTHUMP00000021029 antibody
    • PD L1 antibody
    • PD-L1 antibody
    • PD1L1_HUMAN antibody
    • PDCD1 ligand 1 antibody
    • PDCD1L1 antibody
    • PDCD1LG1 antibody
    • PDL 1 antibody
    • PDL1 antibody
    • Programmed cell death 1 ligand 1 antibody
    • Programmed death ligand 1 antibody
    • RGD1566211 antibody
    see all

Images

  • Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for PD-L1 with ab238697 at 2 µg/ml in immunohistochemical analysis.

  • All lanes : Anti-PD-L1 antibody [PDL1/2746] (ab238697) at 2 µg/ml

    Lane 1 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cell lysate
    Lane 2 : A549 (human lung carcinoma cell line) cell lysate
    Lane 3 : THP-1 (human monocytic leukemia cell line) cell lysate
    Lane 4 : MOLT-4 (human lymphoblastic leukemia cell line) cell lysate
    Lane 5 : Raji (human Burkitt's lymphoma cell line) cell lysate
    Lane 6 : MDA-MB-231 (human breast adenocarcinoma cell line) cell lysate

    Predicted band size: 33 kDa

  • Flow Cytometry analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cells, labeling PD-L1 with ab238697, followed by Goat anti-mouse IgG-CF488 (orange) compared to cells alone (blue) and isotype control (red).

  • Analysis of Protein Array containing more than 19,000 full-length human proteins using ab238697.

  • Jurkat (human T cell leukemia cell line from peripheral blood) cells stained for PD-L1 using ab238697 at 2 µg/ml dilution in ICC/IF, followed by Goat anti-mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).

  • Formalin-fixed, paraffin-embedded human basal cell carcinoma tissue stained for PD-L1 with ab238697 at 2 µg/ml in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human cervical carcinoma tissue stained for PD-L1 with ab238697 at 2 µg/ml in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human lung SCC tissue stained for PD-L1 with ab238697 at 2 µg/ml in immunohistochemical analysis.

  • All lanes : Anti-PD-L1 antibody [PDL1/2746] (ab238697) at 2 µg/ml

    Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
    Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate

    Predicted band size: 33 kDa

References

ab238697 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab238697.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up