Overview

  • Product name
    Anti-PD1 antibody [SP269]
    See all PD1 primary antibodies
  • Description
    Rabbit monoclonal [SP269] to PD1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human PD1 aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: Q15116

  • Positive control
    • Flow Cyt: THP-1 cells. IHC-P: Human tonsil tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab227681 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100.

Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol.

Primary incubation for 30 minutes at room temperature.

Flow Cyt 1/400.

Primary incubation for 30 minutes at 4°C.

Target

  • Function
    Possible cell death inducer, in association with other factors.
  • Involvement in disease
    Genetic variation in PDCD1 is associated with susceptibility to systemic lupus erythematosus type 2 (SLEB2) [MIM:605218]. Systemic lupus erythematosus is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
  • Sequence similarities
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Developmental stage
    Induced at programmed cell death.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD279 antibody
    • CD279 antigen antibody
    • hPD 1 antibody
    • hPD l antibody
    • hPD-1 antibody
    • hSLE1 antibody
    • PD 1 antibody
    • PD-1 antibody
    • PD1 antibody
    • PDCD 1 antibody
    • PDCD1 antibody
    • PDCD1_HUMAN antibody
    • Programmed cell death 1 antibody
    • Programmed cell death 1 protein antibody
    • Programmed cell death protein 1 antibody
    • Protein PD 1 antibody
    • Protein PD-1 antibody
    • SLEB2 antibody
    • Systemic lupus erythematosus susceptibility 2 antibody
    see all

Images

  • Flow cytometric analysis of THP-1 (human monocytic leukemia cell line) cell line labeling PD1 with ab227681 at 1/400 dilution (green) compared with a Rabbit IgG negative control (blue). 

  • Formalin-fixed, paraffin-embedded human tonsil tissue stained for PD1 using ab227681 at 1/100 dilution in immunohistochemical analysis.

References

This product has been referenced in:
  • Zheng B  et al. PD-1 axis expression in musculoskeletal tumors and antitumor effect of nivolumab in osteosarcoma model of humanized mouse. J Hematol Oncol 11:16 (2018). Read more (PubMed: 29409495) »
  • Obara W  et al. Phase I clinical trial of cell division associated 1 (CDCA1) peptide vaccination for castration resistant prostate cancer. Cancer Sci 108:1452-1457 (2017). Read more (PubMed: 28498618) »
See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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