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    pd1-antibody-nat105-ab52587.pdf

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Cell Biology Apoptosis Receptors Death Receptors
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Anti-PD1 antibody [NAT105] (ab52587)

  • Datasheet
  • SDS
Reviews (9)Q&A (3)References (192)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [NAT105] (ab52587)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [NAT105] (ab52587)
  • Flow Cytometry - Anti-PD1 antibody [NAT105] (ab52587)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [NAT105] (ab52587)
  • Western blot - Anti-PD1 antibody [NAT105] (ab52587)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [NAT105] (ab52587)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [NAT105] (ab52587)
  • Flow Cytometry - Anti-PD1 antibody [NAT105] (ab52587)
  • Immunocytochemistry/ Immunofluorescence - Anti-PD1 antibody [NAT105] (ab52587)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [NAT105] (ab52587)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [NAT105] (ab52587)
  • Immunohistochemistry (Frozen sections) - Anti-PD1 antibody [NAT105] (ab52587)
  • Immunohistochemistry (Frozen sections) - Anti-PD1 antibody [NAT105] (ab52587)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [NAT105] (ab52587)

Key features and details

  • Mouse monoclonal [NAT105] to PD1
  • Suitable for: WB, Flow Cyt, IHC-Fr, ICC/IF, IHC-P
  • Reacts with: Human
  • Isotype: IgG1

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 PE

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Overview

  • Product name

    Anti-PD1 antibody [NAT105]
    See all PD1 primary antibodies
  • Description

    Mouse monoclonal [NAT105] to PD1
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, Flow Cyt, IHC-Fr, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human tonsil tissue. ICC/IF: MOLT-4 cells. Flow Cyt: Human tonsil extract. MOLT-4 cells. IHC-Fr: Human tonsil tissue.
  • General notes

    Please note that PD-1 is expressed variably in different tissues and that optimisation may be required depending on the tissue used for the experiment.

    Western blot protocol advice:

    Due to low expression of PD-1, we recommend loading a high amount of sample (100 µg) to detect the band for PD-1. Human tonsil and YT cell line lysates are suitable positive controls.

    New alternative versions of the NAT105 clone available:
    Recombinant version (ab234444)
    PBS-only recombinant version (ab201811)
    Chimeric recombinant rabbit version (ab216352)

    To test multiple PD-1 monoclonal antibodies please see our PD-1 trial-size panel – ab252192

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 6.97% L-Arginine
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    NAT105
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Cell Biology
    • Apoptosis
    • Receptors
    • Death Receptors
    • Immunology
    • Adaptive Immunity
    • T Cells
    • CD
    • Cancer
    • Tumor immunology
    • CD markers
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Other
    • Immunology
    • Adaptive Immunity
    • Regulatory T Cells
    • Cancer
    • Cell Death
    • Apoptosis
    • Other

Associated products

  • Alternative Versions

    • Anti-PD1 antibody [NAT105] - BSA and Azide free (ab201811)
    • Anti-PD1 antibody [NAT105] - Chimeric (ab216352)
    • Alexa Fluor® 488 Anti-PD1 antibody [NAT105] (ab220300)
    • Alexa Fluor® 647 Anti-PD1 antibody [NAT105] (ab220301)
    • PE Anti-PD1 antibody [NAT105] (ab220302)
    • Anti-PD1 antibody [NAT105] (ab234444)
    • Alexa Fluor® 555 Anti-PD1 antibody [NAT105] (ab280864)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150117)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant human PD1 protein (Active) (ab174035)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab52587 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/50. Predicted molecular weight: 32 kDa.
Flow Cyt
1/100.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-Fr (1)
Use at an assay dependent concentration.
ICC/IF
Use a concentration of 5 - 10 µg/ml.

We recommend Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150117) secondary antibody.

IHC-P (7)
1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Notes
WB
1/50. Predicted molecular weight: 32 kDa.
Flow Cyt
1/100.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-Fr
Use at an assay dependent concentration.
ICC/IF
Use a concentration of 5 - 10 µg/ml.

We recommend Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150117) secondary antibody.

