Key features and details
- Rabbit polyclonal to PDCL
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-PDCL antibody
See all PDCL primary antibodies
DescriptionRabbit polyclonal to PDCL
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow
Synthetic peptide within Human PDCL aa 1-50. The exact sequence is proprietary.
Database link: Q13371
- WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 cell lysate. IP: HeLa cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab241283 was affinity purified using an epitope specific to PDCL immobilized on solid support.
Our Abpromise guarantee covers the use of ab241283 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/2000 - 1/10000.|
Sequence similaritiesBelongs to the phosducin family.
- Information by UniProt
- OTTHUMP00000022069 antibody
- PDCL antibody
- PHLOP1 antibody
All lanes : Anti-PDCL antibody (ab241283) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Exposure time: 10 seconds
Prepared using NETN lysis buffer.
PDCL was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241283 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab241283 at 1 µg/ml.
Lane 1: ab241283 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab241283 has not yet been referenced specifically in any publications.