Overview

  • Product name

  • Description

    Goat polyclonal to PDCD4
  • Host species

    Goat
  • Specificity

    ab190169 is expected to recognize all reported isoforms (NP_055271.2; NP_663314.1; NP_001186421.1).
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Goat, Horse, Chicken, Guinea pig, Hamster, Cow, Dog, Pig, Chimpanzee, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human PDCD4 aa 210-221 (internal sequence) (Cysteine residue). (NP_055271.2).
    Sequence:

    EGKASHREMTSK


    Database link: Q53EL6

  • Positive control

    • HeLa cell lysate; Human prostate tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab190169 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 51 kDa).
IHC-P Use a concentration of 5 µg/ml.

Target

  • Function

    Inhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA.
  • Tissue specificity

    Up-regulated in proliferative cells. Highly expressed in epithelial cells of the mammary gland. Reduced expression in lung cancer and colon carcinoma.
  • Sequence similarities

    Belongs to the PDCD4 family.
    Contains 2 MI domains.
  • Domain

    Binds EIF4A1 via both MI domains.
  • Post-translational
    modifications

    Polyubiquitinated, leading to its proteasomal degradation. Rapidly degraded in response to mitogens. Phosphorylation of the phosphodegron promotes interaction with BTRC and proteasomal degradation.
  • Cellular localization

    Nucleus. Cytoplasm. Shuttles between the nucleus and cytoplasm. Predominantly nuclear under normal growth conditions, and when phosphorylated at Ser-457. Exported from the nucleus in the absence of serum.
  • Information by UniProt
  • Database links

  • Alternative names

    • Death up-regulated gene protein antibody
    • Dug antibody
    • H731 antibody
    • Ma3 antibody
    • MGC33046 antibody
    • MGC33047 antibody
    • Neoplastic transformation inhibitor antibody
    • Neoplastic transformation inhibitor protein antibody
    • Nuclear antigen H731 antibody
    • Nuclear antigen H731 like antibody
    • Nuclear antigen H731 like protein antibody
    • Nuclear antigen H731-like antibody
    • PDCD 4 antibody
    • Pdcd4 antibody
    • PDCD4_HUMAN antibody
    • Programmed cell death 4 antibody
    • programmed cell death 4 (neoplastic transformation inhibitor) antibody
    • Programmed cell death protein 4 antibody
    • Protein 197/15a antibody
    • Protein MA-3 antibody
    • Tis antibody
    • Topoisomerase-inhibitor suppressed protein antibody
    see all

Images

  • Anti-PDCD4 antibody (ab190169) at 0.1 µg/ml + HeLa cell lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 51 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?



    Primary incubation was 1 hour.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human prostate tissue labeling PDCD4 with ab190169 at 5 µg/ml.

References

ab190169 has not yet been referenced specifically in any publications.

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