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Human PD1 protein
Our Abpromise guarantee covers the use of ab140950 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 32 kDa.|
|IP||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 20 µg/ml.|
|IHC-Fr||Use at an assay dependent concentration.|
|Flow Cyt||Use 1µg for 105-8 cells.
(In a final volume of 100 µl).
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ab140950 staining PD1 in human atopic dermatitis tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with methanol and blocked with 4% serum for 30 minutes at 25°C. Samples were incubated with primary antibody (20μg/ml) for 16 hours at 4°C. A Biotin-conjugated anti-mouse IgG polyclonal (10μg/ml) was used as the secondary antibody.
Flow Cytometry analysis of peripheral blood cells, separated by Ficoll density separation labeling PD1 with ab140950 at 1µg/ml. PBS with human IgG (Fc-block) was used as the sample buffer. A goat anti-mouse FITC conjugated secondary antibody was used at 1/100 dilution. Isotype control (1), Untreated control (2), PHA treated (5ng/ml) (3).
ab140950 staining PD1 in human nasopharynx tissue sections by Immunohistochemistry (Formalin/PFA-fixed frozen paraffin-embedded sections). Tissue samples were fixed with paraformaldehyde and blocked with 4% serum for 30 minutes at 25°C and antigen retrieval was by heat mediation in citrate buffer (pH 6.0). The sample was incubated with primary antibody (20ug/ml) in blocking buffer at 4°C for 16 hours. An Alexa Fluor® 488-conjugated Goat anti-mouse polyclonal (1/100) was used as the secondary antibody.
ab140950 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"