Recombinant
RabMAb

Anti-PD1 antibody [NAT105] - BSA and Azide free (ab201811)

Overview

  • Product name
    Anti-PD1 antibody [NAT105] - BSA and Azide free
    See all PD1 primary antibodies
  • Description
    Mouse monoclonal [NAT105] to PD1 - BSA and Azide free
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Tissue, cells or virus corresponding to Human PD1. TY cells (human T/NK cell Leukemia).
    Database link: Q15116

  • Positive control
    • IHC-P: Human tonsil tissue.
  • General notes

    ab201811 is a PBS-only buffer format of ab234444. Please refer to ab234444 for recommended dilutions, protocols, and image data.

     

Properties

Applications

Our Abpromise guarantee covers the use of ab201811 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 32 kDa.

See Western blot protocol.

 

Flow Cyt Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

Perform heat-mediated antigen retrieval using Sodium citrate buffer (pH 6.0), 20 mins.

 

Target

  • Function
    Possible cell death inducer, in association with other factors.
  • Involvement in disease
    Genetic variation in PDCD1 is associated with susceptibility to systemic lupus erythematosus type 2 (SLEB2) [MIM:605218]. Systemic lupus erythematosus is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
  • Sequence similarities
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Developmental stage
    Induced at programmed cell death.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD279 antibody
    • CD279 antigen antibody
    • hPD 1 antibody
    • hPD l antibody
    • hPD-1 antibody
    • hSLE1 antibody
    • PD 1 antibody
    • PD-1 antibody
    • PD1 antibody
    • PDCD 1 antibody
    • PDCD1 antibody
    • PDCD1_HUMAN antibody
    • Programmed cell death 1 antibody
    • Programmed cell death 1 protein antibody
    • Programmed cell death protein 1 antibody
    • Protein PD 1 antibody
    • Protein PD-1 antibody
    • SLEB2 antibody
    • Systemic lupus erythematosus susceptibility 2 antibody
    see all

Images

  • Flow cytometric analysis of MOLT-4 (human lymphoblastic leukemia cell line) cell line treated with ionomycin (500 ng/ml, 24h) and PMA (10 ng/ml, 24h) labeling PD1 with ab234444 at 1/1000 dilution (red) and an untreated control (green) compared with a Mouse monoclonal IgG1 (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab234444).

     

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling PD1 with ab234444 at 1/50 dilution, followed by Rabbit Anti-Mouse IgG + Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on T cells of human tonsil germinal center is observed. Performed on a Leica Biosystems BOND instrument. Counter stained with hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab234444).

References

ab201811 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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