Recombinant Anti-PDCD4 antibody [EPR3432] (ab79405)
- Datasheet
- References (2)
- Protocols
Overview
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Product nameAnti-PDCD4 antibody [EPR3432]
See all PDCD4 primary antibodies -
DescriptionRabbit monoclonal [EPR3432] to PDCD4
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-P, ICC, Flow Cytmore details
Unsuitable for: IP -
Species reactivityReacts with: Mouse, Human
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Immunogen
Synthetic peptide within Human PDCD4 aa 400-500 (C terminal). The exact sequence is proprietary.
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Positive control
- Jurkat and HeLa whole cell lysate (ab150035); human breast carcinoma tissue.
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General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR3432
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab79405 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/5000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa). | |
| IHC-P | 1/1000 - 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| ICC | 1/250 - 1/500. | |
| Flow Cyt | 1/50. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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FunctionInhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA.
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Tissue specificityUp-regulated in proliferative cells. Highly expressed in epithelial cells of the mammary gland. Reduced expression in lung cancer and colon carcinoma.
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Sequence similaritiesBelongs to the PDCD4 family.
Contains 2 MI domains. -
DomainBinds EIF4A1 via both MI domains.
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Post-translational
modificationsPolyubiquitinated, leading to its proteasomal degradation. Rapidly degraded in response to mitogens. Phosphorylation of the phosphodegron promotes interaction with BTRC and proteasomal degradation. -
Cellular localizationNucleus. Cytoplasm. Shuttles between the nucleus and cytoplasm. Predominantly nuclear under normal growth conditions, and when phosphorylated at Ser-457. Exported from the nucleus in the absence of serum.
- Information by UniProt
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Database links
- Entrez Gene: 27250 Human
- Entrez Gene: 18569 Mouse
- Omim: 608610 Human
- SwissProt: Q53EL6 Human
- SwissProt: Q61823 Mouse
- Unigene: 711490 Human
- Unigene: 1605 Mouse
- Unigene: 375091 Mouse
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Alternative names
- Death up-regulated gene protein antibody
- Dug antibody
- H731 antibody
see all
Images
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![Western blot - Anti-PDCD4 antibody [EPR3432] (ab79405)](https://www.abcam.com/ps/products/79/ab79405/Images/ab79405-303085-ab79405KOWBJRIZ.jpg)
Western blot - Anti-PDCD4 antibody [EPR3432] (ab79405)Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: PDCD4 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: HEK293 whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab79405 observed at 52 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab79405 was shown to recognize PDCD4 in wild-type HAP1 cells as signal was lost at the expected MW in PDCD4 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and PDCD4 knockout samples were subjected to SDS-PAGE. ab79405 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-PDCD4 antibody [EPR3432] (ab79405)](https://www.abcam.com/ps/products/79/ab79405/Images/PDCD4-Primary-antibodies-ab79405-1.jpg)
Western blot - Anti-PDCD4 antibody [EPR3432] (ab79405)All lanes : Anti-PDCD4 antibody [EPR3432] (ab79405) at 1/10000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa
Additional bands at: 30 kDa. We are unsure as to the identity of these extra bands. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD4 antibody [EPR3432] (ab79405)](https://www.abcam.com/ps/products/79/ab79405/Images/PDCD4-Primary-antibodies-ab79405-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD4 antibody [EPR3432] (ab79405)ab79405 at 1/1000 dilution staining PDCD4 in human breast carcinoma by Immunohistochemistry using paraffin-embedded tissue.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Liang X et al. MicroRNA-16 suppresses the activation of inflammatory macrophages in atherosclerosis by targeting PDCD4. Int J Mol Med 37:967-75 (2016). Read more (PubMed: 26936421) »
- Wright EC et al. MicroRNA-21 and PDCD4 expression during in vitro oocyte maturation in pigs. Reprod Biol Endocrinol 14:21 (2016). Read more (PubMed: 27084064) »
