Overview

  • Product name
    Anti-PD1 antibody [NAT105]
    See all PD1 primary antibodies
  • Description
    Mouse monoclonal [NAT105] to PD1
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Tissue, cells or virus within Human PD1. The exact sequence is proprietary.
    Database link: Q15116

  • Positive control
    • WB: MOLT-4 treated with 500 ng/ml ionomycin and 10 ng/ml phorbol-12-myristate-13-acetate (PMA) for 24 hours, whole cell lysate. IHC-P: Human tonsil tissue. Flow Cyt: MOLT-4 cells treated with ionomycin (500 ng/ml, 24h) and PMA (10 ng/ml, 24h).

Properties

Applications

Our Abpromise guarantee covers the use of ab234444 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Use under non reducing condition. Predicted molecular weight: 32 kDa.

See Western blot protocol.

IHC-P 1/50.

Perform heat-mediated antigen retrieval using Sodium citrate buffer (pH 6.0), 20 mins.

Flow Cyt 1/100.

Target

  • Function
    Possible cell death inducer, in association with other factors.
  • Involvement in disease
    Genetic variation in PDCD1 is associated with susceptibility to systemic lupus erythematosus type 2 (SLEB2) [MIM:605218]. Systemic lupus erythematosus is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
  • Sequence similarities
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Developmental stage
    Induced at programmed cell death.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD279 antibody
    • CD279 antigen antibody
    • hPD 1 antibody
    • hPD l antibody
    • hPD-1 antibody
    • hSLE1 antibody
    • PD 1 antibody
    • PD-1 antibody
    • PD1 antibody
    • PDCD 1 antibody
    • PDCD1 antibody
    • PDCD1_HUMAN antibody
    • Programmed cell death 1 antibody
    • Programmed cell death 1 protein antibody
    • Programmed cell death protein 1 antibody
    • Protein PD 1 antibody
    • Protein PD-1 antibody
    • SLEB2 antibody
    • Systemic lupus erythematosus susceptibility 2 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling PD1 with ab234444 at 1/50 dilution, followed by Rabbit Anti-Mouse IgG + Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on T cells of human tonsil germinal center is observed. Performed on a Leica Biosystems BOND instrument. Counter stained with hematoxylin.

  • Flow cytometric analysis of MOLT-4 (human lymphoblastic leukemia cell line) cell line treated with ionomycin (500 ng/ml, 24h) and PMA (10 ng/ml, 24h) labeling PD1 with ab234444 at 1/1000 dilution (red) and an untreated control (green) compared with a Mouse monoclonal IgG1 (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

  • All lanes : Anti-PD1 antibody [NAT105] (ab234444) at 1/1000 dilution

    Lane 1 : Untreated MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate
    Lane 2 : MOLT-4 (human lymphoblastic leukemia cell line) treated with 500 ng/ml ionomycin and 10 ng/ml phorbol-12-myristate-13-acetate (PMA) for 24 hours, whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-Mouse IgG (H+L) Peroxidase Peroxidase-Conjugated at 1/5000 dilution

    Predicted band size: 32 kDa


    Exposure time: 10 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The expression profile observed is consistent with what has been described in the literature (PMID: 26318293)

    We recommend that the customer optimizes the blotting conditions for themselves.

References

ab234444 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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