Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [NAT105] to PD1
- Suitable for: ICC/IF, WB, IHC-P, Flow Cyt
- Reacts with: Human
Product nameAnti-PD1 antibody [NAT105]
See all PD1 primary antibodies
DescriptionMouse monoclonal [NAT105] to PD1
Tested applicationsSuitable for: ICC/IF, WB, IHC-P, Flow Cytmore details
Species reactivityReacts with: Human
Tissue, cells or virus within Human PD1. The exact sequence is proprietary.
Database link: Q15116
- WB: MOLT-4 treated with 500 ng/ml ionomycin and 10 ng/ml phorbol-12-myristate-13-acetate (PMA) for 24 hours, whole cell lysate. IHC-P: Human tonsil tissue. Flow Cyt: MOLT-4 cells treated with ionomycin (500 ng/ml, 24h) and PMA (10 ng/ml, 24h). ICC/IF: MOLT-4 cells treated with Ionomycin (500ng/ml, 24 h) and PMA (10ng/ml, 24 h)
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol
Concentration information loading...
PurityProtein G purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab234444 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Use under non reducing condition. Predicted molecular weight: 32 kDa.|
Perform heat-mediated antigen retrieval using Sodium citrate buffer (pH 6.0), 20 mins.
FunctionPossible cell death inducer, in association with other factors.
Involvement in diseaseGenetic variation in PDCD1 is associated with susceptibility to systemic lupus erythematosus type 2 (SLEB2) [MIM:605218]. Systemic lupus erythematosus is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
Developmental stageInduced at programmed cell death.
- Information by UniProt
- CD279 antibody
- CD279 antigen antibody
- hPD 1 antibody
Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling PD1 with ab234444 at 1/50 dilution, followed by Rabbit Anti-Mouse IgG + Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on T cells of human tonsil germinal center is observed. Performed on a Leica Biosystems BOND instrument. Counter stained with hematoxylin.
Ab234444 staining PD1 in the MOLT-4 (human lymphoblastic leukemia T lymphoblast) treated with Ionomycin (500 ng/ml, 24 h) and PMA (10 ng/ml, 24 h) by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde, permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody (1/50). ab150117 anti-mouse IgG H&L (Alexa Fluor® 488) preadsorbed (1/100) was used as the secondary antibody.
DAPI was used as a Nuclear counter stain.
Flow cytometric analysis of MOLT-4 (human lymphoblastic leukemia cell line) cell line treated with ionomycin (500 ng/ml, 24h) and PMA (10 ng/ml, 24h) labeling PD1 with ab234444 at 1/1000 dilution (red) and an untreated control (green) compared with a Mouse monoclonal IgG1 (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.
All lanes : Anti-PD1 antibody [NAT105] (ab234444) at 1/1000 dilution
Lane 1 : Untreated MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate
Lane 2 : MOLT-4 (human lymphoblastic leukemia cell line) treated with 500 ng/ml ionomycin and 10 ng/ml phorbol-12-myristate-13-acetate (PMA) for 24 hours, whole cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-Mouse IgG (H+L) Peroxidase Peroxidase-Conjugated at 1/5000 dilution
Predicted band size: 32 kDa
Exposure time: 10 seconds
Blocking/Dilution buffer: 5% NFDM/TBST.
The expression profile observed is consistent with what has been described in the literature (PMID: 26318293)
We recommend that the customer optimizes the blotting conditions for themselves.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab234444 has not yet been referenced specifically in any publications.