Recombinant
RabMAb

Recombinant Anti-PD1 antibody [EPR20665] (ab214421)

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Overview

  • Product name

    Anti-PD1 antibody [EPR20665]
    See all PD1 primary antibodies

  • Description

    Rabbit monoclonal [EPR20665] to PD1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    This product was produced with the following immunogens:
    Synthetic peptide corresponding to Mouse PD1 aa 100-200 (Cysteine residue).
    Database link: Q02242

    Synthetic peptide corresponding to Mouse PD1 aa 50-150 (Cysteine residue).
    Database link: Q02242

  • Positive control

    • WB: EL4.IL-2 (mouse lymphoma T lymphocyte), whole cell lysate. IHC-P: Mouse spleen and thymus tissues.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab214421 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 50-55 kDa (predicted molecular weight: 31 kDa).
IHC-P 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/100.

Target

  • Function

    Possible cell death inducer, in association with other factors.

  • Involvement in disease

    Genetic variation in PDCD1 is associated with susceptibility to systemic lupus erythematosus type 2 (SLEB2) [MIM:605218]. Systemic lupus erythematosus is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.

  • Sequence similarities

    Contains 1 Ig-like V-type (immunoglobulin-like) domain.

  • Developmental stage

    Induced at programmed cell death.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • CD279 antibody
      • CD279 antigen antibody
      • hPD 1 antibody
      • hPD l antibody
      • hPD-1 antibody
      • hSLE1 antibody
      • PD 1 antibody
      • PD-1 antibody
      • PD1 antibody
      • PDCD 1 antibody
      • PDCD1 antibody
      • PDCD1_HUMAN antibody
      • Programmed cell death 1 antibody
      • Programmed cell death 1 protein antibody
      • Programmed cell death protein 1 antibody
      • Protein PD 1 antibody
      • Protein PD-1 antibody
      • SLEB2 antibody
      • Systemic lupus erythematosus susceptibility 2 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [EPR20665] (ab214421)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [EPR20665] (ab214421)

      Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling PD1 with ab214421 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on the germinal center of mouse spleen. (PMID: 21750671, PMID: 17640856). Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-PD1 antibody [EPR20665] (ab214421)
      Immunocytochemistry/ Immunofluorescence - Anti-PD1 antibody [EPR20665] (ab214421)

      Immunofluorescent analysis of 100% methanol-fixed EL4.IL-2 (mouse lymphoma T lymphocyte) cells labeling PD1 with ab214421 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on EL4.IL-2 cell line.

      The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    • Western blot - Anti-PD1 antibody [EPR20665] (ab214421)
      Western blot - Anti-PD1 antibody [EPR20665] (ab214421)
      Anti-PD1 antibody [EPR20665] (ab214421) at 1/1000 dilution + EL4.IL-2 (mouse lymphoma T lymphocyte) whole cell lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Developed using the ECL technique.

      Predicted band size: 31 kDa
      Observed band size: 50-55 kDa
      why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [EPR20665] (ab214421)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [EPR20665] (ab214421)

      Immunohistochemical analysis of paraffin-embedded mouse thymus tissue labeling PD1 with ab214421 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Sporadic cytoplasmic staining on mouse thymus medulla. (PMID: 21750671, PMID: 17640856). Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    References

    Publishing research using ab214421? Please let us know so that we can cite the reference in this datasheet.

    ab214421 has been referenced in 8 publications.

    • Tang L  et al. PD-L1 Ameliorates Murine Acute Graft-Versus-Host Disease by Suppressing Effector But Not Regulatory T Cells Function. Arch Immunol Ther Exp (Warsz) 67:179-187 (2019). PubMed: 30927016
    • Pénzváltó Z  et al. A Syngeneic ErbB2 Mammary Cancer Model for Preclinical Immunotherapy Trials. J Mammary Gland Biol Neoplasia N/A:N/A (2019). PubMed: 30810966
    • Lin X  et al. Combined a-programmed death-1 monoclonal antibody blockade and fractionated radiation therapy reduces tumor growth in mouse EL4 lymphoma. Cancer Biol Ther 20:666-679 (2019). PubMed: 30572778
    • Zhou WY  et al. Long noncoding RNA LINC00473 drives the progression of pancreatic cancer via upregulating programmed death-ligand 1 by sponging microRNA-195-5p. J Cell Physiol 234:23176-23189 (2019). PubMed: 31206665
    • Tian S  et al. Treatment of Type II Collagen-Induced Rat Rheumatoid Arthritis Model by Interleukin 10 (IL10)-Mesenchymal Stem Cells (BMSCs). Med Sci Monit 25:2923-2934 (2019). PubMed: 31005957
    • Lu J  et al. Breast Cancer Chemo-immunotherapy through Liposomal Delivery of an Immunogenic Cell Death Stimulus Plus Interference in the IDO-1 Pathway. ACS Nano 12:11041-11061 (2018). PubMed: 30481959
    • Wang J  et al. Activation of PGE2/EP2 and PGE2/EP4 signaling pathways positively regulate the level of PD-1 in infiltrating CD8+T cells in patients with lung cancer. Oncol Lett 15:552-558 (2018). PubMed: 29285200
    • Guo LL  et al. Recombinant adenovirus expressing a dendritic cell-targeted melanoma surface antigen for tumor-specific immunotherapy in melanoma mice model. Exp Ther Med 15:5394-5402 (2018). PubMed: 29844804

    Customer reviews and Q&As

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    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PD1 antibody [EPR20665]

     Inconclusive
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Lewis Lung Carcinoma)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate Buffer, Vector laboratories(H-3300)
    Permeabilization
    Yes - 0.2% Triton-X
    Specification
    Lewis Lung Carcinoma
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Anugraha Rajagopalan

    Verified customer

    Submitted Mar 16 2018

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