Immunohistochemistry (Frozen sections) abreview for Anti-PD1 antibody [NAT]

Good
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Liver)
Specification
Liver
Fixative
Acetone/Methanol 1:2
Permeabilization
Yes - TBS + 0,1% TritonX100
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 20°C

Other product details

Dilution
1/50
Incubation time
30 minute(s) · Temperature: 20°C · Diluent: PBS+BSA+Tween

Secondary antibody

Name
Non-Abcam antibody was used: Rat Anti-Mouse/Rabbit-Polymer (HRP)
Host species: Rat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Additional data

Additional Notes
Blocking with 1% Hydrogen Peroxide.
Image: Liver (human)

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Submitted Dec 15 2010

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