Anti-PDCD10/CCM3 antibody (ab180706)
Key features and details
- Rabbit polyclonal to PDCD10/CCM3
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-PDCD10/CCM3 antibody -
Description
Rabbit polyclonal to PDCD10/CCM3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Human PDCD10/CCM3.
Database link: Q9BUL8 -
Positive control
- Mouse fetal brain, HeLa and K562 cell extracts; Human renal cancer tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab180706 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 25 kDa.
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IHC-P |
1/50 - 1/100.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 25 kDa. |
IHC-P
1/50 - 1/100. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Promotes cell proliferation. Modulates apoptotic pathways. Increases mitogen-activated protein kinase activity and MST4 activity. Important for cell migration, and for normal structure and assembly of the Golgi complex. Important for KDR/VEGFR2 signaling. Increases the stability of KDR/VEGFR2 and prevents its breakdown. Required for normal cardiovascular development. Required for normal angiogenesis, vasculogenesis and hematopoiesis during embryonic development. -
Tissue specificity
Ubiquitous. -
Involvement in disease
Defects in PDCD10 are the cause of cerebral cavernous malformations type 3 (CCM3) [MIM:603285]. Cerebral cavernous malformations (CCMs) are congenital vascular anomalies of the central nervous system that can result in hemorrhagic stroke, seizures, recurrent headaches, and focal neurologic deficits. CCMs have an incidence of 0.1%-0.5% in the general population and usually present clinically during the 3rd to 5th decade of life. The lesions are characterized by grossly enlarged blood vessels consisting of a single layer of endothelium and without any intervening neural tissue, ranging in diameter from a few millimeters to several centimeters. -
Sequence similarities
Belongs to the PDCD10 family. -
Cellular localization
Cytoplasm. Golgi apparatus membrane. Cell membrane. Partially co-localizes with endogenous PXN at the leading edges of migrating cells. - Information by UniProt
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Database links
- Entrez Gene: 11235 Human
- Entrez Gene: 56426 Mouse
- Omim: 609118 Human
- SwissProt: Q9BUL8 Human
- SwissProt: Q8VE70 Mouse
- Unigene: 478150 Human
- Unigene: 316473 Mouse
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Alternative names
- Apoptosis related protein 15 antibody
- CCM3 antibody
- Cerebral cavernous malformations 3 protein antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded Human renal cancer tissue labeling PDCD10/CCM3 with ab180706 at 1/50 dilution.
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All lanes : Anti-PDCD10/CCM3 antibody (ab180706) at 1/500 dilution
Lane 1 : Mouse fetal brain cell extract
Lane 2 : HeLa cell extract
Lane 3 : K562 cell extract
Predicted band size: 25 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab180706 has been referenced in 2 publications.
- Wang K et al. ATPIF1 maintains normal mitochondrial structure which is impaired by CCM3 deficiency in endothelial cells. Cell Biosci 11:11 (2021). PubMed: 33422124
- Zeng H et al. Genome-wide CRISPR screening reveals genetic modifiers of mutant EGFR dependence in human NSCLC. Elife 8:N/A (2019). PubMed: 31741433