Key features and details
- Goat polyclonal to PDCD4
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PDCD4 antibody
See all PDCD4 primary antibodies
DescriptionGoat polyclonal to PDCD4
Specificityab190169 is expected to recognize all reported isoforms (NP_055271.2; NP_663314.1; NP_001186421.1).
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Goat, Horse, Chicken, Guinea pig, Hamster, Cow, Dog, Pig, Chimpanzee, Orangutan
- HeLa cell lysate; Human prostate tissue.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab190169 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 51 kDa).|
|IHC-P||Use a concentration of 5 µg/ml.|
FunctionInhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA.
Tissue specificityUp-regulated in proliferative cells. Highly expressed in epithelial cells of the mammary gland. Reduced expression in lung cancer and colon carcinoma.
Sequence similaritiesBelongs to the PDCD4 family.
Contains 2 MI domains.
DomainBinds EIF4A1 via both MI domains.
modificationsPolyubiquitinated, leading to its proteasomal degradation. Rapidly degraded in response to mitogens. Phosphorylation of the phosphodegron promotes interaction with BTRC and proteasomal degradation.
Cellular localizationNucleus. Cytoplasm. Shuttles between the nucleus and cytoplasm. Predominantly nuclear under normal growth conditions, and when phosphorylated at Ser-457. Exported from the nucleus in the absence of serum.
- Information by UniProt
- Death up-regulated gene protein antibody
- Dug antibody
- H731 antibody
Anti-PDCD4 antibody (ab190169) at 0.1 µg/ml + HeLa cell lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 51 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human prostate tissue labeling PDCD4 with ab190169 at 5 µg/ml.
ab190169 has not yet been referenced specifically in any publications.