Overview

  • Product name

  • Description

    Rabbit polyclonal to PDCD10/CCM3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Dog, Xenopus laevis, Chimpanzee, Zebrafish, Orangutan, Xenopus tropicalis
  • Immunogen

    Synthetic peptide within Human PDCD10/CCM3 aa 75-125. The exact sequence is proprietary.
    Database link: Q9BUL8

  • Positive control

    • IP: HeLa whole cell lysate.
  • General notes

    ab226181 has not performed satisfactorily when used for WB of PDCD10/CCM3 in crude preparations (e.g. whole cell lysate).  This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of PDCD10/CCM3.

     This product was previously labelled as PDCD10

     

Properties

Applications

Our Abpromise guarantee covers the use of ab226181 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function

      Promotes cell proliferation. Modulates apoptotic pathways. Increases mitogen-activated protein kinase activity and MST4 activity. Important for cell migration, and for normal structure and assembly of the Golgi complex. Important for KDR/VEGFR2 signaling. Increases the stability of KDR/VEGFR2 and prevents its breakdown. Required for normal cardiovascular development. Required for normal angiogenesis, vasculogenesis and hematopoiesis during embryonic development.
    • Tissue specificity

      Ubiquitous.
    • Involvement in disease

      Defects in PDCD10 are the cause of cerebral cavernous malformations type 3 (CCM3) [MIM:603285]. Cerebral cavernous malformations (CCMs) are congenital vascular anomalies of the central nervous system that can result in hemorrhagic stroke, seizures, recurrent headaches, and focal neurologic deficits. CCMs have an incidence of 0.1%-0.5% in the general population and usually present clinically during the 3rd to 5th decade of life. The lesions are characterized by grossly enlarged blood vessels consisting of a single layer of endothelium and without any intervening neural tissue, ranging in diameter from a few millimeters to several centimeters.
    • Sequence similarities

      Belongs to the PDCD10 family.
    • Cellular localization

      Cytoplasm. Golgi apparatus membrane. Cell membrane. Partially co-localizes with endogenous PXN at the leading edges of migrating cells.
    • Information by UniProt
    • Database links

    • Alternative names

      • Apoptosis related protein 15 antibody
      • CCM3 antibody
      • Cerebral cavernous malformations 3 protein antibody
      • MGC1212 antibody
      • MGC24477 antibody
      • PDC10_HUMAN antibody
      • PDCD 10 antibody
      • PDCD10 antibody
      • Programmed cell death 10 antibody
      • Programmed cell death protein 10 antibody
      • TF 1 cell apoptosis related protein 15 antibody
      • TF-1 cell apoptosis-related protein 15 antibody
      • TFAR15 antibody
      see all

    Images

    • PDCD10/CCM3 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226181 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226181 at 0.4 µg/ml.

      Lane 1: ab226181 IP in HeLa whole cell lysate.

      Lane 2: Control IgG IP in HeLa whole cell lysate.

      Detection:  Chemiluminescence with exposure time of 30 seconds.

    References

    ab226181 has not yet been referenced specifically in any publications.

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