Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-PD1 antibody [NAT105]

Inconclusive
Abreviews
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Sheep Tissue sections (lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
No
Specification
lung
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 25% · Temperature: 22°C
Fixative
Formaldehyde

Other product details

Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBST20
Dilution
1/100

Secondary antibody

Dilution
1/2
Name
Non-Abcam antibody was used: DAKO EnVision+System-HRP labelled Polymer Anti-mou
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Additional data

Additional Notes
It did not work. Non staining
Abcam response
Thank you for taking part in the AbTrial program and your honest feedback. As the antibody had not yet been tested in this species we were unsure what the result might be and we appreciate the time and effort that the reviewer has put in to testing this product in this species. We encourage all our customers to contact us should they wish to test any of our products in untested species or applications, as these may qualify for our AbTrial testing program

Ms. Anna Karagianni

Verified customer

Submitted Aug 11 2017

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