IHC-P
1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function

    Possible cell death inducer, in association with other factors.
  • Involvement in disease

    Genetic variation in PDCD1 is associated with susceptibility to systemic lupus erythematosus type 2 (SLEB2) [MIM:605218]. Systemic lupus erythematosus is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
  • Sequence similarities

    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Developmental stage

    Induced at programmed cell death.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession Q15116 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5133 Human
    • Omim: 600244 Human
    • SwissProt: Q15116 Human
    • Unigene: 158297 Human
    • Alternative names

      • CD279 antibody
      • CD279 antigen antibody
      • hPD 1 antibody
      • hPD l antibody
      • hPD-1 antibody
      • hSLE1 antibody
      • PD 1 antibody
      • PD-1 antibody
      • PD1 antibody
      • PDCD 1 antibody
      • PDCD1 antibody
      • PDCD1_HUMAN antibody
      • Programmed cell death 1 antibody
      • Programmed cell death 1 protein antibody
      • Programmed cell death protein 1 antibody
      • Protein PD 1 antibody
      • Protein PD-1 antibody
      • SLEB2 antibody
      • Systemic lupus erythematosus susceptibility 2 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [NAT105] (ab52587)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [NAT105] (ab52587)

      IHC image of PD1 staining in normal human tonsil formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab52587 at 5 µg/ml for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [NAT105] (ab52587)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [NAT105] (ab52587)
      Double immunofluorescence staining of CD3 (green) and PD1 (red) on paraffin embedded tonsil.
    • Flow Cytometry - Anti-PD1 antibody [NAT105] (ab52587)
      Flow Cytometry - Anti-PD1 antibody [NAT105] (ab52587)

      Sample: Human tonsil cell extract.
      Dilution: ab52587 antibody was used as 1/200 in 1x106 cells/tube.
      Anti-CD4 antibody was used as 1/200 dilution.
      Anti-CD8 antibody was used as 1/200 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [NAT105] (ab52587)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [NAT105] (ab52587)Image courtesy of an AbReview submitted by Dr Hajnalka Rajnai

      Formaldehyde-fixed, paraffin-embedded human follicular lymphoma tissue stained for PD1 with ab52587 at 1/100 dilution in immunohistochemical analysis.

      See Abreview

    • Western blot - Anti-PD1 antibody [NAT105] (ab52587)
      Western blot - Anti-PD1 antibody [NAT105] (ab52587)This image is courtesy of an anonymous collaborator.
      Anti-PD1 antibody [NAT105] (ab52587) at 1/50 dilution + YT cell line extracts

      Predicted band size: 32 kDa
      Observed band size: 47 kDa why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [NAT105] (ab52587)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [NAT105] (ab52587)Schmidt et al PLoS One. 2015 Aug 27;10(8):e0136023. doi: 10.1371/journal.pone.0136023. eCollection 2015. Fig 1. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

      Representative immunohistochemical staining results for PD1 using ab52587 at a 1/50 dilution in human formalin-fixed, paraffin-embedded tissue specimens.

      Panel A: Normal lung tissue, negative control;

      Panel B: Tonsillar tissue, positive control;

      Panel C: PD1-negative tumor infiltrating lymphocytes;

      Panel D: PD1-positive tumor infiltrating lymphocytes in squamous cell carcinomas).

      Image from PMID: 26313362

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [NAT105] (ab52587)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [NAT105] (ab52587)Kim et al PLoS One. 2013 Dec 11;8(12):e82870. doi: 10.1371/journal.pone.0082870. eCollection 2013. Fig 1. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

      Immunohistochemical analysis of various soft tissue sarcomas staining PD1 using ab52587 at a 1/50 dilution.

      Arrows indicate PD1 positive lymphocytes.

      Image from PMID: 24349382

    • Flow Cytometry - Anti-PD1 antibody [NAT105] (ab52587)
      Flow Cytometry - Anti-PD1 antibody [NAT105] (ab52587)

      Overlay histogram showing MOLT-4 (Human lymphoblastic leukemia cell line) cells stained with ab52587 (red line). Live cells were incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab52587, 1/100 dilution) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150117) at 1/2000 dilution for 30 min at 4°C.

      A mouse IgG1 isotype control antibody  (ab170190) was used at the same concentration and conditions as the primary antibody (black line). Unlabeled sample (blue line) was also used as a control.
       
      Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.
    • Immunocytochemistry/ Immunofluorescence - Anti-PD1 antibody [NAT105] (ab52587)
      Immunocytochemistry/ Immunofluorescence - Anti-PD1 antibody [NAT105] (ab52587)

      MOLT-4 (Human lymphoblastic leukemia cell line) cells stained for PD1 (colored green) using ab52587 in ICC/IF.

      Cells were fixed with 100% methanol (5 min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% Tween-20 for 1 hour at room temperature to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with ab52587 at 10 µg/ml and ab6046 (Rabbit polyclonal to beta Tubulin - Loading Control) at 1 µg/ml overnight at +4°C. The secondary antibodies used were Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150117) secondary antibody used at 1 µg/ml (colored green) and Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150080) secondary antibody (pseudo-colored red) used at 2 µg/ml for 1 hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43 µM for 1 hour at room temperature.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [NAT105] (ab52587)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [NAT105] (ab52587)This image is from PubMedId: 27777963. Kaewkangsadan V et al. (2016) Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

      Immunohistochemical analysis of human large and locally advanced breast cancers staining PD1 using ab52587.

      (Panel a) Low level of PD-1+ T cell infiltration.

      (Panel b) high level of PD-1+ T cell infiltration. (Itu: intratumoral Str: stromal).

      Image data from PMID: 27777963

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [NAT105] (ab52587)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [NAT105] (ab52587)
      Double immunoenzymatic staining of Ki67 (brown) and PD1 (red) on paraffin embedded tonsil.
    • Immunohistochemistry (Frozen sections) - Anti-PD1 antibody [NAT105] (ab52587)
      Immunohistochemistry (Frozen sections) - Anti-PD1 antibody [NAT105] (ab52587)

      Human tonsil tissue stained for PD1 with ab52587 incubated for 30 mins at a 1/100 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Frozen sections) - Anti-PD1 antibody [NAT105] (ab52587)
      Immunohistochemistry (Frozen sections) - Anti-PD1 antibody [NAT105] (ab52587)This image is courtesy of an anonymous Abreview

      Immunohistochemical analysis of frozen human liver tissue labeling PD1 with ab52587 at 1/50 dilution.

      See Abreview

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [NAT105] (ab52587)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [NAT105] (ab52587)Argüello et al PLoS Negl Trop Dis. 2014 Aug 21;8(8):e2989. doi: 10.1371/journal.pntd.0002989. eCollection 2014 Aug. Fig 2. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

      Expression of markers of T cell differentiation and degree of inflammation in the heart of chronically T. cruzi-infected subjects with severe cardiomyopathy.

      A–D, left panel: representative photos of CD45RO, CD27, PD1 and CD57 expression, respectively.

      Panel C shown.

      Image data from PMID: 25144227

    Protocols

    • Flow cytometry protocols
    • Immunoprecipitation protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (192)

    Publishing research using ab52587? Please let us know so that we can cite the reference in this datasheet.

    ab52587 has been referenced in 192 publications.

    • Sakimura S  et al. Impaired tumor immune response in metastatic tumors is a selective pressure for neutral evolution in CRC cases. PLoS Genet 17:e1009113 (2021). PubMed: 33476333
    • Del Valle L  et al. Role of EIF4G1 network in non-small cell lung cancers (NSCLC) cell survival and disease progression. J Cell Mol Med 25:2795-2805 (2021). PubMed: 33539648
    • Goldmann T  et al. PD-L1 amplification is associated with an immune cell rich phenotype in squamous cell cancer of the lung. Cancer Immunol Immunother 70:2577-2587 (2021). PubMed: 33576873
    • Jin MH  et al. WEE1 inhibition reverses trastuzumab resistance in HER2-positive cancers. Gastric Cancer 24:1003-1020 (2021). PubMed: 33723720
    • Pfister D  et al. NASH limits anti-tumour surveillance in immunotherapy-treated HCC. Nature 592:450-456 (2021). PubMed: 33762733
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Immunohistochemistry (Frozen sections) abreview for Anti-PD1 antibody [NAT]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Liver)
    Specification
    Liver
    Fixative
    Acetone/Methanol 1:2
    Permeabilization
    Yes - TBS + 0,1% TritonX100
    Blocking step
    Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 15 2010

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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