Overview

  • Product name

    Anti-PDCD6/ALG-2 antibody [EPR3260]
    See all PDCD6/ALG-2 primary antibodies
  • Description

    Rabbit monoclonal [EPR3260] to PDCD6/ALG-2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human PDCD6/ALG-2 aa 150-250. The exact sequence is proprietary.

  • Positive control

    • HeLa, Jurkat, and HepG2 whole cell lysate (ab7900).
  • General notes

     

     

    Previously labelled as PDCD6. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133326 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 22 kDa.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IHC-P.
  • Target

    • Function

      Calcium-binding protein required for T-cell receptor-, Fas-, and glucocorticoid-induced cell death. May mediate Ca(2+)-regulated signals along the death pathway (By similarity). Calcium-dependent adapter necessary for the association between PDCD6IP and TSG101. Interaction with DAPK1 can accelerate apoptotic cell death by increasing caspase-3 activity. May inhibit KDR/VEGFR2-dependent angiogenesis; the function involves inhibition of VEGF-induced phosphoprylation of the Akt signaling pathway. Seems to play a role in the regulation of the distribution and function of MCOLN1 in the endosomal pathway. Isoform 2 has a lower Ca(2+) affinity than isoform 1. Isoform 1 and, to a lesser extend, isoform 2, can stabilize SHISA5.
    • Sequence similarities

      Contains 5 EF-hand domains.
    • Cellular localization

      Nucleus membrane. Endoplasmic reticulum membrane. Nucleus. Endosome. Interaction with RBM22 induces relocalization from the cytoplasm to the nucleus. Translocated from the cytoplasm to the nucleus after heat shock cell treatment. Accumulates in cytoplasmic vesicle-like organelles after heat shock treatment, which may represent stress granules.
    • Information by UniProt
    • Database links

    • Alternative names

      • AIP1 antibody
      • ALG 2 antibody
      • ALG-2-interacting protein 1 antibody
      • ALG2 antibody
      • ALIX antibody
      • Apoptosis linked gene 2 antibody
      • Apoptosis linked gene 2 protein antibody
      • Apoptosis-linked gene 2 protein antibody
      • FLJ42309 antibody
      • FLJ46208 antibody
      • Hp95 antibody
      • KIAA1375 antibody
      • MA 3 antibody
      • MA3 antibody
      • MGC111017 antibody
      • MGC119050 antibody
      • MGC9123 antibody
      • PDCD 6 antibody
      • Pdcd6 antibody
      • PDCD6_HUMAN antibody
      • PEF 1B antibody
      • PEF1B antibody
      • Probable calcium binding protein ALG 2 antibody
      • Probable calcium binding protein ALG2 antibody
      • Probable calcium-binding protein ALG-2 antibody
      • Programmed cell death 6 antibody
      • Programmed cell death 6-interacting protein antibody
      • Programmed cell death protein 6 antibody
      • PS 2 antibody
      • PS2 antibody
      see all

    Images

    • All lanes : Anti-PDCD6/ALG-2 antibody [EPR3260] (ab133326) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 22 kDa

    References

    This product has been referenced in:

    • Liu X Up-regulation of miR-20a by HPV16 E6 exerts growth-promoting effects by targeting PDCD6 in cervical carcinoma cells. Biomed Pharmacother 102:996-1002 (2018). WB . Read more (PubMed: 29710555) »
    • Yuan L  et al. MiR-124 inhibits invasion and induces apoptosis of ovarian cancer cells by targeting programmed cell death 6. Oncol Lett 14:7311-7317 (2017). Read more (PubMed: 29344168) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab133326.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up