Key features and details
- Mouse monoclonal [9G6] to PDCD4 (phospho S457)
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG1
Product nameAnti-PDCD4 (phospho S457) antibody [9G6]
See all PDCD4 primary antibodies
DescriptionMouse monoclonal [9G6] to PDCD4 (phospho S457)
Specificityab105998 is specific for Human PDCD4 protein phosphorylated at Ser457. Cross-reactivity with PDCD4 from other sources has not been determined.
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Xenopus laevis
Synthetic peptide corresponding to residues surrounding Ser457 of Human PDCD4 (NP_055271).
- NIH-3T3 cells stimulated with PDGF, Recombinant PDCD4 protein, Human small intestine tissue
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride
Concentration information loading...
PurityProtein A purified
Purification notesPurified from concentrated tissue culture supernate by Protein A chromatography.
Our Abpromise guarantee covers the use of ab105998 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 62 kDa.|
|IHC-P||1/1000 - 1/5000.|
FunctionInhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA.
Tissue specificityUp-regulated in proliferative cells. Highly expressed in epithelial cells of the mammary gland. Reduced expression in lung cancer and colon carcinoma.
Sequence similaritiesBelongs to the PDCD4 family.
Contains 2 MI domains.
DomainBinds EIF4A1 via both MI domains.
modificationsPolyubiquitinated, leading to its proteasomal degradation. Rapidly degraded in response to mitogens. Phosphorylation of the phosphodegron promotes interaction with BTRC and proteasomal degradation.
Cellular localizationNucleus. Cytoplasm. Shuttles between the nucleus and cytoplasm. Predominantly nuclear under normal growth conditions, and when phosphorylated at Ser-457. Exported from the nucleus in the absence of serum.
- Information by UniProt
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All lanes : Anti-PDCD4 (phospho S457) antibody [9G6] (ab105998) at 1/2000 dilution
Lane 1 : NIH-3T3 cells stimulated with 30 ng/ml PDGF (5 min treatment)
Lane 2 : Unstimulated (non-phosphorylated) NIH 3T3 cells
All lanes : HRP conjugated Rabbit anti-Mouse IgG at 1/2000 dilution
Predicted band size: 62 kDa
ab105998 at 1/1000 dilution staining PDCD4 in Human small intestine by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue.
The image shows the localization of ab105998 as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
ab105998 has been referenced in 3 publications.
- Kenawy N et al. Conjunctival melanoma copy number alterations and correlation with mutation status, tumor features, and clinical outcome. Pigment Cell Melanoma Res 32:564-575 (2019). PubMed: 30672666
- Yuan H et al. Downregulation of PDCD4 by miR-21 suppresses tumor transformation and proliferation in a nude mouse renal cancer model. Oncol Lett 14:3371-3378 (2017). PubMed: 28927090
- Mao XH et al. MicroRNA-21 regulates the ERK/NF-?B signaling pathway to affect the proliferation, migration, and apoptosis of human melanoma A375 cells by targeting SPRY1, PDCD4, and PTEN. Mol Carcinog 56:886-894 (2017). PubMed: 27533779