Anti-PDE10A antibody - C-terminal (ab151454)

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Overview

  • Product name

    Anti-PDE10A antibody - C-terminal
    See all PDE10A primary antibodies

  • Description

    Rabbit polyclonal to PDE10A - C-terminal

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Recombinant fragment corresponding to a region within C terminal end amino acids 547 and 779 of PDE10A (Q9Y233).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Hela cells and whole cell lysate, Cal27 xenograft tissue.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin
    Constituents: PBS, 20% Glycerol
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab151454 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 88 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. Can hydrolyze both cAMP and cGMP, but has higher affinity for cAMP and is more efficient with cAMP as substrate.

  • Tissue specificity

    Abundant in the putamen and caudate nucleus regions of brain and testis, moderately expressed in the thyroid gland, pituitary gland, thalamus and cerebellum.

  • Pathway

    Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
    Purine metabolism; 3',5'-cyclic GMP degradation; GMP from 3',5'-cyclic GMP: step 1/1.

  • Sequence similarities

    Belongs to the cyclic nucleotide phosphodiesterase family.
    Contains 2 GAF domains.

  • Domain

    The tandem GAF domains bind cAMP, and regulate enzyme activity. The binding of cAMP stimulates enzyme activity.
    Composed of a C-terminal catalytic domain containing two divalent metal sites and an N-terminal regulatory domain which contains one cyclic nucleotide-binding region.

  • Cellular localization

    Cytoplasm. Located mostly to soluble cellular fractions.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 5''-cyclic phosphodiesterase 10A antibody
    • cAMP and cAMP inhibited cGMP 3'5' cyclic phosphodiesterase 10A antibody
    • cAMP and cAMP-inhibited cGMP 3'' antibody
    • FLJ11894 antibody
    • HSPDE 10A antibody
    • HSPDE10A antibody
    • PDE 10A antibody
    • PDE10_HUMAN antibody
    • Pde10a antibody
    • Phosphodiesterase 10A antibody
    • Phosphodiesterase 10A1 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-PDE10A antibody - C-terminal (ab151454)
    Immunocytochemistry/ Immunofluorescence - Anti-PDE10A antibody - C-terminal (ab151454)
    Immunofluorescence analysis of methanol fixed HeLa cells labeling PDE10A with ab151454 at 1/200. The lower panel shows costaining with Hoechst 33342.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE10A antibody - C-terminal (ab151454)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE10A antibody - C-terminal (ab151454)
    Immunohistochemical analysis of paraffin embedded Human Cal27 xenograft tissue labeling PDE10A with ab151454 at 1/500.
  • Western blot - Anti-PDE10A antibody - C-terminal (ab151454)
    Western blot - Anti-PDE10A antibody - C-terminal (ab151454)
    Anti-PDE10A antibody - C-terminal (ab151454) at 1/3000 dilution + Hela whole cell lysate at 30 µg

    Predicted band size: 88 kDa



    7.5% SDS PAGE

References

ab151454 has not yet been referenced specifically in any publications.

Publishing research using ab151454? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Immunocytochemistry/ Immunofluorescence abreview for Anti-PDE10A antibody - C-terminal

 Good
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Kidney)
Permeabilization
Yes - Citrat
Specification
Kidney
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde
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Submitted Jun 14 2018

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