Anti-PD1 antibody [J116] (ab140950)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-PD1 antibody [J116]
See all PD1 primary antibodies -
Description
Mouse monoclonal [J116] to PD1 -
Host species
Mouse -
Tested applications
Suitable for: WB, IP, IHC-P, IHC-Fr, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Human PD1 protein
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Positive control
- Human tonsil tissue; Human PD1 transfected cells
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.88% Sodium chloride, 99% PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
J116 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab140950 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. Predicted molecular weight: 32 kDa. | |
| IP | Use at an assay dependent concentration. | |
| IHC-P | Use a concentration of 20 µg/ml. | |
| IHC-Fr | Use at an assay dependent concentration. | |
| Flow Cyt | Use 1µg for 105-8 cells. (In a final volume of 100 µl).
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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Target
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Function
Possible cell death inducer, in association with other factors. -
Involvement in disease
Genetic variation in PDCD1 is associated with susceptibility to systemic lupus erythematosus type 2 (SLEB2) [MIM:605218]. Systemic lupus erythematosus is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Developmental stage
Induced at programmed cell death. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 5133 Human
- Omim: 600244 Human
- SwissProt: Q15116 Human
- Unigene: 158297 Human
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Alternative names
- CD279 antibody
- CD279 antigen antibody
- hPD 1 antibody
see all
Images
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![Immunohistochemistry (Frozen sections) - Anti-PD1 antibody [J116] (ab140950)](https://www.abcam.com/ps/products/140/ab140950/Images/ab140950-255496-anti-pd1-antibody-j116-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-PD1 antibody [J116] (ab140950)This image is courtesy of an anonymous Abreview.ab140950 staining PD1 in human atopic dermatitis tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with methanol and blocked with 4% serum for 30 minutes at 25°C. Samples were incubated with primary antibody (20μg/ml) for 16 hours at 4°C. A Biotin-conjugated anti-mouse IgG polyclonal (10μg/ml) was used as the secondary antibody.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [J116] (ab140950)](https://www.abcam.com/ps/products/140/ab140950/Images/ab140950-156412-anti-pd1-antibody-j116-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [J116] (ab140950)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labelling PD1 with ab140950 at 20 µg/ml. -
![Flow Cytometry - Anti-PD1 antibody [J116] (ab140950)](https://www.abcam.com/ps/products/140/ab140950/Images/ab140950-275248-anti-pd1-antibody-j116-flow-cytometry.jpg)
Flow Cytometry - Anti-PD1 antibody [J116] (ab140950)This image is courtesy of an anonymous abreview.Flow Cytometry analysis of peripheral blood cells, separated by Ficoll density separation labeling PD1 with ab140950 at 1µg/ml. PBS with human IgG (Fc-block) was used as the sample buffer. A goat anti-mouse FITC conjugated secondary antibody was used at 1/100 dilution. Isotype control (1), Untreated control (2), PHA treated (5ng/ml) (3).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [J116] (ab140950)](https://www.abcam.com/ps/products/140/ab140950/Images/ab140950-255487-anti-pd1-antibody-j116-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [J116] (ab140950)This image is courtesy of an anonymous Abreview.ab140950 staining PD1 in human nasopharynx tissue sections by Immunohistochemistry (Formalin/PFA-fixed frozen paraffin-embedded sections). Tissue samples were fixed with paraformaldehyde and blocked with 4% serum for 30 minutes at 25°C and antigen retrieval was by heat mediation in citrate buffer (pH 6.0). The sample was incubated with primary antibody (20ug/ml) in blocking buffer at 4°C for 16 hours. An Alexa Fluor® 488-conjugated Goat anti-mouse polyclonal (1/100) was used as the secondary antibody.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Yang MW et al. Clinical significance of programmed death 1/programmed death ligand 1 pathway in gastric neuroendocrine carcinomas. World J Gastroenterol 25:1684-1696 (2019). Read more (PubMed: 31011254) »
