Key features and details
- Rabbit polyclonal to PDE10A - C-terminal
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PDE10A antibody - C-terminal
See all PDE10A primary antibodies
DescriptionRabbit polyclonal to PDE10A - C-terminal
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
- Hela cells and whole cell lysate, Cal27 xenograft tissue.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab151454 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 88 kDa.|
|IHC-P||1/100 - 1/1000.|
|ICC/IF||1/100 - 1/1000.|
FunctionPlays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. Can hydrolyze both cAMP and cGMP, but has higher affinity for cAMP and is more efficient with cAMP as substrate.
Tissue specificityAbundant in the putamen and caudate nucleus regions of brain and testis, moderately expressed in the thyroid gland, pituitary gland, thalamus and cerebellum.
PathwayPurine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
Purine metabolism; 3',5'-cyclic GMP degradation; GMP from 3',5'-cyclic GMP: step 1/1.
Sequence similaritiesBelongs to the cyclic nucleotide phosphodiesterase family.
Contains 2 GAF domains.
DomainThe tandem GAF domains bind cAMP, and regulate enzyme activity. The binding of cAMP stimulates enzyme activity.
Composed of a C-terminal catalytic domain containing two divalent metal sites and an N-terminal regulatory domain which contains one cyclic nucleotide-binding region.
Cellular localizationCytoplasm. Located mostly to soluble cellular fractions.
- Information by UniProt
- 5''-cyclic phosphodiesterase 10A antibody
- cAMP and cAMP inhibited cGMP 3'5' cyclic phosphodiesterase 10A antibody
- cAMP and cAMP-inhibited cGMP 3'' antibody
Immunofluorescence analysis of methanol fixed HeLa cells labeling PDE10A with ab151454 at 1/200. The lower panel shows costaining with Hoechst 33342.
Immunohistochemical analysis of paraffin embedded Human Cal27 xenograft tissue labeling PDE10A with ab151454 at 1/500.
Anti-PDE10A antibody - C-terminal (ab151454) at 1/3000 dilution + Hela whole cell lysate at 30 µg
Predicted band size: 88 kDa
7.5% SDS PAGE
ab151454 has not yet been referenced specifically in any publications.