Recombinant Anti-PDCD6/ALG-2 antibody [EP3259] (ab109181)
- Datasheet
- References (2)
- Protocols
Overview
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Product name
Anti-PDCD6/ALG-2 antibody [EP3259]
See all PDCD6/ALG-2 primary antibodies -
Description
Rabbit monoclonal [EP3259] to PDCD6/ALG-2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human PDCD6/ALG-2 aa 1-100. The exact sequence is proprietary.
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Positive control
- Jurkat, HeLa and 293T cell lysates, Human hepatoma and Human lung adenocarcinoma.
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General notes
Previously labeled as PDCD6.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP3259 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab109181 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/2000. Detects a band of approximately 22 kDa (predicted molecular weight: 21 kDa). | |
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Calcium-binding protein required for T-cell receptor-, Fas-, and glucocorticoid-induced cell death. May mediate Ca(2+)-regulated signals along the death pathway (By similarity). Calcium-dependent adapter necessary for the association between PDCD6IP and TSG101. Interaction with DAPK1 can accelerate apoptotic cell death by increasing caspase-3 activity. May inhibit KDR/VEGFR2-dependent angiogenesis; the function involves inhibition of VEGF-induced phosphoprylation of the Akt signaling pathway. Seems to play a role in the regulation of the distribution and function of MCOLN1 in the endosomal pathway. Isoform 2 has a lower Ca(2+) affinity than isoform 1. Isoform 1 and, to a lesser extend, isoform 2, can stabilize SHISA5. -
Sequence similarities
Contains 5 EF-hand domains. -
Cellular localization
Nucleus membrane. Endoplasmic reticulum membrane. Nucleus. Endosome. Interaction with RBM22 induces relocalization from the cytoplasm to the nucleus. Translocated from the cytoplasm to the nucleus after heat shock cell treatment. Accumulates in cytoplasmic vesicle-like organelles after heat shock treatment, which may represent stress granules. - Information by UniProt
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Database links
- Entrez Gene: 10016 Human
- Entrez Gene: 18570 Mouse
- Entrez Gene: 308061 Rat
- Omim: 601057 Human
- SwissProt: O75340 Human
- SwissProt: P12815 Mouse
- Unigene: 50823 Human
- Unigene: 24254 Mouse
see all -
Alternative names
- AIP1 antibody
- ALG 2 antibody
- ALG-2-interacting protein 1 antibody
see all
Images
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![Western blot - Anti-PDCD6/ALG-2 antibody [EP3259] (ab109181)](https://www.abcam.com/ps/products/109/ab109181/Images/ab109181-121752-anti-pdcd6-alg-2-antibody-ep3259-western-blot.jpg)
Western blot - Anti-PDCD6/ALG-2 antibody [EP3259] (ab109181)All lanes : Anti-PDCD6/ALG-2 antibody [EP3259] (ab109181) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 21 kDa
Observed band size: 22 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD6/ALG-2 antibody [EP3259] (ab109181)](https://www.abcam.com/ps/products/109/ab109181/Images/ab109181-121751-anti-pdcd6-alg-2-antibody-ep3259-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD6/ALG-2 antibody [EP3259] (ab109181)Immunohistochemical analysis of paraffin-embedded Human hepatoma using ab109181 at 1/100.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD6/ALG-2 antibody [EP3259] (ab109181)](https://www.abcam.com/ps/products/109/ab109181/Images/ab109181-121750-anti-pdcd6-alg-2-antibody-ep3259-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD6/ALG-2 antibody [EP3259] (ab109181)Immunohistochemical analysis of paraffin-embedded Human lung adenocarcinoma using ab109181 at 1/100.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Zhang L et al. MicroRNA-124-3p directly targets PDCD6 to inhibit metastasis in breast cancer. Oncol Lett 15:984-990 (2018). Read more (PubMed: 29387242) »
- Kaul Z & Chakrabarti O Tumor susceptibility gene 101 regulates predisposition to apoptosis via ESCRT machinery accessory proteins. Mol Biol Cell 28:2106-2122 (2017). Read more (PubMed: 28539405) »
