Product nameAnti-PDDC1 antibody
See all PDDC1 primary antibodies
DescriptionRabbit polyclonal to PDDC1
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Human
antigen, corresponding to amino acids 119-220 of Human PDDC1.
- Human cerebral cortex tissue; RT 4 and U251 MG cell lysates
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab126492 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
|WB||1/250 - 1/500. Predicted molecular weight: 23 kDa.|
|IHC-P||1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Sequence similaritiesBelongs to the peptidase C56 family.
- Information by UniProt
- Parkinson disease 7 domain containing 1 antibody
- Parkinson disease 7 domain-containing protein 1 antibody
- Pddc1 antibody
- PDDC1_HUMAN antibody
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nuclei but not nucleoli. Recommended concentration of ab126492 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab126492 at 1/10 dilution staining PDDC1 in paraffin-embedded Human cerebral cortex tissue by Immunohistochemistry.
All lanes : Anti-PDDC1 antibody (ab126492) at 1/250 dilution
Lane 1 : RT 4 cell lysate
Lane 2 : U251 MG cell lysate
Lane 3 : Human plasma lysate
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate
Developed using the ECL technique.
Predicted band size: 23 kDa
ab126492 has not yet been referenced specifically in any publications.