Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PD1 antibody [NAT105]

Good
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Prostate Cancer)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer pH 6.0
Permeabilization
No
Specification
Prostate Cancer
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative
Formaldehyde

Other product details

Incubation time
30 minute(s) · Temperature: 23°C
Concentration
20 µg/ml

Secondary antibody

Dilution
1/20000
Name
Non-Abcam antibody was used: IgG goat anti mouse-HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Additional data

Additional Notes
PD1 (DAB) / CK-Cocktail (Fast Red) double staining

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Submitted Nov 12 2015

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