Anti-PDDC1 antibody (ab126492)
Key features and details
- Rabbit polyclonal to PDDC1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PDDC1 antibody
See all PDDC1 primary antibodies -
Description
Rabbit polyclonal to PDDC1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human
-
Immunogen
antigen, corresponding to amino acids 119-220 of Human PDDC1.
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Positive control
- Human cerebral cortex tissue; RT 4 and U251 MG cell lysates
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab126492 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Human
|
| IHC-P |
Human
|
| WB |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
|
|
| WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 23 kDa.
|
|
| IHC-P |
1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 23 kDa. |
|
IHC-P
1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
-
Sequence similarities
Belongs to the peptidase C56 family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 347862 Human
- SwissProt: Q8NB37 Human
- Unigene: 218362 Human
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Alternative names
- Parkinson disease 7 domain containing 1 antibody
- Parkinson disease 7 domain-containing protein 1 antibody
- Pddc1 antibody
- PDDC1_HUMAN antibody
Images
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Immunocytochemistry/ Immunofluorescence - Anti-PDDC1 antibody (ab126492)Immunofluorescent staining of Human cell line U-2 OS shows positivity in nuclei but not nucleoli. Recommended concentration of ab126492 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDDC1 antibody (ab126492)ab126492 at 1/10 dilution staining PDDC1 in paraffin-embedded Human cerebral cortex tissue by Immunohistochemistry. -
Western blot - Anti-PDDC1 antibody (ab126492)All lanes : Anti-PDDC1 antibody (ab126492) at 1/250 dilution
Lane 1 : RT 4 cell lysate
Lane 2 : U251 MG cell lysate
Lane 3 : Human plasma lysate
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate
Developed using the ECL technique.
Predicted band size: 23 kDa
Protocols
Datasheets and documents
References (0)
ab126492 has not yet been referenced specifically in any publications.
