Recombinant Anti-PDCD6/ALG-2 antibody [EP3259] (ab109181)
![Western blot - Anti-PDCD6/ALG-2 antibody [EP3259] (ab109181)](https://www.abcam.com/ps/products/109/ab109181/Images/ab109181-397681-anti-pdcd6alg-2-antibody-ep3259-western-blot-human-knockout-lysate.jpg "Western blot - Anti-PDCD6/ALG-2 antibody [EP3259] (ab109181)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP3259] to PDCD6/ALG-2
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PDCD6/ALG-2 antibody [EP3259]
See all PDCD6/ALG-2 primary antibodies -
Description
Rabbit monoclonal [EP3259] to PDCD6/ALG-2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Daudi, Jurkat, HeLa and 293T cell lysates. IHC-P: Human hepatoma and Human lung adenocarcinoma.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP3259 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109181 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/2000. Detects a band of approximately 22 kDa (predicted molecular weight: 21 kDa).
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Notes |
|---|
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WB
1/1000 - 1/2000. Detects a band of approximately 22 kDa (predicted molecular weight: 21 kDa). |
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IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Calcium-binding protein required for T-cell receptor-, Fas-, and glucocorticoid-induced cell death. May mediate Ca(2+)-regulated signals along the death pathway (By similarity). Calcium-dependent adapter necessary for the association between PDCD6IP and TSG101. Interaction with DAPK1 can accelerate apoptotic cell death by increasing caspase-3 activity. May inhibit KDR/VEGFR2-dependent angiogenesis; the function involves inhibition of VEGF-induced phosphoprylation of the Akt signaling pathway. Seems to play a role in the regulation of the distribution and function of MCOLN1 in the endosomal pathway. Isoform 2 has a lower Ca(2+) affinity than isoform 1. Isoform 1 and, to a lesser extend, isoform 2, can stabilize SHISA5. -
Sequence similarities
Contains 5 EF-hand domains. -
Cellular localization
Nucleus membrane. Endoplasmic reticulum membrane. Nucleus. Endosome. Interaction with RBM22 induces relocalization from the cytoplasm to the nucleus. Translocated from the cytoplasm to the nucleus after heat shock cell treatment. Accumulates in cytoplasmic vesicle-like organelles after heat shock treatment, which may represent stress granules. - Information by UniProt
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Database links
- Entrez Gene: 10016 Human
- Entrez Gene: 18570 Mouse
- Entrez Gene: 308061 Rat
- Omim: 601057 Human
- SwissProt: O75340 Human
- SwissProt: P12815 Mouse
- Unigene: 50823 Human
- Unigene: 24254 Mouse
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Alternative names
- AIP1 antibody
- ALG 2 antibody
- ALG-2-interacting protein 1 antibody
see all
Images
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Western blot - Anti-PDCD6/ALG-2 antibody [EP3259] (ab109181)All lanes : Anti-PDCD6/ALG-2 antibody [EP3259] (ab109181) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : PDCD6 knockout HEK293T cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Daudi cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 21 kDa
Observed band size: 22 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab109181 observed at 22 kDa. Red - loading control ab8245 observed at 36 kDa.
ab109181 Anti-PDCD6/ALG-2 antibody [EP3259] was shown to specifically react with PDCD6/ALG-2 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266323 (knockout cell lysate ab258570) was used. Wild-type and PDCD6/ALG-2 knockout samples were subjected to SDS-PAGE. ab109181 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD6/ALG-2 antibody [EP3259] (ab109181)](https://www.abcam.com/ps/products/109/ab109181/Images/ab109181-121751-anti-pdcd6-alg-2-antibody-ep3259-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD6/ALG-2 antibody [EP3259] (ab109181)Immunohistochemical analysis of paraffin-embedded Human hepatoma using ab109181 at 1/100.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-PDCD6/ALG-2 antibody [EP3259] (ab109181)](https://www.abcam.com/ps/products/109/ab109181/Images/ab109181-121752-anti-pdcd6-alg-2-antibody-ep3259-western-blot.jpg)
Western blot - Anti-PDCD6/ALG-2 antibody [EP3259] (ab109181)All lanes : Anti-PDCD6/ALG-2 antibody [EP3259] (ab109181) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 21 kDa
Observed band size: 22 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD6/ALG-2 antibody [EP3259] (ab109181)](https://www.abcam.com/ps/products/109/ab109181/Images/ab109181-121750-anti-pdcd6-alg-2-antibody-ep3259-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD6/ALG-2 antibody [EP3259] (ab109181)Immunohistochemical analysis of paraffin-embedded Human lung adenocarcinoma using ab109181 at 1/100.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-PDCD6/ALG-2 antibody [EP3259] (ab109181)](https://www.abcam.com/ps/products/109/ab109181/Images/ab109181-5-benefits-of-recombinant-antibodies.png)
Anti-PDCD6/ALG-2 antibody [EP3259] (ab109181)
Protocols
Datasheets and documents
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Datasheet download
References (3)
ab109181 has been referenced in 3 publications.
- Zhao L et al. Expression level of NEAT1 differentiates benign and malignant thyroid nodules by regulating NEAT1/miR-9/PTEN and NEAT1/miR-124/PDCD6 signalling. Int J Mol Med 46:1661-1670 (2020). PubMed: 32901835
- Zhang L et al. MicroRNA-124-3p directly targets PDCD6 to inhibit metastasis in breast cancer. Oncol Lett 15:984-990 (2018). PubMed: 29387242
- Kaul Z & Chakrabarti O Tumor susceptibility gene 101 regulates predisposition to apoptosis via ESCRT machinery accessory proteins. Mol Biol Cell 28:2106-2122 (2017). PubMed: 28539405
