Question (70921) | Anti-PD1 antibody [NAT105] (ab52587)

Go to datasheet (ab52587)

Question

Since ab52587 is a mouse monoclonal I was wondering what secondary to use? We would like to titrate the Ab and am not sure how to go about doing it.

Answer

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You may want to consider the following secondary antibodies:

https://www.abcam.com/donkey-fab2-polyclonal-secondary-antibody-to-mouse-igg-hl-alexa-fluorreg-488-pre-adsorbed-ab150101.html

https://www.abcam.com/donkey-fab2-polyclonal-secondary-antibody-to-mouse-igg-hl-alexa-fluor-647-pre-adsorbed-ab150103.html

https://www.abcam.com/goat-polyclonal-secondary-antibody-to-mouse-igg1-gamma-chain-cy5--pre-adsorbed-ab136127.html

https://www.abcam.com/goat-polyclonal-secondary-antibody-to-mouse-igg1-heavy-chain-fitc-ab97239.html

https://www.abcam.com/goat-polyclonal-secondary-antibody-to-mouse-igg1-heavy-chain-allophycocyanin-pre-adsorbed-ab130786.html

If you are concerned about the titration of the antibody, I would suggest trying the experiment with a range of different concentrations.

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