• Product name

    Anti-PDCD4 (phospho S457) antibody [9G6]
    See all PDCD4 primary antibodies
  • Description

    Mouse monoclonal [9G6] to PDCD4 (phospho S457)
  • Host species

  • Specificity

    ab105998 is specific for Human PDCD4 protein phosphorylated at Ser457. Cross-reactivity with PDCD4 from other sources has not been determined.
  • Tested applications

    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Xenopus laevis
  • Immunogen

    Synthetic peptide corresponding to residues surrounding Ser457 of Human PDCD4 (NP_055271).

  • Positive control

    • NIH-3T3 cells stimulated with PDGF, Recombinant PDCD4 protein, Human small intestine tissue



Our Abpromise guarantee covers the use of ab105998 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 62 kDa.
ELISA 1/20000 - 1/100000.
IHC-P 1/1000 - 1/5000.


  • Function

    Inhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA.
  • Tissue specificity

    Up-regulated in proliferative cells. Highly expressed in epithelial cells of the mammary gland. Reduced expression in lung cancer and colon carcinoma.
  • Sequence similarities

    Belongs to the PDCD4 family.
    Contains 2 MI domains.
  • Domain

    Binds EIF4A1 via both MI domains.
  • Post-translational

    Polyubiquitinated, leading to its proteasomal degradation. Rapidly degraded in response to mitogens. Phosphorylation of the phosphodegron promotes interaction with BTRC and proteasomal degradation.
  • Cellular localization

    Nucleus. Cytoplasm. Shuttles between the nucleus and cytoplasm. Predominantly nuclear under normal growth conditions, and when phosphorylated at Ser-457. Exported from the nucleus in the absence of serum.
  • Information by UniProt
  • Database links

  • Alternative names

    • Death up-regulated gene protein antibody
    • Dug antibody
    • H731 antibody
    • Ma3 antibody
    • MGC33046 antibody
    • MGC33047 antibody
    • Neoplastic transformation inhibitor antibody
    • Neoplastic transformation inhibitor protein antibody
    • Nuclear antigen H731 antibody
    • Nuclear antigen H731 like antibody
    • Nuclear antigen H731 like protein antibody
    • Nuclear antigen H731-like antibody
    • PDCD 4 antibody
    • Pdcd4 antibody
    • PDCD4_HUMAN antibody
    • Programmed cell death 4 antibody
    • programmed cell death 4 (neoplastic transformation inhibitor) antibody
    • Programmed cell death protein 4 antibody
    • Protein 197/15a antibody
    • Protein MA-3 antibody
    • Tis antibody
    • Topoisomerase-inhibitor suppressed protein antibody
    see all


  • All lanes : Anti-PDCD4 (phospho S457) antibody [9G6] (ab105998) at 1/2000 dilution

    Lane 1 : NIH-3T3 cells stimulated with 30 ng/ml PDGF (5 min treatment)
    Lane 2 : Unstimulated (non-phosphorylated) NIH 3T3 cells

    All lanes : HRP conjugated Rabbit anti-Mouse IgG at 1/2000 dilution

    Predicted band size: 62 kDa

  • ab105998 at 1/1000 dilution staining PDCD4 in Human small intestine by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue.
    The image shows the localization of ab105998 as the precipitated red signal, with a hematoxylin purple nuclear counterstain.


This product has been referenced in:

  • Yuan H  et al. Downregulation of PDCD4 by miR-21 suppresses tumor transformation and proliferation in a nude mouse renal cancer model. Oncol Lett 14:3371-3378 (2017). Read more (PubMed: 28927090) »
  • Mao XH  et al. MicroRNA-21 regulates the ERK/NF-?B signaling pathway to affect the proliferation, migration, and apoptosis of human melanoma A375 cells by targeting SPRY1, PDCD4, and PTEN. Mol Carcinog 56:886-894 (2017). Read more (PubMed: 27533779) »
See all 2 Publications for this product

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