Product nameAnti-Pdd1 antibody - ChIP Grade
DescriptionRabbit polyclonal to Pdd1 - ChIP Grade
Tested applicationsSuitable for: ICC/IF, WB, IP, ChIPmore details
Species reactivityReacts with: Tetrahymena
Synthetic peptide derived from residues 300 - 400 of Tetrahymena sp. Pdd1.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
ChIP Related Products
Our Abpromise guarantee covers the use of ab5338 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent dilution.|
|WB||1/1000. Detects a band of approximately 64 kDa (predicted molecular weight: 57 kDa).|
|IP||Use at an assay dependent dilution.|
|ChIP||Use at an assay dependent concentration. PubMed: 20357003|
RelevanceThe PDD1 gene sequence predicts a novel polypeptide containing two copies of a chromodomain, a conserved domain found in a number of heterochromatin-associated proteins (Koonin et al. 1995). Consistent with this finding, Pdd1p localizes to electron-dense heterochromatic structures that concentrate at the periphery of macronuclear anlagen during the time when DNA deletion occurs.
- SwissProt: Q94996 Tetrahymena
- p65 antibody
- Pdd 1 antibody
- Pdd 1p antibody
ab5338 staining Pdd1 in wild-type Tetrahymena sp. cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in methanol. The primary antibody was diluted 1/250 in TBS + 1% Goat Serum and incubated with the sample for 18 hours at 4°C. The secondary antibody was Alexa Fluor® 488-conjugated Goat anti-Rabbit polyclonal, diluted 1/1000.
All lanes : Anti-Pdd1 antibody - ChIP Grade (ab5338) at 1/1000 dilution
Lane 1 : Wild-type Tetrahymena sp. Starved Tetrahymena, do not express pdd1p (hour 0)
Lane 2 : Wild-type Tetrahymena sp. Conjugating Tetrahymena, peak of pdd1p expression (hour 9)
Lysates/proteins at 15000 cells per lane.
All lanes : HRP-conjugated Goat anti-Rabbit at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 57 kDa
Observed band size: 64 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
This product has been referenced in:
- Suhren JH et al. Negative Regulators of an RNAi-Heterochromatin Positive Feedback Loop Safeguard Somatic Genome Integrity in Tetrahymena. Cell Rep 18:2494-2507 (2017). Read more (PubMed: 28273462) »
- Kataoka K & Mochizuki K Heterochromatin aggregation during DNA elimination in Tetrahymena is facilitated by a prion-like protein. J Cell Sci 130:480-489 (2017). ICC/IF ; Tetrahymena . Read more (PubMed: 27909245) »