Recombinant
RabMAb

Recombinant Anti-PD-L1 antibody [SP142] - C-terminal, prediluted (ab228463)

Overview

  • Product name

    Anti-PD-L1 antibody [SP142] - C-terminal, prediluted
    See all PD-L1 primary antibodies
  • Description

    Rabbit monoclonal [SP142] to PD-L1 - C-terminal, prediluted
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human PD-L1 aa 250 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: Q9NZQ7

  • Positive control

    • IHC-P: Human placenta, tonsil, lung squamous cell carcinoma, cervical squamous cell carcinoma, skin squamous cell carcinoma, Hodgkin's lymphoma, pancreatic adenocarcinoma and prostate adenocarcinoma tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab228463 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1.

Ready to use.

Boil tissue section in EDTA buffer, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes.

Primary antibody incubation for 10 minutes at room temperature.

Target

  • Function

    Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production.
  • Tissue specificity

    Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes.
  • Sequence similarities

    Belongs to the immunoglobulin superfamily. BTN/MOG family.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization

    Cell membrane and Endomembrane system.
  • Information by UniProt
  • Database links

  • Alternative names

    • B7 H antibody
    • B7 H1 antibody
    • B7 homolog 1 antibody
    • B7-H1 antibody
    • B7H antibody
    • B7H1 antibody
    • CD 274 antibody
    • CD274 antibody
    • CD274 antigen antibody
    • CD274 molecule antibody
    • MGC142294 antibody
    • MGC142296 antibody
    • OTTHUMP00000021029 antibody
    • PD L1 antibody
    • PD-L1 antibody
    • PD1L1_HUMAN antibody
    • PDCD1 ligand 1 antibody
    • PDCD1L1 antibody
    • PDCD1LG1 antibody
    • PDL 1 antibody
    • PDL1 antibody
    • Programmed cell death 1 ligand 1 antibody
    • Programmed death ligand 1 antibody
    • RGD1566211 antibody
    see all

Images

  • IHC image of ab228462 staining PD-L1 in human tonsil formalin fixed paraffin embedded tissue sections*, performed on a Leica BOND RX (standard Protocol F, Polymer Refine kit). The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH9, epitope retrieval solution 2) for 30 mins at 98°C. The section was then incubated with ab228462, 1/400 working dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system for 8 minutes at room temperature. DAB was used as the chromogen for 10 minutes at room temperature. The section was then counterstained with hematoxylin, blued, dehydrated, cleared and mounted with DPX.

     

    This image was generated using ab228462, the same antibody but with BSA and Azide 

  • IHC image of ab228462 staining PD-L1 in PD-L1 Dynamic Range Analyte Control formalin fixed paraffin embedded cell lines (HistoCyte Laboratories), performed on a Leica BOND RX (standard Protocol F, Polymer Refine kit). The section was pre-treated using heat mediated antigen retrieval with EDTA  buffer (pH9, epitope retrieval solution 2) for 30 mins at 98°C. The section was then incubated with ab228462, 1/400 working dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system for 8 minutes at room temperature. DAB was used as the chromogen for 10 minutes at room temperature. The section was then counterstained with hematoxylin, blued, dehydrated, cleared and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    This image was generated using ab228462, the same antibody but with BSA and Azide 

  • Formalin-fixed, paraffin-embedded human placenta tissue stained for PD-L1 using ab228463 in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human tonsil tissue stained for PD-L1 using ab228463 in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for PD-L1 using ab228463 in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human Hodgkin's lymphoma tissue stained for PD-L1 using ab228463 in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human cervical squamous cell carcinoma tissue stained for PD-L1 using ab228463 in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for PD-L1 using ab228463 in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for PD-L1 using ab228463 in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human skin squamous cell carcinoma tissue stained for PD-L1 using ab228463 in immunohistochemical analysis.

References

This product has been referenced in:

  • Zacharakis N  et al. Immune recognition of somatic mutations leading to complete durable regression in metastatic breast cancer. Nat Med 24:724-730 (2018). Read more (PubMed: 29867227) »
  • Parra ER  et al. Comparison of Different Antibody Clones for Immunohistochemistry Detection of Programmed Cell Death Ligand 1 (PD-L1) on Non-Small Cell Lung Carcinoma. Appl Immunohistochem Mol Morphol 26:83-93 (2018). Read more (PubMed: 28719380) »
See all 21 Publications for this product

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