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  1. Link

    pdcd7-antibody-epr8231-ab131258.pdf

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Cell Biology Apoptosis Extracellular Signals Death Ligands
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RecombinantRabMAb

Recombinant Anti-PDCD7 antibody [EPR8231] (ab131258)

  • Datasheet
  • SDS
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Immunocytochemistry/ Immunofluorescence - Anti-PDCD7 antibody [EPR8231] (ab131258)
  • Western blot - Anti-PDCD7 antibody [EPR8231] (ab131258)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD7 antibody [EPR8231] (ab131258)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD7 antibody [EPR8231] (ab131258)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD7 antibody [EPR8231] (ab131258)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD7 antibody [EPR8231] (ab131258)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD7 antibody [EPR8231] (ab131258)
  • OI-RD Scanning - Anti-PDCD7 antibody [EPR8231] (ab131258)
  • Anti-PDCD7 antibody [EPR8231] (ab131258)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8231] to PDCD7
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

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Overview

  • Product name

    Anti-PDCD7 antibody [EPR8231]
    See all PDCD7 primary antibodies
  • Description

    Rabbit monoclonal [EPR8231] to PDCD7
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human PDCD7 aa 1-100. The exact sequence is proprietary.

  • Positive control

    • HeLa, Jurkat, MCF7 and Caco 2 cell lysates; MCF7 cells; Human kidney tissue.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 8.10 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR8231
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Extracellular Signals
    • Death Ligands
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Splicing
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Apoptosis
    • Nuclear
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

Associated products

  • Alternative Versions

    • Anti-PDCD7 antibody [EPR8231] - BSA and Azide free (ab248393)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • MCF7 nuclear extract lysate (ab14860)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab131258 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/50 - 1/100.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/50 - 1/100.
Application notes
Is unsuitable for IP.

Target

  • Function

    Promotes apoptosis when overexpressed.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession Q8N8D1 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 10081 Human
    • Entrez Gene: 50996 Mouse
    • Entrez Gene: 363082 Rat
    • Omim: 608138 Human
    • SwissProt: Q8N8D1 Human
    • SwissProt: Q9WTY1 Mouse
    • Unigene: 458596 Human
    • Unigene: 29193 Mouse
    • Alternative names

      • Apoptosis-related protein ES18 antibody
      • ES18 antibody
      • HES18 antibody
      • MGC22015 antibody
      • Pdcd7 antibody
      • PDCD7_HUMAN antibody
      • Programmed cell death 7 antibody
      • Programmed cell death protein 7 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-PDCD7 antibody [EPR8231] (ab131258)
      Immunocytochemistry/ Immunofluorescence - Anti-PDCD7 antibody [EPR8231] (ab131258)
      Immunofluorescent analysis of MCF7 cells labelling PDCD7 with ab131258 at /150 dilution.
    • Western blot - Anti-PDCD7 antibody [EPR8231] (ab131258)
      Western blot - Anti-PDCD7 antibody [EPR8231] (ab131258)
      All lanes : Anti-PDCD7 antibody [EPR8231] (ab131258) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : MCF7 cell lysate
      Lane 4 : Caco 2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled Goat anti Rabbit IgG at 1/2000 dilution

      Predicted band size: 55 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD7 antibody [EPR8231] (ab131258)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD7 antibody [EPR8231] (ab131258)

      Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling PDCD7 with ab131258 antibody at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD7 antibody [EPR8231] (ab131258)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD7 antibody [EPR8231] (ab131258)

      Immunohistochemical analysis using ab131258 showing positive staining in Normal uterus tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD7 antibody [EPR8231] (ab131258)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD7 antibody [EPR8231] (ab131258)

      Immunohistochemical analysis using ab131258 showing positive staining in Normal brain tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD7 antibody [EPR8231] (ab131258)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD7 antibody [EPR8231] (ab131258)

      Immunohistochemical analysis using ab131258 showing positive staining in Normal breast tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD7 antibody [EPR8231] (ab131258)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD7 antibody [EPR8231] (ab131258)

      Immunohistochemical analysis using ab131258 showing positive staining in Normal colon tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • OI-RD Scanning - Anti-PDCD7 antibody [EPR8231] (ab131258)
      OI-RD Scanning - Anti-PDCD7 antibody [EPR8231] (ab131258)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-PDCD7 antibody [EPR8231] (ab131258)
      Anti-PDCD7 antibody [EPR8231] (ab131258)

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab131258? Please let us know so that we can cite the reference in this datasheet.

    ab131258 has been referenced in 1 publication.

    • Ishihara T  et al. Decreased number of Gemini of coiled bodies and U12 snRNA level in amyotrophic lateral sclerosis. Hum Mol Genet 22:4136-47 (2013). PubMed: 23740936

    Customer reviews and Q&As

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