Product nameAnti-PDCD4 antibody [EPR3432]
See all PDCD4 primary antibodies
DescriptionRabbit monoclonal [EPR3432] to PDCD4
Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
Unsuitable for: IP
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human PDCD4 aa 400-500 (C terminal). The exact sequence is proprietary.
- WB: HeLa, HAP1, and Jurkat whole cell lysate. IHC-P: Human breast carcinoma tissue. ICC/IF: HeLa cells
Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab79405 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).|
|IHC-P||1/1000 - 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionInhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA.
Tissue specificityUp-regulated in proliferative cells. Highly expressed in epithelial cells of the mammary gland. Reduced expression in lung cancer and colon carcinoma.
Sequence similaritiesBelongs to the PDCD4 family.
Contains 2 MI domains.
DomainBinds EIF4A1 via both MI domains.
modificationsPolyubiquitinated, leading to its proteasomal degradation. Rapidly degraded in response to mitogens. Phosphorylation of the phosphodegron promotes interaction with BTRC and proteasomal degradation.
Cellular localizationNucleus. Cytoplasm. Shuttles between the nucleus and cytoplasm. Predominantly nuclear under normal growth conditions, and when phosphorylated at Ser-457. Exported from the nucleus in the absence of serum.
- Information by UniProt
- Death up-regulated gene protein antibody
- Dug antibody
- H731 antibody
All lanes : Anti-PDCD4 antibody [EPR3432] (ab79405) at 1/5000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : PDCD4 knockout HeLa cell lysate
Lane 3 : Wild-type HAP1 cell lysate
Lane 4 : PDCD4 knockout HAP1 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 52 kDa
Observed band size: 51 kDa why is the actual band size different from the predicted?
Lanes 1-4: Merged signal (red and green). Green - ab79405 observed at 51 kDa. Red - loading control ab8245 observed at 37 kDa.
ab79405 Anti-PDCD4 antibody [EPR3432] was shown to specifically react with PDCD4 in wild-type HeLa cells. Loss of signal was observed when knockout sample ab257278 was used. Wild-type and PDCD4 knockout samples were subjected to SDS-PAGE. ab79405 and Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (ab52866) were incubated overnight at 4°C at 1 in 5000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: PDCD4 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: HEK293 whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab79405 observed at 52 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab79405 was shown to recognize PDCD4 in wild-type HAP1 cells as signal was lost at the expected MW in PDCD4 knockout HAP1 cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and PDCD4 knockout samples were subjected to SDS-PAGE. ab79405 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling PDCD4 with purified ab79405 at 1/500 dilution (4 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL) was used as the secondary antibody only control.
All lanes : Anti-PDCD4 antibody [EPR3432] (ab79405) at 1/10000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa
Additional bands at: 30 kDa. We are unsure as to the identity of these extra bands.
ab79405 at 1/1000 dilution staining PDCD4 in human breast carcinoma by Immunohistochemistry using paraffin-embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
This product has been referenced in:
- Liang X et al. MicroRNA-16 suppresses the activation of inflammatory macrophages in atherosclerosis by targeting PDCD4. Int J Mol Med 37:967-75 (2016). Read more (PubMed: 26936421) »
- Wright EC et al. MicroRNA-21 and PDCD4 expression during in vitro oocyte maturation in pigs. Reprod Biol Endocrinol 14:21 (2016). Read more (PubMed: 27084064) »