Anti-PD-L1 antibody [SP142] - C-terminal (ab228462)
- Datasheet
- Specific References (56)
- Protocols
Overview
-
Product nameAnti-PD-L1 antibody [SP142] - C-terminal
See all PD-L1 primary antibodies -
DescriptionRabbit monoclonal [SP142] to PD-L1 - C-terminal
-
Host speciesRabbit
-
Tested applicationsSuitable for: IHC-Pmore details
-
Species reactivityReacts with: Human
-
Immunogen
Synthetic peptide within Human PD-L1 aa 250 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: Q9NZQ7 -
Positive control
- IHC-P: Human placenta, tonsil, lung squamous cell carcinoma, cervical squamous cell carcinoma, skin squamous cell carcinoma, Hodgkin's lymphoma, pancreatic adenocarcinoma and prostate adenocarcinoma tissues.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
-
Storage bufferpH: 7.6
Preservative: 0.099% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
PurityImmunogen affinity purified
-
Purification notesPurified from TCS by protein A/G.
-
ClonalityMonoclonal
-
Clone numberSP142
-
IsotypeIgG
-
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab228462 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100. Boil tissue section in EDTA buffer, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes. Primary antibody incubation for 10 minutes at room temperature. |
Target
-
FunctionInvolved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production.
-
Tissue specificityHighly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes.
-
Sequence similaritiesBelongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localizationCell membrane and Endomembrane system.
- Information by UniProt
-
Database links
- Entrez Gene: 29126 Human
- Omim: 605402 Human
- SwissProt: Q9NZQ7 Human
- Unigene: 521989 Human
-
Alternative names
- B7 H antibody
- B7 H1 antibody
- B7 homolog 1 antibody
see all
Images
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [SP142] - C-terminal (ab228462)](https://www.abcam.com/ps/products/228/ab228462/Images/ab228462-293349-ab228462ihc1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [SP142] - C-terminal (ab228462)Formalin-fixed, paraffin-embedded human placenta tissue stained for PD-L1 using ab228462 at 1/100 dilution in immunohistochemical analysis.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [SP142] - C-terminal (ab228462)](https://www.abcam.com/ps/products/228/ab228462/Images/ab228462-293348-ab228462ihc2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [SP142] - C-terminal (ab228462)Formalin-fixed, paraffin-embedded human tonsil tissue stained for PD-L1 using ab228462 at 1/100 dilution in immunohistochemical analysis.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [SP142] - C-terminal (ab228462)](https://www.abcam.com/ps/products/228/ab228462/Images/ab228462-293347-ab228462ihc3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [SP142] - C-terminal (ab228462)Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for PD-L1 using ab228462 at 1/100 dilution in immunohistochemical analysis.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [SP142] - C-terminal (ab228462)](https://www.abcam.com/ps/products/228/ab228462/Images/ab228462-293346-ab228462ihc4.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [SP142] - C-terminal (ab228462)Formalin-fixed, paraffin-embedded human Hodgkin's lymphoma tissue stained for PD-L1 using ab228462 at 1/100 dilution in immunohistochemical analysis.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [SP142] - C-terminal (ab228462)](https://www.abcam.com/ps/products/228/ab228462/Images/ab228462-293345-ab228462ihc5.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [SP142] - C-terminal (ab228462)Formalin-fixed, paraffin-embedded human cervical squamous cell carcinoma tissue stained for PD-L1 using ab228462 at 1/100 dilution in immunohistochemical analysis.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [SP142] - C-terminal (ab228462)](https://www.abcam.com/ps/products/228/ab228462/Images/ab228462-293344-ab228462ihc6.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [SP142] - C-terminal (ab228462)Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for PD-L1 using ab228462 at 1/100 dilution in immunohistochemical analysis.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [SP142] - C-terminal (ab228462)](https://www.abcam.com/ps/products/228/ab228462/Images/ab228462-293343-ab228462ihc7.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [SP142] - C-terminal (ab228462)Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for PD-L1 using ab228462 at 1/100 dilution in immunohistochemical analysis.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [SP142] - C-terminal (ab228462)](https://www.abcam.com/ps/products/228/ab228462/Images/ab228462-293342-ab228462ihc8.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [SP142] - C-terminal (ab228462)Formalin-fixed, paraffin-embedded human skin squamous cell carcinoma tissue stained for PD-L1 using ab228462 at 1/100 dilution in immunohistochemical analysis.
Protocols
References
This product has been referenced in:
- Tomioka N et al. The therapeutic candidate for immune checkpoint inhibitors elucidated by the status of tumor-infiltrating lymphocytes (TILs) and programmed death ligand 1 (PD-L1) expression in triple negative breast cancer (TNBC). Breast Cancer 25:34-42 (2018). Read more (PubMed: 28488168) »
- Hirai A et al. Prognostic impact of programmed death-ligand 1 expression in correlation with human leukocyte antigen class I expression status in stage I adenocarcinoma of the lung. J Thorac Cardiovasc Surg 155:382-392.e1 (2018). Read more (PubMed: 28711324) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"