Overview

  • Product name

  • Description

    Rabbit polyclonal to PDCD4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Orangutan
  • Immunogen

    Synthetic peptide within Human PDCD4 aa 100-150. The exact sequence is proprietary. NP_055271.2
    Database link: Q53EL6

  • Positive control

    • IP: HeLa whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab245418 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function

      Inhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA.
    • Tissue specificity

      Up-regulated in proliferative cells. Highly expressed in epithelial cells of the mammary gland. Reduced expression in lung cancer and colon carcinoma.
    • Sequence similarities

      Belongs to the PDCD4 family.
      Contains 2 MI domains.
    • Domain

      Binds EIF4A1 via both MI domains.
    • Post-translational
      modifications

      Polyubiquitinated, leading to its proteasomal degradation. Rapidly degraded in response to mitogens. Phosphorylation of the phosphodegron promotes interaction with BTRC and proteasomal degradation.
    • Cellular localization

      Nucleus. Cytoplasm. Shuttles between the nucleus and cytoplasm. Predominantly nuclear under normal growth conditions, and when phosphorylated at Ser-457. Exported from the nucleus in the absence of serum.
    • Information by UniProt
    • Database links

    • Alternative names

      • Death up-regulated gene protein antibody
      • Dug antibody
      • H731 antibody
      • Ma3 antibody
      • MGC33046 antibody
      • MGC33047 antibody
      • Neoplastic transformation inhibitor antibody
      • Neoplastic transformation inhibitor protein antibody
      • Nuclear antigen H731 antibody
      • Nuclear antigen H731 like antibody
      • Nuclear antigen H731 like protein antibody
      • Nuclear antigen H731-like antibody
      • PDCD 4 antibody
      • Pdcd4 antibody
      • PDCD4_HUMAN antibody
      • Programmed cell death 4 antibody
      • programmed cell death 4 (neoplastic transformation inhibitor) antibody
      • Programmed cell death protein 4 antibody
      • Protein 197/15a antibody
      • Protein MA-3 antibody
      • Tis antibody
      • Topoisomerase-inhibitor suppressed protein antibody
      see all

    Images

    • PDCD4 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245418 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using an alternative anti-PDCD4 antibody at 1 µg/ml.

      Lane 1: ab245418 IP in HeLa whole cell lysate.

      Lane 2: Control IgG IP in HeLa whole cell lysate.

      Detection: Chemiluminescence with exposure time of 30 seconds.

    References

    ab245418 has not yet been referenced specifically in any publications.

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