Anti-PD1 antibody [NAT105] (ab234444)
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Overview
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Product nameAnti-PD1 antibody [NAT105]
See all PD1 primary antibodies -
DescriptionMouse monoclonal [NAT105] to PD1
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Host speciesMouse
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Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
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Species reactivityReacts with: Human
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Immunogen
Tissue, cells or virus within Human PD1. The exact sequence is proprietary.
Database link: Q15116 -
Positive control
- WB: MOLT-4 treated with 500 ng/ml ionomycin and 10 ng/ml phorbol-12-myristate-13-acetate (PMA) for 24 hours, whole cell lysate. IHC-P: Human tonsil tissue. Flow Cyt: MOLT-4 cells treated with ionomycin (500 ng/ml, 24h) and PMA (10 ng/ml, 24h).
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol -
Concentration information loading... -
PurityProtein G purified
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ClonalityMonoclonal
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Clone numberNAT105
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IsotypeIgG1
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Light chain typekappa
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Related Products
Applications
Our Abpromise guarantee covers the use of ab234444 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Use under non reducing condition. Predicted molecular weight: 32 kDa. | |
| IHC-P | 1/50. Perform heat-mediated antigen retrieval using Sodium citrate buffer (pH 6.0), 20 mins. |
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| Flow Cyt | 1/100. |
Target
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FunctionPossible cell death inducer, in association with other factors.
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Involvement in diseaseGenetic variation in PDCD1 is associated with susceptibility to systemic lupus erythematosus type 2 (SLEB2) [MIM:605218]. Systemic lupus erythematosus is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
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Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
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Developmental stageInduced at programmed cell death.
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Cellular localizationMembrane.
- Information by UniProt
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Database links
- Entrez Gene: 5133 Human
- Omim: 600244 Human
- SwissProt: Q15116 Human
- Unigene: 158297 Human
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Alternative names
- CD279 antibody
- CD279 antigen antibody
- hPD 1 antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [NAT105] (ab234444)](https://www.abcam.com/ps/products/234/ab234444/Images/ab234444-313142-4444.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [NAT105] (ab234444)Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling PD1 with ab234444 at 1/50 dilution, followed by Rabbit Anti-Mouse IgG + Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on T cells of human tonsil germinal center is observed. Performed on a Leica Biosystems BOND instrument. Counter stained with hematoxylin.
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![Flow Cytometry - Anti-PD1 antibody [NAT105] (ab234444)](https://www.abcam.com/ps/products/234/ab234444/Images/ab234444-2-234444FC1.jpg)
Flow Cytometry - Anti-PD1 antibody [NAT105] (ab234444)Flow cytometric analysis of MOLT-4 (human lymphoblastic leukemia cell line) cell line treated with ionomycin (500 ng/ml, 24h) and PMA (10 ng/ml, 24h) labeling PD1 with ab234444 at 1/1000 dilution (red) and an untreated control (green) compared with a Mouse monoclonal IgG1 (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.
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![Western blot - Anti-PD1 antibody [NAT105] (ab234444)](https://www.abcam.com/ps/products/234/ab234444/Images/ab234444-1-234444WB1.jpg)
Western blot - Anti-PD1 antibody [NAT105] (ab234444)All lanes : Anti-PD1 antibody [NAT105] (ab234444) at 1/1000 dilution
Lane 1 : Untreated MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate
Lane 2 : MOLT-4 (human lymphoblastic leukemia cell line) treated with 500 ng/ml ionomycin and 10 ng/ml phorbol-12-myristate-13-acetate (PMA) for 24 hours, whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Mouse IgG (H+L) Peroxidase Peroxidase-Conjugated at 1/5000 dilution
Predicted band size: 32 kDa
Exposure time: 10 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
The expression profile observed is consistent with what has been described in the literature (PMID: 26318293)
We recommend that the customer optimizes the blotting conditions for themselves.
Datasheets and documents
References
ab234444 has not yet been referenced specifically in any publications.
