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Anti-PD1 antibody [NAT105] (ab52587)

  • Datasheet
  • SDS
Reviews (8)Q&A (3)References (152)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [NAT105] (ab52587)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [NAT105] (ab52587)
  • Flow Cytometry - Anti-PD1 antibody [NAT105] (ab52587)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [NAT105] (ab52587)
  • Western blot - Anti-PD1 antibody [NAT105] (ab52587)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [NAT105] (ab52587)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [NAT105] (ab52587)
  • Flow Cytometry - Anti-PD1 antibody [NAT105] (ab52587)
  • Immunocytochemistry/ Immunofluorescence - Anti-PD1 antibody [NAT105] (ab52587)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [NAT105] (ab52587)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [NAT105] (ab52587)
  • Immunohistochemistry (Frozen sections) - Anti-PD1 antibody [NAT105] (ab52587)
  • Immunohistochemistry (Frozen sections) - Anti-PD1 antibody [NAT105] (ab52587)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [NAT105] (ab52587)

Key features and details

  • Mouse monoclonal [NAT105] to PD1
  • Suitable for: WB, Flow Cyt, IHC-Fr, ICC/IF, IHC-P
  • Reacts with: Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-PD1 antibody [NAT105]
    See all PD1 primary antibodies
  • Description

    Mouse monoclonal [NAT105] to PD1
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, Flow Cyt, IHC-Fr, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Tissue, cells or virus corresponding to Human PD1. Specifically, human YT cells (NK-like leukemia cell line) that express PD1.
    Database link: Q15116

  • Positive control

    • IHC-P: Human tonsil tissue. ICC/IF: MOLT-4 cells. Flow Cyt: Human tonsil extract. MOLT-4 cells. IHC-Fr: Human tonsil tissue.
  • General notes

    Please note that PD-1 is expressed variably in different tissues and that optimisation may be required depending on the tissue used for the experiment.

    Western blot protocol advice:

    Due to low expression of PD-1, we recommend loading a high amount of sample (100 µg) to detect the band for PD-1. Human tonsil and YT cell line lysates are suitable positive controls.

    New alternative versions of the NAT105 clone available:
    Recombinant version (ab234444)
    PBS-only recombinant version (ab201811)
    Chimeric recombinant rabbit version (ab216352)

    To test multiple PD-1 monoclonal antibodies please see our PD-1 trial-size panel – ab252192

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 6.97% L-Arginine
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    NAT105
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Cell Biology
    • Apoptosis
    • Receptors
    • Death Receptors
    • Immunology
    • Adaptive Immunity
    • T Cells
    • CD
    • Cancer
    • Tumor immunology
    • CD markers
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Other
    • Immunology
    • Adaptive Immunity
    • Regulatory T Cells
    • Cancer
    • Cell Death
    • Apoptosis
    • Other

Associated products

  • Alternative Versions

    • Anti-PD1 antibody [NAT105] - BSA and Azide free (ab201811)
    • Anti-PD1 antibody [NAT105] - Chimeric (ab216352)
    • Alexa Fluor® 488 Anti-PD1 antibody [NAT105] (ab220300)
    • Alexa Fluor® 647 Anti-PD1 antibody [NAT105] (ab220301)
    • PE Anti-PD1 antibody [NAT105] (ab220302)
    • Anti-PD1 antibody [NAT105] (ab234444)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150117)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant human PD1 protein (Active) (ab174035)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)

Applications

Our Abpromise guarantee covers the use of ab52587 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50. Predicted molecular weight: 32 kDa.
Flow Cyt 1/100.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-Fr Use at an assay dependent concentration.
ICC/IF Use a concentration of 5 - 10 µg/ml.

We recommend Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150117) secondary antibody.

IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function

    Possible cell death inducer, in association with other factors.
  • Involvement in disease

    Genetic variation in PDCD1 is associated with susceptibility to systemic lupus erythematosus type 2 (SLEB2) [MIM:605218]. Systemic lupus erythematosus is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
  • Sequence similarities

    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Developmental stage

    Induced at programmed cell death.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession Q15116 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5133 Human
    • Omim: 600244 Human
    • SwissProt: Q15116 Human
    • Unigene: 158297 Human
    • Alternative names

      • CD279 antibody
      • CD279 antigen antibody
      • hPD 1 antibody
      • hPD l antibody
      • hPD-1 antibody
      • hSLE1 antibody
      • PD 1 antibody
      • PD-1 antibody
      • PD1 antibody
      • PDCD 1 antibody
      • PDCD1 antibody
      • PDCD1_HUMAN antibody
      • Programmed cell death 1 antibody
      • Programmed cell death 1 protein antibody
      • Programmed cell death protein 1 antibody
      • Protein PD 1 antibody
      • Protein PD-1 antibody
      • SLEB2 antibody
      • Systemic lupus erythematosus susceptibility 2 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [NAT105] (ab52587)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [NAT105] (ab52587)

      IHC image of PD1 staining in normal human tonsil formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab52587 at 5 µg/ml for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [NAT105] (ab52587)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [NAT105] (ab52587)
      Double immunofluorescence staining of CD3 (green) and PD1 (red) on paraffin embedded tonsil.
    • Flow Cytometry - Anti-PD1 antibody [NAT105] (ab52587)
      Flow Cytometry - Anti-PD1 antibody [NAT105] (ab52587)

      Sample: Human tonsil cell extract.
      Dilution: ab52587 antibody was used as 1/200 in 1x106 cells/tube.
      Anti-CD4 antibody was used as 1/200 dilution.
      Anti-CD8 antibody was used as 1/200 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [NAT105] (ab52587)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [NAT105] (ab52587)Image courtesy of an AbReview submitted by Dr Hajnalka Rajnai

      Formaldehyde-fixed, paraffin-embedded human follicular lymphoma tissue stained for PD1 with ab52587 at 1/100 dilution in immunohistochemical analysis.

      See Abreview

    • Western blot - Anti-PD1 antibody [NAT105] (ab52587)
      Western blot - Anti-PD1 antibody [NAT105] (ab52587)This image is courtesy of an anonymous collaborator.
      Anti-PD1 antibody [NAT105] (ab52587) at 1/50 dilution + YT cell line extracts

      Predicted band size: 32 kDa
      Observed band size: 47 kDa
      why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [NAT105] (ab52587)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [NAT105] (ab52587)Schmidt et al PLoS One. 2015 Aug 27;10(8):e0136023. doi: 10.1371/journal.pone.0136023. eCollection 2015. Fig 1. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

      Representative immunohistochemical staining results for PD1 using ab52587 at a 1/50 dilution in human formalin-fixed, paraffin-embedded tissue specimens.

      Panel A: Normal lung tissue, negative control;

      Panel B: Tonsillar tissue, positive control;

      Panel C: PD1-negative tumor infiltrating lymphocytes;

      Panel D: PD1-positive tumor infiltrating lymphocytes in squamous cell carcinomas).

      Image from PMID: 26313362

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [NAT105] (ab52587)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [NAT105] (ab52587)Kim et al PLoS One. 2013 Dec 11;8(12):e82870. doi: 10.1371/journal.pone.0082870. eCollection 2013. Fig 1. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

      Immunohistochemical analysis of various soft tissue sarcomas staining PD1 using ab52587 at a 1/50 dilution.

      Arrows indicate PD1 positive lymphocytes.

      Image from PMID: 24349382

    • Flow Cytometry - Anti-PD1 antibody [NAT105] (ab52587)
      Flow Cytometry - Anti-PD1 antibody [NAT105] (ab52587)

      Overlay histogram showing MOLT-4 (Human lymphoblastic leukemia cell line) cells stained with ab52587 (red line). Live cells were incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab52587, 1/100 dilution) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150117) at 1/2000 dilution for 30 min at 4°C.

      A mouse IgG1 isotype control antibody  (ab170190) was used at the same concentration and conditions as the primary antibody (black line). Unlabeled sample (blue line) was also used as a control.
       
      Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.
    • Immunocytochemistry/ Immunofluorescence - Anti-PD1 antibody [NAT105] (ab52587)
      Immunocytochemistry/ Immunofluorescence - Anti-PD1 antibody [NAT105] (ab52587)

      MOLT-4 (Human lymphoblastic leukemia cell line) cells stained for PD1 (colored green) using ab52587 in ICC/IF.

      Cells were fixed with 100% methanol (5 min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% Tween-20 for 1 hour at room temperature to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with ab52587 at 10 µg/ml and ab6046 (Rabbit polyclonal to beta Tubulin - Loading Control) at 1 µg/ml overnight at +4°C. The secondary antibodies used were Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150117) secondary antibody used at 1 µg/ml (colored green) and Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150080) secondary antibody (pseudo-colored red) used at 2 µg/ml for 1 hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43 µM for 1 hour at room temperature.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [NAT105] (ab52587)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [NAT105] (ab52587)This image is from PubMedId: 27777963. Kaewkangsadan V et al. (2016) Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

      Immunohistochemical analysis of human large and locally advanced breast cancers staining PD1 using ab52587.

      (Panel a) Low level of PD-1+ T cell infiltration.

      (Panel b) high level of PD-1+ T cell infiltration. (Itu: intratumoral Str: stromal).

      Image data from PMID: 27777963

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [NAT105] (ab52587)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [NAT105] (ab52587)
      Double immunoenzymatic staining of Ki67 (brown) and PD1 (red) on paraffin embedded tonsil.
    • Immunohistochemistry (Frozen sections) - Anti-PD1 antibody [NAT105] (ab52587)
      Immunohistochemistry (Frozen sections) - Anti-PD1 antibody [NAT105] (ab52587)

      Human tonsil tissue stained for PD1 with ab52587 incubated for 30 mins at a 1/100 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Frozen sections) - Anti-PD1 antibody [NAT105] (ab52587)
      Immunohistochemistry (Frozen sections) - Anti-PD1 antibody [NAT105] (ab52587)This image is courtesy of an anonymous Abreview

      Immunohistochemical analysis of frozen human liver tissue labeling PD1 with ab52587 at 1/50 dilution.

      See Abreview

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [NAT105] (ab52587)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [NAT105] (ab52587)Argüello et al PLoS Negl Trop Dis. 2014 Aug 21;8(8):e2989. doi: 10.1371/journal.pntd.0002989. eCollection 2014 Aug. Fig 2. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

      Expression of markers of T cell differentiation and degree of inflammation in the heart of chronically T. cruzi-infected subjects with severe cardiomyopathy.

      A–D, left panel: representative photos of CD45RO, CD27, PD1 and CD57 expression, respectively.

      Panel C shown.

      Image data from PMID: 25144227

    Protocols

    • Flow cytometry protocols
    • Immunoprecipitation protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (152)

    Publishing research using ab52587? Please let us know so that we can cite the reference in this datasheet.

    ab52587 has been referenced in 152 publications.

    • Ando M  et al. Long-term eradication of extranodal natural killer/T-cell lymphoma, nasal type, by induced pluripotent stem cell-derived Epstein-Barr virus-specific rejuvenated T cells in vivo. Haematologica 105:796-807 (2020). PubMed: 31296577
    • Qian J  et al. High expression levels of TLR9 and PD-L1 indicates a poor prognosis in patients with angioimmunoblastic T-cell lymphoma: a retrospective study of 88 cases in a single center. J Cancer 11:57-68 (2020). PubMed: 31892973
    • Ito S  et al. Prognostic Significance of PD-1, PD-L1 and CD8 Gene Expression Levels in Gastric Cancer. Oncology N/A:1-11 (2020). PubMed: 32380498
    • Liu Y  et al. PD-1-Mediated PI3K/Akt/mTOR, Caspase 9/Caspase 3 and ERK Pathways Are Involved in Regulating the Apoptosis and Proliferation of CD4+ and CD8+ T Cells During BVDV Infection in vitro. Front Immunol 11:467 (2020). PubMed: 32256500
    • Akiyama M  et al. PD-L1 expression in malignant melanomas of the skin and gastrointestinal tract. Oncol Lett 19:2481-2488 (2020). PubMed: 32194748
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 11 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PD1 [NAT105] antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Leica ER2
    Sample
    Monkey Tissue sections (Lymphoid tisssues - spleen, tonsil and lymph node)
    Specification
    Lymphoid tisssues - spleen, tonsil and lymph node
    Permeabilization
    No
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 13 2014

