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  1. Link

    pdcd6alg-2-antibody-ep3259-ab109181.pdf

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Cell Biology Apoptosis Extracellular Signals Death Ligands
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-PDCD6/ALG-2 antibody [EP3259] (ab109181)

  • Datasheet
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Western blot - Anti-PDCD6/ALG-2 antibody [EP3259] (ab109181)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD6/ALG-2 antibody [EP3259] (ab109181)
  • Western blot - Anti-PDCD6/ALG-2 antibody [EP3259] (ab109181)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD6/ALG-2 antibody [EP3259] (ab109181)
  • Anti-PDCD6/ALG-2 antibody [EP3259] (ab109181)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP3259] to PDCD6/ALG-2
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Protein
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Recombinant Human PDCD6/ALG-2 protein (ab104605)
Knockout
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Human PDCD6 (ALG-2) knockout HEK-293T cell line (ab266323)
Primary
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Anti-TIG3 antibody (ab96468)

View more associated products

Overview

  • Product name

    Anti-PDCD6/ALG-2 antibody [EP3259]
    See all PDCD6/ALG-2 primary antibodies
  • Description

    Rabbit monoclonal [EP3259] to PDCD6/ALG-2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Daudi, Jurkat, HeLa and 293T cell lysates. IHC-P: Human hepatoma and Human lung adenocarcinoma.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EP3259
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Extracellular Signals
    • Death Ligands
    • Signal Transduction
    • Signaling Pathway
    • Calcium Signaling
    • Calcium Binding Proteins

Associated products

  • Alternative Versions

    • Anti-PDCD6/ALG-2 antibody [EP3259] - BSA and Azide free (ab240941)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human PDCD6 (ALG-2) knockout HEK-293T cell line (ab266323)
  • KO cell lysates

    • Human PDCD6 (ALG-2) knockout HEK-293T cell lysate (ab258570)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
    • Jurkat whole cell lysate (ab7899)
  • Recombinant Protein

    • Recombinant Human PDCD6/ALG-2 protein (ab104605)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab109181 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/2000. Detects a band of approximately 22 kDa (predicted molecular weight: 21 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/1000 - 1/2000. Detects a band of approximately 22 kDa (predicted molecular weight: 21 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Application notes
Is unsuitable for Flow Cyt,ICC or IP.

Target

  • Function

    Calcium-binding protein required for T-cell receptor-, Fas-, and glucocorticoid-induced cell death. May mediate Ca(2+)-regulated signals along the death pathway (By similarity). Calcium-dependent adapter necessary for the association between PDCD6IP and TSG101. Interaction with DAPK1 can accelerate apoptotic cell death by increasing caspase-3 activity. May inhibit KDR/VEGFR2-dependent angiogenesis; the function involves inhibition of VEGF-induced phosphoprylation of the Akt signaling pathway. Seems to play a role in the regulation of the distribution and function of MCOLN1 in the endosomal pathway. Isoform 2 has a lower Ca(2+) affinity than isoform 1. Isoform 1 and, to a lesser extend, isoform 2, can stabilize SHISA5.
  • Sequence similarities

    Contains 5 EF-hand domains.
  • Cellular localization

    Nucleus membrane. Endoplasmic reticulum membrane. Nucleus. Endosome. Interaction with RBM22 induces relocalization from the cytoplasm to the nucleus. Translocated from the cytoplasm to the nucleus after heat shock cell treatment. Accumulates in cytoplasmic vesicle-like organelles after heat shock treatment, which may represent stress granules.
  • Target information above from: UniProt accession O75340 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 10016 Human
    • Entrez Gene: 18570 Mouse
    • Entrez Gene: 308061 Rat
    • Omim: 601057 Human
    • SwissProt: O75340 Human
    • SwissProt: P12815 Mouse
    • Unigene: 50823 Human
    • Unigene: 24254 Mouse
    • Alternative names

