Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP269] to PD1 - BSA and Azide free
- Suitable for: IHC-P, Flow Cyt
- Reacts with: Human
Product nameAnti-PD1 antibody [SP269] - BSA and Azide free
See all PD1 primary antibodies
DescriptionRabbit monoclonal [SP269] to PD1 - BSA and Azide free
Tested applicationsSuitable for: IHC-P, Flow Cytmore details
Species reactivityReacts with: Human
Synthetic peptide within Human PD1 aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: Q15116
- Flow Cyt: THP-1 cells. IHC-P: Human tonsil tissue.
FOR RESEARCH USE ONLY. For commercial use, please contact firstname.lastname@example.org.
Ab243937 is the carrier-free version of ab227681. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab243937 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A/G purified
Purification notesPurified from TCS by protein A/G.
Our Abpromise guarantee covers the use of ab243937 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.
Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol.
Primary incubation for 30 minutes at room temperature.
|Flow Cyt||Use at an assay dependent concentration.
Primary incubation for 30 minutes at 4°C.
FunctionPossible cell death inducer, in association with other factors.
Involvement in diseaseGenetic variation in PDCD1 is associated with susceptibility to systemic lupus erythematosus type 2 (SLEB2) [MIM:605218]. Systemic lupus erythematosus is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
Developmental stageInduced at programmed cell death.
- Information by UniProt
- CD279 antibody
- CD279 antigen antibody
- hPD 1 antibody
Flow cytometric analysis of THP-1 (human monocytic leukemia cell line) cell line labeling PD1 with ab227681 at 1/400 dilution (green) compared with a Rabbit IgG negative control (blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab227681).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab243937 has not yet been referenced specifically in any publications.