Overview

  • Product name

  • Description

    Rabbit polyclonal to PDDC1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    antigen, corresponding to amino acids 119-220 of Human PDDC1.

  • Positive control

    • Human cerebral cortex tissue; RT 4 and U251 MG cell lysates

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab126492 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

WB 1/250 - 1/500. Predicted molecular weight: 23 kDa.
IHC-P 1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in nuclei but not nucleoli. Recommended concentration of ab126492 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab126492 at 1/10 dilution staining PDDC1 in paraffin-embedded Human cerebral cortex tissue by Immunohistochemistry.
  • All lanes : Anti-PDDC1 antibody (ab126492) at 1/250 dilution

    Lane 1 : RT 4 cell lysate
    Lane 2 : U251 MG cell lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human liver tissue lysate
    Lane 5 : Human tonsil tissue lysate

    Developed using the ECL technique.

    Predicted band size: 23 kDa

References

ab126492 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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