      • AIP1 antibody
      • ALG 2 antibody
      • ALG-2-interacting protein 1 antibody
      • ALG2 antibody
      • ALIX antibody
      • Apoptosis linked gene 2 antibody
      • Apoptosis linked gene 2 protein antibody
      • Apoptosis-linked gene 2 protein antibody
      • FLJ42309 antibody
      • FLJ46208 antibody
      • Hp95 antibody
      • KIAA1375 antibody
      • MA 3 antibody
      • MA3 antibody
      • MGC111017 antibody
      • MGC119050 antibody
      • MGC9123 antibody
      • PDCD 6 antibody
      • Pdcd6 antibody
      • PDCD6_HUMAN antibody
      • PEF 1B antibody
      • PEF1B antibody
      • Probable calcium binding protein ALG 2 antibody
      • Probable calcium binding protein ALG2 antibody
      • Probable calcium-binding protein ALG-2 antibody
      • Programmed cell death 6 antibody
      • Programmed cell death 6-interacting protein antibody
      • Programmed cell death protein 6 antibody
      • PS 2 antibody
      • PS2 antibody
      see all

    Images

    • Western blot - Anti-PDCD6/ALG-2 antibody [EP3259] (ab109181)
      Western blot - Anti-PDCD6/ALG-2 antibody [EP3259] (ab109181)
      All lanes : Anti-PDCD6/ALG-2 antibody [EP3259] (ab109181) at 1/1000 dilution

      Lane 1 : Wild-type HEK293T cell lysate
      Lane 2 : PDCD6 knockout HEK293T cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : Daudi cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

      Predicted band size: 21 kDa
      Observed band size: 22 kDa why is the actual band size different from the predicted?



      Lanes 1-4: Merged signal (red and green). Green - ab109181 observed at 22 kDa. Red - loading control ab8245 observed at 36 kDa.

       ab109181 Anti-PDCD6/ALG-2 antibody [EP3259] was shown to specifically react with PDCD6/ALG-2 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266323 (knockout cell lysate ab258570) was used. Wild-type and PDCD6/ALG-2 knockout samples were subjected to SDS-PAGE. ab109181 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD6/ALG-2 antibody [EP3259] (ab109181)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD6/ALG-2 antibody [EP3259] (ab109181)

      Immunohistochemical analysis of paraffin-embedded Human hepatoma using ab109181 at 1/100.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Western blot - Anti-PDCD6/ALG-2 antibody [EP3259] (ab109181)
      Western blot - Anti-PDCD6/ALG-2 antibody [EP3259] (ab109181)
      All lanes : Anti-PDCD6/ALG-2 antibody [EP3259] (ab109181) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 21 kDa
      Observed band size: 22 kDa why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD6/ALG-2 antibody [EP3259] (ab109181)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD6/ALG-2 antibody [EP3259] (ab109181)

      Immunohistochemical analysis of paraffin-embedded Human lung adenocarcinoma using ab109181 at 1/100.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-PDCD6/ALG-2 antibody [EP3259] (ab109181)
      Anti-PDCD6/ALG-2 antibody [EP3259] (ab109181)

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (3)

    Publishing research using ab109181? Please let us know so that we can cite the reference in this datasheet.

    ab109181 has been referenced in 3 publications.

    • Zhao L  et al. Expression level of NEAT1 differentiates benign and malignant thyroid nodules by regulating NEAT1/miR-9/PTEN and NEAT1/miR-124/PDCD6 signalling. Int J Mol Med 46:1661-1670 (2020). PubMed: 32901835
    • Zhang L  et al. MicroRNA-124-3p directly targets PDCD6 to inhibit metastasis in breast cancer. Oncol Lett 15:984-990 (2018). PubMed: 29387242
    • Kaul Z & Chakrabarti O Tumor susceptibility gene 101 regulates predisposition to apoptosis via ESCRT machinery accessory proteins. Mol Biol Cell 28:2106-2122 (2017). PubMed: 28539405

    Customer reviews and Q&As

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