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    pd1-antibody-j116-ab140950.pdf

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Cell Biology Apoptosis Receptors Death Receptors
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Anti-PD1 antibody [J116] (ab140950)

  • Datasheet
Reviews (4) Submit a question References (3)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [J116] (ab140950)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [J116] (ab140950)

Key features and details

  • Mouse monoclonal [J116] to PD1
  • Suitable for: IHC-P
  • Reacts with: Human
  • Isotype: IgG1

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Primary
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Alexa Fluor® 488 Anti-PD-L1 antibody [28-8] (ab209959)

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Overview

  • Product name

    Anti-PD1 antibody [J116]
    See all PD1 primary antibodies
  • Description

    Mouse monoclonal [J116] to PD1
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    See all applications and species data
  • Immunogen

    Human PD1 protein

  • Positive control

    • Human tonsil tissue; Human PD1 transfected cells

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.09% Sodium azide
    Constituents: 0.88% Sodium chloride, 99% PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    J116
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Cell Biology
    • Apoptosis
    • Receptors
    • Death Receptors
    • Immunology
    • Adaptive Immunity
    • T Cells
    • CD
    • Cancer
    • Tumor immunology
    • CD markers
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Other
    • Immunology
    • Adaptive Immunity
    • Regulatory T Cells
    • Cancer
    • Cell Death
    • Apoptosis
    • Other

Associated products

  • Alternative Versions

    • Anti-PD1 antibody [J116] - Low endotoxin, Azide free (ab171267)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant human PD1 protein (Active) (ab174035)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab140950 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
Application Abreviews Notes
IHC-P (1)
Use a concentration of 20 µg/ml.
Notes
IHC-P
Use a concentration of 20 µg/ml.

Target

  • Function

    Possible cell death inducer, in association with other factors.
  • Involvement in disease

    Genetic variation in PDCD1 is associated with susceptibility to systemic lupus erythematosus type 2 (SLEB2) [MIM:605218]. Systemic lupus erythematosus is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
  • Sequence similarities

    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Developmental stage

    Induced at programmed cell death.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession Q15116 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5133 Human
    • Omim: 600244 Human
    • SwissProt: Q15116 Human
    • Unigene: 158297 Human
    • Alternative names

      • CD279 antibody
      • CD279 antigen antibody
      • hPD 1 antibody
      • hPD l antibody
      • hPD-1 antibody
      • hSLE1 antibody
      • PD 1 antibody
      • PD-1 antibody
      • PD1 antibody
      • PDCD 1 antibody
      • PDCD1 antibody
      • PDCD1_HUMAN antibody
      • Programmed cell death 1 antibody
      • Programmed cell death 1 protein antibody
      • Programmed cell death protein 1 antibody
      • Protein PD 1 antibody
      • Protein PD-1 antibody
      • SLEB2 antibody
      • Systemic lupus erythematosus susceptibility 2 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [J116] (ab140950)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [J116] (ab140950)
      Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labelling PD1 with ab140950 at 20 µg/ml.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [J116] (ab140950)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [J116] (ab140950)This image is courtesy of an anonymous Abreview.

      ab140950 staining PD1 in human nasopharynx tissue sections by Immunohistochemistry (Formalin/PFA-fixed frozen paraffin-embedded sections). Tissue samples were fixed with paraformaldehyde and blocked with 4% serum for 30 minutes at 25°C and antigen retrieval was by heat mediation in citrate buffer (pH 6.0). The sample was incubated with primary antibody (20ug/ml) in blocking buffer at 4°C for 16 hours. An Alexa Fluor® 488-conjugated Goat anti-mouse polyclonal (1/100) was used as the secondary antibody.

      See Abreview

    Protocols

    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (3)

    Publishing research using ab140950? Please let us know so that we can cite the reference in this datasheet.

    ab140950 has been referenced in 3 publications.

    • Hu JQ  et al. IL-2 enhanced MHC class I expression in papillary thyroid cancer with Hashimoto's thyroiditis overcomes immune escape in vitro. J Cancer 11:4250-4260 (2020). PubMed: 32368308
    • Yang MW  et al. Clinical significance of programmed death 1/programmed death ligand 1 pathway in gastric neuroendocrine carcinomas. World J Gastroenterol 25:1684-1696 (2019). PubMed: 31011254
    • Wang B  et al. Effects of Combination of Anti-CTLA-4 and Anti-PD-1 on Gastric Cancer Cells Proliferation, Apoptosis and Metastasis. Cell Physiol Biochem 49:260-270 (2018). PubMed: 30138920

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-4 of 4 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-PD1 antibody [J116]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (skin (atopic dermatitis))
    Permeabilization
    No
    Specification
    skin (atopic dermatitis)
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
    Fixative
    Methanol
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 24 2015

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PD1 antibody [J116]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (nasopharynx)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6,0
    Permeabilization
    No
    Specification
    nasopharynx
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 24 2015

    Flow Cytometry abreview for Anti-PD1 antibody [J116]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Human Cell (PBMCs)
    Permeabilization
    No
    Gating Strategy
    lympho and mono gate
    Specification
    PBMCs
    Preparation
    Cell harvesting/tissue preparation method: peripheral blood cells, separated by Ficoll density separation
    Sample buffer: PBS with human IgG (Fc-block)
    Fixation
    no fixation
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 25 2015

    Western blot abreview for Anti-PD1 antibody [J116]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (PBMCs)
    Gel Running Conditions
    Reduced Non-Denaturing (Native) (5-16% gels)
    Loading amount
    15 µg
    Treatment
    see below
    Specification
    PBMCs
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 25 2015

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