The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use 0.25µg for 105-8 cells.
A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL.
ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
Involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production.
Highly expressed in heart, placenta, pancreas, lung and liver and weakly expressed in spleen, lymph nodes and thymus.
Belongs to the immunoglobulin superfamily. BTN/MOG family. Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Flow cytometric analysis of mouse PD-L2-transfected L5178Y cells labeling PD-L2 with 0.125 µg ab171259 (filled histogram) with 0.25 µg Rat IgG2a Isotype Control (open histogram) followed by anti-Rat IgG biotin and streptavidin PE. Total viable cells were used for analysis.