RecombinantRabMAb

Recombinant Anti-PD1 antibody [EPR20665] (ab214421)

Reviews (2) Submit a question References (15)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [EPR20665] (ab214421)
  • Immunocytochemistry/ Immunofluorescence - Anti-PD1 antibody [EPR20665] (ab214421)
  • Western blot - Anti-PD1 antibody [EPR20665] (ab214421)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [EPR20665] (ab214421)
  • Anti-PD1 antibody [EPR20665] (ab214421)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20665] to PD1
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-PD1 antibody [EPR20665]
    See all PD1 primary antibodies

  • Description

    Rabbit monoclonal [EPR20665] to PD1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: IHC-Fr

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    This product was produced with the following immunogens:
    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: EL4.IL-2 (mouse lymphoma T lymphocyte), whole cell lysate. IHC-P: Mouse spleen and thymus tissues.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab214421 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Detects a band of approximately 50-55 kDa (predicted molecular weight: 31 kDa).
IHC-P (2)
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/100.
Notes
WB
1/1000. Detects a band of approximately 50-55 kDa (predicted molecular weight: 31 kDa).
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/100.
  • Application notes
    Is unsuitable for IHC-Fr.

    • Target

    • Function

      Possible cell death inducer, in association with other factors.

    • Involvement in disease

      Genetic variation in PDCD1 is associated with susceptibility to systemic lupus erythematosus type 2 (SLEB2) [MIM:605218]. Systemic lupus erythematosus is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.

    • Sequence similarities

      Contains 1 Ig-like V-type (immunoglobulin-like) domain.

    • Developmental stage

      Induced at programmed cell death.

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • CD279 antibody
        • CD279 antigen antibody
        • hPD 1 antibody
        • hPD l antibody
        • hPD-1 antibody
        • hSLE1 antibody
        • PD 1 antibody
        • PD-1 antibody
        • PD1 antibody
        • PDCD 1 antibody
        • PDCD1 antibody
        • PDCD1_HUMAN antibody
        • Programmed cell death 1 antibody
        • Programmed cell death 1 protein antibody
        • Programmed cell death protein 1 antibody
        • Protein PD 1 antibody
        • Protein PD-1 antibody
        • SLEB2 antibody
        • Systemic lupus erythematosus susceptibility 2 antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [EPR20665] (ab214421)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [EPR20665] (ab214421)

        Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling PD1 with ab214421 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on the germinal center of mouse spleen. (PMID: 21750671, PMID: 17640856). Counter stained with hematoxylin.

        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

        Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

      • Immunocytochemistry/ Immunofluorescence - Anti-PD1 antibody [EPR20665] (ab214421)
        Immunocytochemistry/ Immunofluorescence - Anti-PD1 antibody [EPR20665] (ab214421)

        Immunofluorescent analysis of 100% methanol-fixed EL4.IL-2 (mouse lymphoma T lymphocyte) cells labeling PD1 with ab214421 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on EL4.IL-2 cell line.

        The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

      • Western blot - Anti-PD1 antibody [EPR20665] (ab214421)
        Western blot - Anti-PD1 antibody [EPR20665] (ab214421)
        Anti-PD1 antibody [EPR20665] (ab214421) at 1/1000 dilution + EL4.IL-2 (mouse lymphoma T lymphocyte) whole cell lysate at 10 µg

        Secondary
        Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

        Developed using the ECL technique.

        Predicted band size: 31 kDa
        Observed band size: 50-55 kDa why is the actual band size different from the predicted?


        Exposure time: 3 minutes


        Blocking/Dilution buffer: 5% NFDM/TBST.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [EPR20665] (ab214421)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [EPR20665] (ab214421)

        Immunohistochemical analysis of paraffin-embedded mouse thymus tissue labeling PD1 with ab214421 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Sporadic cytoplasmic staining on mouse thymus medulla. (PMID: 21750671, PMID: 17640856). Counter stained with hematoxylin.

        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

        Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

      • Anti-PD1 antibody [EPR20665] (ab214421)
        Anti-PD1 antibody [EPR20665] (ab214421)

      References (15)

      Publishing research using ab214421? Please let us know so that we can cite the reference in this datasheet.

      ab214421 has been referenced in 15 publications.

      • Liu Y  et al. Bcl-6-directed follicular helper T cells promote vascular inflammatory injury in diabetic retinopathy. Theranostics 10:4250-4264 (2020). PubMed: 32226551
      • Lu SW  et al. IL-20 antagonist suppresses PD-L1 expression and prolongs survival in pancreatic cancer models. Nat Commun 11:4611 (2020). PubMed: 32929072
      • Fu Y  et al. An HNSCC syngeneic mouse model for tumor immunology research and preclinical evaluation. Int J Mol Med 46:1501-1513 (2020). PubMed: 32700748
      • Muralidharan A  et al. PD-1 of Sigmodon hispidus: Gene identification, characterization and preliminary evaluation of expression in inactivated RSV vaccine-induced enhanced respiratory disease. Sci Rep 9:11638 (2019). PubMed: 31406266
      • Li Y  et al. The anthelmintic flubendazole blocks human melanoma growth and metastasis and suppresses programmed cell death protein-1 and myeloid-derived suppressor cell accumulation. Cancer Lett 459:268-276 (2019). PubMed: 31128215
      View all Publications for this product

      Customer reviews and Q&As

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      1-2 of 2 Abreviews or Q&A

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PD1 antibody [EPR20665]

       Excellent
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (Thymus)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH9
      Permeabilization
      No
      Specification
      Thymus
      Blocking step
      Serum as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
      Fixative
      10% NBF
      Read More

      Vicki Shone

      Verified customer

      Submitted Feb 03 2021

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PD1 antibody [EPR20665]

       Inconclusive
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (Lewis Lung Carcinoma)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Citrate Buffer, Vector laboratories(H-3300)
      Permeabilization
      Yes - 0.2% Triton-X
      Specification
      Lewis Lung Carcinoma
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
      Fixative
      Paraformaldehyde
      Read More

      Anugraha Rajagopalan

      Verified customer

      Submitted Mar 16 2018

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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