    Immunohistochemistry (Frozen sections) abreview for Anti-PD1 antibody [NAT]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Liver)
    Specification
    Liver
    Fixative
    Acetone/Methanol 1:2
    Permeabilization
    Yes - TBS + 0,1% TritonX100
    Blocking step
    Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 15 2010

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PD1 antibody [NAT]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Follicular Lymphoma, Lymph node)
    Specification
    Follicular Lymphoma, Lymph node
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate Buffer, pH6
    Blocking step
    Novolink Protein Block, Novocastra, Leica Microsystems Inc. as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: 25°C
    Read More

    Dr. Hajnalka Rajnai

    Verified customer

    Submitted Nov 30 2009

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-PD1 antibody [NAT105]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Sheep Tissue sections (lung)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate buffer
    Permeabilization
    No
    Specification
    lung
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 25% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Ms. Anna Karagianni

    Verified customer

    Submitted Aug 11 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PD1 antibody [NAT105]

    Good
    Abreviews
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Human lymph node)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Low pH buffer
    Permeabilization
    No
    Specification
    Human lymph node
    Blocking step
    Dako endogenouse peroxidase block as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 18 2015

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PD1 antibody [NAT105]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Prostate Cancer)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate buffer pH 6.0
    Permeabilization
    No
    Specification
    Prostate Cancer
    Blocking step
    Serum as blocking agent for 15 minute(s) · Concentration: 10% · Temperature: 23°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 12 2015

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PD1 antibody [NAT105]

    Excellent
    Abreviews
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Dako Flex peroxidase as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: RT°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Low pH
    Sample
    Human Tissue sections (Malignant Melanoma)
    Specification
    Malignant Melanoma
    Permeabilization
    No
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 06 2015

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PD1 antibody [NAT105]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer ph 8,5
    Sample
    Human Tissue sections (tonsil)
    Specification
    tonsil
    Fixative
    Paraformaldehyde
    Read More

    Mr. Rudolf Jung

    Verified customer

    Submitted Oct 22 2014

    Question

    Since ab52587 is a mouse monoclonal I was wondering what secondary to use? We would like to titrate the Ab and am not sure how to go about doing it.

    Read More

    Abcam community

    Verified customer

    Asked on Jan 17 2013

    Answer

    .

    You may want to consider the following secondary antibodies:

    https://www.abcam.com/donkey-fab2-polyclonal-secondary-antibody-to-mouse-igg-hl-alexa-fluorreg-488-pre-adsorbed-ab150101.html

    https://www.abcam.com/donkey-fab2-polyclonal-secondary-antibody-to-mouse-igg-hl-alexa-fluor-647-pre-adsorbed-ab150103.html

    https://www.abcam.com/goat-polyclonal-secondary-antibody-to-mouse-igg1-gamma-chain-cy5--pre-adsorbed-ab136127.html

    https://www.abcam.com/goat-polyclonal-secondary-antibody-to-mouse-igg1-heavy-chain-fitc-ab97239.html

    https://www.abcam.com/goat-polyclonal-secondary-antibody-to-mouse-igg1-heavy-chain-allophycocyanin-pre-adsorbed-ab130786.html

    If you are concerned about the titration of the antibody, I would suggest trying the experiment with a range of different concentrations.

    Read More

    Abcam Scientific Support

    Answered on Jan 17 2013

    Question

    Can you tell me what the epitope of the antibody is directed against, or at least whether the antibody binds extracellular or cytoplasmic? We plan on running protein interaction studies, so we would need that information.

    Read More

    Abcam community

    Verified customer

    Asked on Jul 18 2012

    Answer

    I can confirm that the epitope of ab52587 is extracellular, because in FACS experiments fresh, unfixed cells were used.

    Read More

    Abcam Scientific Support

    Answered on Jul 18 2012

    1-10 of 11 Abreviews or Q&A

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