Key features and details
- Rabbit polyclonal to PDCD10/CCM3
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PDCD10/CCM3 antibody
See all PDCD10/CCM3 primary antibodies
DescriptionRabbit polyclonal to PDCD10/CCM3
Tested applicationsSuitable for: IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Dog, Xenopus laevis, Chimpanzee, Zebrafish, Orangutan, Xenopus tropicalis
Synthetic peptide within Human PDCD10/CCM3 aa 75-125. The exact sequence is proprietary.
Database link: Q9BUL8
- IP: HeLa whole cell lysate.
ab226181 has not performed satisfactorily when used for WB of PDCD10/CCM3 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of PDCD10/CCM3.
This product was previously labelled as PDCD10
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab226181 was affinity purified using an epitope specific to PDCD10/CCM3 immobilized on solid support.
Our Abpromise guarantee covers the use of ab226181 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
FunctionPromotes cell proliferation. Modulates apoptotic pathways. Increases mitogen-activated protein kinase activity and MST4 activity. Important for cell migration, and for normal structure and assembly of the Golgi complex. Important for KDR/VEGFR2 signaling. Increases the stability of KDR/VEGFR2 and prevents its breakdown. Required for normal cardiovascular development. Required for normal angiogenesis, vasculogenesis and hematopoiesis during embryonic development.
Involvement in diseaseDefects in PDCD10 are the cause of cerebral cavernous malformations type 3 (CCM3) [MIM:603285]. Cerebral cavernous malformations (CCMs) are congenital vascular anomalies of the central nervous system that can result in hemorrhagic stroke, seizures, recurrent headaches, and focal neurologic deficits. CCMs have an incidence of 0.1%-0.5% in the general population and usually present clinically during the 3rd to 5th decade of life. The lesions are characterized by grossly enlarged blood vessels consisting of a single layer of endothelium and without any intervening neural tissue, ranging in diameter from a few millimeters to several centimeters.
Sequence similaritiesBelongs to the PDCD10 family.
Cellular localizationCytoplasm. Golgi apparatus membrane. Cell membrane. Partially co-localizes with endogenous PXN at the leading edges of migrating cells.
- Information by UniProt
- Apoptosis related protein 15 antibody
- CCM3 antibody
- Cerebral cavernous malformations 3 protein antibody
PDCD10/CCM3 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226181 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226181 at 0.4 µg/ml.
Lane 1: ab226181 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226181 has not yet been referenced specifically in any publications.