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Signal Transduction Second Messenger Nucleotide Messenger cAMP
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Anti-PDE10A antibody - C-terminal (ab151454)

  • Datasheet
  • SDS
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Immunocytochemistry/ Immunofluorescence - Anti-PDE10A antibody - C-terminal (ab151454)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE10A antibody - C-terminal (ab151454)
  • Western blot - Anti-PDE10A antibody - C-terminal (ab151454)

Key features and details

  • Rabbit polyclonal to PDE10A - C-terminal
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant human PDE10A protein (ab101116)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-PDE10A antibody - C-terminal
    See all PDE10A primary antibodies
  • Description

    Rabbit polyclonal to PDE10A - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment corresponding to a region within C terminal end amino acids 547 and 779 of PDE10A (Q9Y233).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Hela cells and whole cell lysate, Cal27 xenograft tissue.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Second Messenger
    • Nucleotide Messenger
    • cAMP

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
  • Recombinant Protein

    • Recombinant human PDE10A protein (ab101116)

Applications

Our Abpromise guarantee covers the use of ab151454 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 88 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. Can hydrolyze both cAMP and cGMP, but has higher affinity for cAMP and is more efficient with cAMP as substrate.
  • Tissue specificity

    Abundant in the putamen and caudate nucleus regions of brain and testis, moderately expressed in the thyroid gland, pituitary gland, thalamus and cerebellum.
  • Pathway

    Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
    Purine metabolism; 3',5'-cyclic GMP degradation; GMP from 3',5'-cyclic GMP: step 1/1.
  • Sequence similarities

    Belongs to the cyclic nucleotide phosphodiesterase family.
    Contains 2 GAF domains.
  • Domain

    The tandem GAF domains bind cAMP, and regulate enzyme activity. The binding of cAMP stimulates enzyme activity.
    Composed of a C-terminal catalytic domain containing two divalent metal sites and an N-terminal regulatory domain which contains one cyclic nucleotide-binding region.
  • Cellular localization

    Cytoplasm. Located mostly to soluble cellular fractions.
  • Target information above from: UniProt accession Q9Y233 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 10846 Human
    • Entrez Gene: 23984 Mouse
    • Entrez Gene: 63885 Rat
    • Omim: 610652 Human
    • SwissProt: Q9Y233 Human
    • SwissProt: Q8CA95 Mouse
    • SwissProt: Q9QYJ6 Rat
    • Unigene: 348762 Human
    • Unigene: 87161 Mouse
    • Unigene: 44869 Rat
    see all
  • Alternative names

    • 5''-cyclic phosphodiesterase 10A antibody
    • cAMP and cAMP inhibited cGMP 3'5' cyclic phosphodiesterase 10A antibody
    • cAMP and cAMP-inhibited cGMP 3'' antibody
    • FLJ11894 antibody
    • HSPDE 10A antibody
    • HSPDE10A antibody
    • PDE 10A antibody
    • PDE10_HUMAN antibody
    • Pde10a antibody
    • Phosphodiesterase 10A antibody
    • Phosphodiesterase 10A1 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-PDE10A antibody - C-terminal (ab151454)
    Immunocytochemistry/ Immunofluorescence - Anti-PDE10A antibody - C-terminal (ab151454)
    Immunofluorescence analysis of methanol fixed HeLa cells labeling PDE10A with ab151454 at 1/200. The lower panel shows costaining with Hoechst 33342.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE10A antibody - C-terminal (ab151454)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE10A antibody - C-terminal (ab151454)
    Immunohistochemical analysis of paraffin embedded Human Cal27 xenograft tissue labeling PDE10A with ab151454 at 1/500.
  • Western blot - Anti-PDE10A antibody - C-terminal (ab151454)
    Western blot - Anti-PDE10A antibody - C-terminal (ab151454)
    Anti-PDE10A antibody - C-terminal (ab151454) at 1/3000 dilution + Hela whole cell lysate at 30 µg

    Predicted band size: 88 kDa



    7.5% SDS PAGE

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab151454? Please let us know so that we can cite the reference in this datasheet.

    ab151454 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
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    Immunocytochemistry/ Immunofluorescence abreview for Anti-PDE10A antibody - C-terminal

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Kidney)
    Permeabilization
    Yes - Citrat
    Specification
    Kidney
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 14 2018

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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