Recombinant Anti-PDCD7 antibody [EPR8231] (ab131258)

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Immunocytochemistry/ Immunofluorescence - Anti-PDCD7 antibody [EPR8231] (ab131258)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR8231] to PDCD7
Suitable for: WB, IHC-P, ICC/IF
Reacts with: Human

Carrier Free

Product name

Anti-PDCD7 antibody [EPR8231]
See all PDCD7 primary antibodies
Description

Rabbit monoclonal [EPR8231] to PDCD7
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: IP
Species reactivity

Reacts with: Human
Predicted to work with: Mouse, Rat
Immunogen

Synthetic peptide within Human PDCD7 aa 1-100. The exact sequence is proprietary.
Positive control
- HeLa, Jurkat, MCF7 and Caco 2 cell lysates; MCF7 cells; Human kidney tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (K_D)

K_D = 8.10 x 10 ^-11 M
Learn more about K_D
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR8231
Isotype

IgG
Research areas

Alternative Versions
- Anti-PDCD7 antibody [EPR8231] - BSA and Azide free (ab248393)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- MCF7 nuclear extract lysate (ab14860)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab131258 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
IHC-P		1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF		1/50 - 1/100.

Notes
WB 1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/50 - 1/100.

Application notes

Is unsuitable for IP.

Function

Promotes apoptosis when overexpressed.
Cellular localization

Nucleus.
Target information above from: UniProt accession Q8N8D1 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 10081 Human
- Entrez Gene: 50996 Mouse
- Entrez Gene: 363082 Rat
- Omim: 608138 Human
- SwissProt: Q8N8D1 Human
- SwissProt: Q9WTY1 Mouse
- Unigene: 458596 Human
- Unigene: 29193 Mouse
Alternative names
- Apoptosis-related protein ES18 antibody
- ES18 antibody
- HES18 antibody
- MGC22015 antibody
- Pdcd7 antibody
- PDCD7_HUMAN antibody
- Programmed cell death 7 antibody
- Programmed cell death protein 7 antibody
see all

Immunocytochemistry/ Immunofluorescence - Anti-PDCD7 antibody [EPR8231] (ab131258)

Immunofluorescent analysis of MCF7 cells labelling PDCD7 with ab131258 at /150 dilution.
Western blot - Anti-PDCD7 antibody [EPR8231] (ab131258)

All lanes : Anti-PDCD7 antibody [EPR8231] (ab131258) at 1/1000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : Caco 2 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled Goat anti Rabbit IgG at 1/2000 dilution

Predicted band size: 55 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD7 antibody [EPR8231] (ab131258)

Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling PDCD7 with ab131258 antibody at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD7 antibody [EPR8231] (ab131258)

Immunohistochemical analysis using ab131258 showing positive staining in Normal uterus tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD7 antibody [EPR8231] (ab131258)

Immunohistochemical analysis using ab131258 showing positive staining in Normal brain tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD7 antibody [EPR8231] (ab131258)

Immunohistochemical analysis using ab131258 showing positive staining in Normal breast tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD7 antibody [EPR8231] (ab131258)

Immunohistochemical analysis using ab131258 showing positive staining in Normal colon tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
OI-RD Scanning - Anti-PDCD7 antibody [EPR8231] (ab131258)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D
Anti-PDCD7 antibody [EPR8231] (ab131258)

Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

Datasheet download

Download

Publishing research using ab131258? Please let us know so that we can cite the reference in this datasheet.

ab131258 has been referenced in 1 publication.

Ishihara T et al. Decreased number of Gemini of coiled bodies and U12 snRNA level in amyotrophic lateral sclerosis. Hum Mol Genet 22:4136-47 (2013). PubMed: 23740936

Customer reviews and Q&As

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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Dissociation constant (KD)

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Isotype control

Positive Controls

Applications

The Abpromise guarantee

Target

Function

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

Datasheet download

References (1)

Customer reviews and Q&As

Dissociation constant (K_D)