Overview

  • Product name

    Anti-Pdd1 antibody - ChIP Grade
  • Description

    Rabbit polyclonal to Pdd1 - ChIP Grade
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IP, ChIPmore details
  • Species reactivity

    Reacts with: Tetrahymena
  • Immunogen

    Synthetic peptide corresponding to Tetrahymena Pdd1 aa 300-400 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab24392)

Properties

Applications

Our Abpromise guarantee covers the use of ab5338 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution.
WB 1/1000. Detects a band of approximately 64 kDa (predicted molecular weight: 57 kDa).
IP Use at an assay dependent dilution.
ChIP Use at an assay dependent concentration. PubMed: 20357003

Target

  • Relevance

    The PDD1 gene sequence predicts a novel polypeptide containing two copies of a chromodomain, a conserved domain found in a number of heterochromatin-associated proteins (Koonin et al. 1995). Consistent with this finding, Pdd1p localizes to electron-dense heterochromatic structures that concentrate at the periphery of macronuclear anlagen during the time when DNA deletion occurs.
  • Cellular localization

    Nuclear
  • Database links

    • Alternative names

      • p65 antibody
      • Pdd 1 antibody
      • Pdd 1p antibody
      • Pdd1p antibody
      • Programmed DNA degradation (pdd) antibody
      see all

    Images

    • ab5338 staining Pdd1 in wild-type Tetrahymena sp. cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in methanol. The primary antibody was diluted 1/250 in TBS + 1% Goat Serum and incubated with the sample for 18 hours at 4°C. The secondary antibody was Alexa Fluor® 488-conjugated Goat anti-Rabbit polyclonal, diluted 1/1000.

      See Abreview

    • All lanes : Anti-Pdd1 antibody - ChIP Grade (ab5338) at 1/1000 dilution

      Lane 1 : Wild-type Tetrahymena sp. Starved Tetrahymena, do not express pdd1p (hour 0)
      Lane 2 : Wild-type Tetrahymena sp. Conjugating Tetrahymena, peak of pdd1p expression (hour 9)

      Lysates/proteins at 15000 cells per lane.

      Secondary
      All lanes : HRP-conjugated Goat anti-Rabbit at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 57 kDa
      Observed band size: 64 kDa
      why is the actual band size different from the predicted?


      Exposure time: 30 seconds

      See Abreview

    References

    This product has been referenced in:

    • Suhren JH  et al. Negative Regulators of an RNAi-Heterochromatin Positive Feedback Loop Safeguard Somatic Genome Integrity in Tetrahymena. Cell Rep 18:2494-2507 (2017). Read more (PubMed: 28273462) »
    • Kataoka K & Mochizuki K Heterochromatin aggregation during DNA elimination in Tetrahymena is facilitated by a prion-like protein. J Cell Sci 130:480-489 (2017). ICC/IF ; Tetrahymena . Read more (PubMed: 27909245) »
    See all 15 Publications for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Application
    Western blot
    Sample
    Tetrahymena sp. Cell lysate - whole cell (wildtype)
    Loading amount
    15000 cells
    Specification
    wildtype
    Gel Running Conditions
    Reduced Denaturing (12%)
    Blocking step
    Milk as blocking agent for 20 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

    Abcam user community

    Verified customer

    Submitted Sep 22 2011

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Tetrahymena sp. Cell (wildtype)
    Specification
    wildtype
    Fixative
    Methanol
    Permeabilization
    No

    Abcam user community

    Verified customer

    Submitted Sep 22 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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