The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use 1µg for 105-8 cells.
ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
Possible cell death inducer, in association with other factors.
Involvement in disease
Genetic variation in PDCD1 is associated with susceptibility to systemic lupus erythematosus type 2 (SLEB2) [MIM:605218]. Systemic lupus erythematosus is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
Flow cytometric analysis of 3-day ConA-stimulated BALB/c splenocytes (right histogram) labeling PD1 with ab171265 with Rat IgG2a K Isotype Control (open histogram). Total viable cells were used for analysis.