RecombinantRabMAb

Recombinant Anti-PDCD7 antibody [EPR8231] (ab131258)

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Immunocytochemistry/ Immunofluorescence - Anti-PDCD7 antibody [EPR8231] (ab131258)
  • Western blot - Anti-PDCD7 antibody [EPR8231] (ab131258)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD7 antibody [EPR8231] (ab131258)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD7 antibody [EPR8231] (ab131258)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD7 antibody [EPR8231] (ab131258)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD7 antibody [EPR8231] (ab131258)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD7 antibody [EPR8231] (ab131258)
  • OI-RD Scanning - Anti-PDCD7 antibody [EPR8231] (ab131258)
  • Anti-PDCD7 antibody [EPR8231] (ab131258)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8231] to PDCD7
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-PDCD7 antibody [EPR8231]
    See all PDCD7 primary antibodies

  • Description

    Rabbit monoclonal [EPR8231] to PDCD7

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide within Human PDCD7 aa 1-100. The exact sequence is proprietary.

  • Positive control

    • HeLa, Jurkat, MCF7 and Caco 2 cell lysates; MCF7 cells; Human kidney tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab131258 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/50 - 1/100.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/50 - 1/100.
Application notes
Is unsuitable for IP.

Target

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-PDCD7 antibody [EPR8231] (ab131258)
    Immunocytochemistry/ Immunofluorescence - Anti-PDCD7 antibody [EPR8231] (ab131258)
    Immunofluorescent analysis of MCF7 cells labelling PDCD7 with ab131258 at /150 dilution.
  • Western blot - Anti-PDCD7 antibody [EPR8231] (ab131258)
    Western blot - Anti-PDCD7 antibody [EPR8231] (ab131258)
    All lanes : Anti-PDCD7 antibody [EPR8231] (ab131258) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : MCF7 cell lysate
    Lane 4 : Caco 2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled Goat anti Rabbit IgG at 1/2000 dilution

    Predicted band size: 55 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD7 antibody [EPR8231] (ab131258)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD7 antibody [EPR8231] (ab131258)

    Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling PDCD7 with ab131258 antibody at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD7 antibody [EPR8231] (ab131258)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD7 antibody [EPR8231] (ab131258)

    Immunohistochemical analysis using ab131258 showing positive staining in Normal uterus tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD7 antibody [EPR8231] (ab131258)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD7 antibody [EPR8231] (ab131258)

    Immunohistochemical analysis using ab131258 showing positive staining in Normal brain tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD7 antibody [EPR8231] (ab131258)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD7 antibody [EPR8231] (ab131258)

    Immunohistochemical analysis using ab131258 showing positive staining in Normal breast tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD7 antibody [EPR8231] (ab131258)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD7 antibody [EPR8231] (ab131258)

    Immunohistochemical analysis using ab131258 showing positive staining in Normal colon tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-PDCD7 antibody [EPR8231] (ab131258)
    OI-RD Scanning - Anti-PDCD7 antibody [EPR8231] (ab131258)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-PDCD7 antibody [EPR8231] (ab131258)
    Anti-PDCD7 antibody [EPR8231] (ab131258)

References (1)

Publishing research using ab131258? Please let us know so that we can cite the reference in this datasheet.

ab131258 has been referenced in 1 publication.

  • Ishihara T  et al. Decreased number of Gemini of coiled bodies and U12 snRNA level in amyotrophic lateral sclerosis. Hum Mol Genet 22:4136-47 (2013). PubMed: 23740936

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