Anti-PDDC1 antibody (ab126492)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-PDDC1 antibody
See all PDDC1 primary antibodies -
Description
Rabbit polyclonal to PDDC1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen, corresponding to amino acids 119-220 of Human PDDC1.
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Positive control
- Human cerebral cortex tissue; RT 4 and U251 MG cell lysates
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab126492 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 1 - 4 µg/ml. Recommend PFA Fixation and Triton X-100 treatment |
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| WB | 1/250 - 1/500. Predicted molecular weight: 23 kDa. | |
| IHC-P | 1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Sequence similarities
Belongs to the peptidase C56 family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 347862 Human
- SwissProt: Q8NB37 Human
- Unigene: 218362 Human
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Alternative names
- Parkinson disease 7 domain containing 1 antibody
- Parkinson disease 7 domain-containing protein 1 antibody
- Pddc1 antibody
- PDDC1_HUMAN antibody
Images
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Immunocytochemistry/ Immunofluorescence - Anti-PDDC1 antibody (ab126492)Immunofluorescent staining of Human cell line U-2 OS shows positivity in nuclei but not nucleoli. Recommended concentration of ab126492 1-4 µg/ml. Cells treated with PFA/Triton X-100. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDDC1 antibody (ab126492)ab126492 at 1/10 dilution staining PDDC1 in paraffin-embedded Human cerebral cortex tissue by Immunohistochemistry. -
Western blot - Anti-PDDC1 antibody (ab126492)All lanes : Anti-PDDC1 antibody (ab126492) at 1/250 dilution
Lane 1 : RT 4 cell lysate
Lane 2 : U251 MG cell lysate
Lane 3 : Human plasma lysate
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate
Developed using the ECL technique.
Predicted band size: 23 kDa
Protocols
Datasheets and documents
References
ab126492 has not yet been referenced specifically in any publications.
