Overview

  • Product name

    Anti-PDCD7 antibody [EPR8231]
    See all PDCD7 primary antibodies
  • Description

    Rabbit monoclonal [EPR8231] to PDCD7
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human PDCD7 aa 1-100. The exact sequence is proprietary.

  • Positive control

    • HeLa, Jurkat, MCF7 and Caco 2 cell lysates; MCF7 cells; Human kidney tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab131258 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for IP.
  • Target

    Images

    • Immunofluorescent analysis of MCF7 cells labelling PDCD7 with ab131258 at /150 dilution.
    • All lanes : Anti-PDCD7 antibody [EPR8231] (ab131258) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : MCF7 cell lysate
      Lane 4 : Caco 2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled Goat anti Rabbit IgG at 1/2000 dilution

      Predicted band size: 55 kDa

    • Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling PDCD7 with ab131258 antibody at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemical analysis using ab131258 showing positive staining in Normal uterus tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemical analysis using ab131258 showing positive staining in Normal brain tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemical analysis using ab131258 showing positive staining in Normal breast tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemical analysis using ab131258 showing positive staining in Normal colon tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Ishihara T  et al. Decreased number of Gemini of coiled bodies and U12 snRNA level in amyotrophic lateral sclerosis. Hum Mol Genet 22:4136-47 (2013). Read more (PubMed: 23740936) »
    See 1 Publication for this product

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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